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Protein

Plexin-B2

Gene

Plxnb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development. Regulates the migration of cerebellar granule cells in the developing brain. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. May modulate the activity of RAC1 and CDC42. Down-regulates macrophage migration in wound-healing assays (in vitro).4 Publications

GO - Molecular functioni

  • semaphorin receptor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Plexin-B2
Gene namesi
Name:Plxnb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2154239 Plxnb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 1201ExtracellularSequence analysisAdd BLAST1182
Transmembranei1202 – 1222HelicalSequence analysisAdd BLAST21
Topological domaini1223 – 1842CytoplasmicSequence analysisAdd BLAST620

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryonic and perinatal lethality, due to defects in brain and neural tube development. Mice exhibit abnormal cortical layering and defective migration and differentiation of several subtypes of cortical neurons. Cranial neural folds fail to converge in most embryos, leading to an open neural tube and exencephaly. Likewise, mice exhibit defects in the embryonic development of the cerebellum and the olfactory bulb.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000041571020 – 1842Plexin-B2Add BLAST1823

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi78 ↔ 87PROSITE-ProRule annotation
Disulfide bondi112 ↔ 120PROSITE-ProRule annotation
Glycosylationi127N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi242N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi250 ↔ 366PROSITE-ProRule annotation
Disulfide bondi266 ↔ 313PROSITE-ProRule annotation
Disulfide bondi331 ↔ 353PROSITE-ProRule annotation
Glycosylationi393N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi451N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi471 ↔ 488PROSITE-ProRule annotation
Disulfide bondi477 ↔ 520PROSITE-ProRule annotation
Disulfide bondi480 ↔ 497PROSITE-ProRule annotation
Disulfide bondi491 ↔ 503PROSITE-ProRule annotation
Disulfide bondi557 ↔ 576PROSITE-ProRule annotation
Glycosylationi798N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi919N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1053N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1072N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei1240PhosphoserineCombined sources1
Modified residuei1248PhosphoserineCombined sources1
Modified residuei1574PhosphoserineCombined sources1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei1168 – 1169Cleavage; by proprotein convertasesBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiB2RXS4
PaxDbiB2RXS4
PeptideAtlasiB2RXS4
PRIDEiB2RXS4

PTM databases

iPTMnetiB2RXS4
PhosphoSitePlusiB2RXS4
SwissPalmiB2RXS4

Expressioni

Tissue specificityi

Detected in macrophages from spleen and bone marrow (at protein level). Detected in granule cells in the developing cerebellum, dentate gyrus and olfactory bulb.2 Publications

Gene expression databases

BgeeiENSMUSG00000036606 Expressed in 326 organ(s), highest expression level in pyloric antrum
GenevisibleiB2RXS4 MM

Interactioni

Subunit structurei

Monomer, and heterodimer with PLXNB1. Binds MET, ARHGEF11 and ARHGEF12. May also interact with MST1R (By similarity). Interacts with SEMA4C, SEMA4D and SEMA4G.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Arhgef11Q68FM72EBI-8496724,EBI-2365869

Protein-protein interaction databases

BioGridi228283, 2 interactors
IntActiB2RXS4, 1 interactor
MINTiB2RXS4
STRINGi10090.ENSMUSP00000051731

Structurei

Secondary structure

11842
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliB2RXS4
SMRiB2RXS4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 468SemaPROSITE-ProRule annotationAdd BLAST449
Domaini806 – 895IPT/TIG 1Add BLAST90
Domaini898 – 982IPT/TIG 2Add BLAST85
Domaini986 – 1095IPT/TIG 3Add BLAST110

Sequence similaritiesi

Belongs to the plexin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410KCS6 Eukaryota
ENOG411131Q LUCA
GeneTreeiENSGT00760000119048
HOGENOMiHOG000231376
HOVERGENiHBG053404
InParanoidiB2RXS4
KOiK06821
OMAiKPFHGDI
OrthoDBiEOG091G00EK
PhylomeDBiB2RXS4
TreeFamiTF312962

Family and domain databases

Gene3Di2.130.10.10, 1 hit
2.60.40.10, 3 hits
InterProiView protein in InterPro
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
IPR031148 Plexin
IPR013548 Plexin_cytoplasmic_RasGAP_dom
IPR002165 Plexin_repeat
IPR016201 PSI
IPR008936 Rho_GTPase_activation_prot
IPR001627 Semap_dom
IPR036352 Semap_dom_sf
IPR015943 WD40/YVTN_repeat-like_dom_sf
PANTHERiPTHR22625 PTHR22625, 1 hit
PfamiView protein in Pfam
PF08337 Plexin_cytopl, 1 hit
PF01437 PSI, 2 hits
PF01403 Sema, 1 hit
PF01833 TIG, 3 hits
SMARTiView protein in SMART
SM00429 IPT, 3 hits
SM00423 PSI, 3 hits
SM00630 Sema, 1 hit
SUPFAMiSSF101912 SSF101912, 1 hit
SSF48350 SSF48350, 2 hits
SSF81296 SSF81296, 3 hits
PROSITEiView protein in PROSITE
PS51004 SEMA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2RXS4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALPLWALTF LGLTGLGLSL RSRKPESFRS ETELNHLAVD EVTGVVYVGA
60 70 80 90 100
VNALYQLSAD LHVQQHVVTG PFMDNKKCTP PIEASQCHEA VLTDNFNQLL
110 120 130 140 150
LLDPPGKRLV ECGSLFKGIC ALRAMSNISV RLFYEDGSGE KSFVASNDER
160 170 180 190 200
VATVGLVTST RPDGERVLFV GKGNGPHDNG IIVSTRLLDR AEGREAFEAY
210 220 230 240 250
SDHTTFKAGY LSTNTQQFVA AFEDDFYVFF VFNHQDKHPA KNRTLLARMC
260 270 280 290 300
KDDPSYYSYV EMDLQCQDPS DPQDSAFGTC LAASVATSGA GRALYAVFSR
310 320 330 340 350
DGRSTGGPGA GLCVFPLDKV REKIEANRNA CYTGAREAGR TIFYKPFHGE
360 370 380 390 400
IQCGGHLIGA SESFPCGSEH LPYPLGSRDG LVATAVLHRG GLNLTAVTVT
410 420 430 440 450
AENDHTVAFL GTSDGRILKV YLAPDGTSAE YGSIPVDINK KIKQDLALSG
460 470 480 490 500
NLSSLYAMTQ DKVFRLPVQE CLSYVTCAQC RDSQDPYCGW CVIEGRCTRK
510 520 530 540 550
SECSRAEETG HWLWSREKSC VAITDAFPQN MSRRAQGEVR LSVSPLPTLT
560 570 580 590 600
EDDELLCLFG DSPPHPARVE DDTVICNSPS SIPSTPPGQD HVDVSIQLLL
610 620 630 640 650
KSGSVFLTSH QYPFYDCREA MSLVENLPCI SCASNRWTCQ WDLQYYECRE
660 670 680 690 700
ASPNPEEGII RAHMEDNCPQ FLAPDPLVIP MNHETEVTFQ GKNLETVKVS
710 720 730 740 750
SLYVGSELLN FEETVTMHES DTFSFRTPKL SHDGNETLPL HLYVKSFGKN
760 770 780 790 800
IDSKLQVTLY NCSFGRSDCS LCLAADPAYR CVWCRGQNRC VYEALCSNVT
810 820 830 840 850
SECPPPVITR IQPETGPLGG GILVTIHGSN LGVKADDVKK ITVAGQNCAF
860 870 880 890 900
EPRGYSVSTR IVCAIEASEM PFTGGIEVDV NGKLGHSPPH VQFTYQQPQP
910 920 930 940 950
LSVEPRQGPQ AGGTTLTING THLDTGSKED VRVTLNDVPC EVTKFGAQLQ
960 970 980 990 1000
CVTGQQLAPG QVTLEIYYGG SRVPSPGISF TYCENPMIRA FEPLRSFVSG
1010 1020 1030 1040 1050
GRSINVTGQG FSLIQKFAMV VIAEPLRSWR RRRREAGALE RVTVEGMEYV
1060 1070 1080 1090 1100
FYNDTKVVFL SPAVPEEPEA YNLTVLIRMD GHCAPLRTEA GVFEYVADPT
1110 1120 1130 1140 1150
FENFTGGVKK QVNKLIHARG TNLNKAMTLE EAEAFVGAER CIMKTLTETD
1160 1170 1180 1190 1200
LYCEPPEVQP PPKRRQKRDT AHNLPEFIVK FGSREWVLGR VEYDTRASDV
1210 1220 1230 1240 1250
PLSLILPLVM VPMVFIIVVS IYCYWRKSQQ AEREYEKIKS QLEGLEESVR
1260 1270 1280 1290 1300
DRCKKEFTDL MIEMEDQTND VHEAGIPTLD YKTYTDRVFF LPSKDGDKDV
1310 1320 1330 1340 1350
MITGKLDIPE SRRPIVEQAL YQFSNLLNSK SFLINFIHTL ENQREFSARA
1360 1370 1380 1390 1400
KVYFASLLTV ALHGKLEYYT DIMRTLFLEL MEQYVVAKNP KLMLRRSETV
1410 1420 1430 1440 1450
VERMLSNWMS ICLYQYLKDS AGEPLYKLFK AIKHQVEKGP VDAVQKKAKY
1460 1470 1480 1490 1500
TLNDTGLLGD DVEYAPLTVS VIVQDEGIDA IPVKVLNCDT ISQVKEKIID
1510 1520 1530 1540 1550
QVYRTQPCSC WPKPDSVVLE WRPGSTAQIL SDLDLTSQRE GRWKRINTLM
1560 1570 1580 1590 1600
HYNVRDGATL ILSKVGVSQQ PEDSQQDLPG ERHALLEEEN RVWHLVRPTD
1610 1620 1630 1640 1650
EVDEGKSKRG SMKEKERTKA ITEIYLTRLL SVKGTLQQFV DNFFQSVLAP
1660 1670 1680 1690 1700
GHAVPPAVKY FFDFLDEQAE KHDIRDEDTI HIWKTNSLPL RFWVNILKNP
1710 1720 1730 1740 1750
HFIFDVHVHE VVDASLSVIA QTFMDACTRT EHKLSRDSPS NKLLYAKEIS
1760 1770 1780 1790 1800
TYKKMVEDYY KGIRQMVQVS DQDMNTHLAE ISRAHTDSLN TLVALHQLYQ
1810 1820 1830 1840
YTQKYYDEII NALEEDPAAQ KMQLAFRLQQ IAAALENKVT DL
Length:1,842
Mass (Da):206,230
Last modified:July 1, 2008 - v1
Checksum:i0D445E7354C107FF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1382E → G in BAE27981 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147538 mRNA Translation: BAE27981.1
AC113069 Genomic DNA No translation available.
AC160538 Genomic DNA No translation available.
CH466550 Genomic DNA Translation: EDL04367.1
BC157960 mRNA Translation: AAI57961.1
BC157961 mRNA Translation: AAI57962.1
BC158086 mRNA Translation: AAI58087.1
BC172162 mRNA Translation: AAI72162.1
CCDSiCCDS49698.1
RefSeqiNP_001152993.1, NM_001159521.2
NP_001271435.1, NM_001284506.1
NP_620088.2, NM_138749.3
XP_006520475.1, XM_006520412.1
XP_006520476.1, XM_006520413.1
XP_011243751.1, XM_011245449.1
XP_011243752.1, XM_011245450.2
XP_011243753.1, XM_011245451.2
UniGeneiMm.267514

Genome annotation databases

EnsembliENSMUST00000060808; ENSMUSP00000051731; ENSMUSG00000036606
ENSMUST00000109331; ENSMUSP00000104955; ENSMUSG00000036606
GeneIDi140570
KEGGimmu:140570
UCSCiuc007xfr.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147538 mRNA Translation: BAE27981.1
AC113069 Genomic DNA No translation available.
AC160538 Genomic DNA No translation available.
CH466550 Genomic DNA Translation: EDL04367.1
BC157960 mRNA Translation: AAI57961.1
BC157961 mRNA Translation: AAI57962.1
BC158086 mRNA Translation: AAI58087.1
BC172162 mRNA Translation: AAI72162.1
CCDSiCCDS49698.1
RefSeqiNP_001152993.1, NM_001159521.2
NP_001271435.1, NM_001284506.1
NP_620088.2, NM_138749.3
XP_006520475.1, XM_006520412.1
XP_006520476.1, XM_006520413.1
XP_011243751.1, XM_011245449.1
XP_011243752.1, XM_011245450.2
XP_011243753.1, XM_011245451.2
UniGeneiMm.267514

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5E6PX-ray3.21A1226-1842[»]
ProteinModelPortaliB2RXS4
SMRiB2RXS4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228283, 2 interactors
IntActiB2RXS4, 1 interactor
MINTiB2RXS4
STRINGi10090.ENSMUSP00000051731

PTM databases

iPTMnetiB2RXS4
PhosphoSitePlusiB2RXS4
SwissPalmiB2RXS4

Proteomic databases

MaxQBiB2RXS4
PaxDbiB2RXS4
PeptideAtlasiB2RXS4
PRIDEiB2RXS4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060808; ENSMUSP00000051731; ENSMUSG00000036606
ENSMUST00000109331; ENSMUSP00000104955; ENSMUSG00000036606
GeneIDi140570
KEGGimmu:140570
UCSCiuc007xfr.2 mouse

Organism-specific databases

CTDi23654
MGIiMGI:2154239 Plxnb2

Phylogenomic databases

eggNOGiENOG410KCS6 Eukaryota
ENOG411131Q LUCA
GeneTreeiENSGT00760000119048
HOGENOMiHOG000231376
HOVERGENiHBG053404
InParanoidiB2RXS4
KOiK06821
OMAiKPFHGDI
OrthoDBiEOG091G00EK
PhylomeDBiB2RXS4
TreeFamiTF312962

Miscellaneous databases

ChiTaRSiPlxnb2 mouse
PROiPR:B2RXS4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036606 Expressed in 326 organ(s), highest expression level in pyloric antrum
GenevisibleiB2RXS4 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
2.60.40.10, 3 hits
InterProiView protein in InterPro
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
IPR031148 Plexin
IPR013548 Plexin_cytoplasmic_RasGAP_dom
IPR002165 Plexin_repeat
IPR016201 PSI
IPR008936 Rho_GTPase_activation_prot
IPR001627 Semap_dom
IPR036352 Semap_dom_sf
IPR015943 WD40/YVTN_repeat-like_dom_sf
PANTHERiPTHR22625 PTHR22625, 1 hit
PfamiView protein in Pfam
PF08337 Plexin_cytopl, 1 hit
PF01437 PSI, 2 hits
PF01403 Sema, 1 hit
PF01833 TIG, 3 hits
SMARTiView protein in SMART
SM00429 IPT, 3 hits
SM00423 PSI, 3 hits
SM00630 Sema, 1 hit
SUPFAMiSSF101912 SSF101912, 1 hit
SSF48350 SSF48350, 2 hits
SSF81296 SSF81296, 3 hits
PROSITEiView protein in PROSITE
PS51004 SEMA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPLXB2_MOUSE
AccessioniPrimary (citable) accession number: B2RXS4
Secondary accession number(s): Q3UH76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: July 1, 2008
Last modified: September 12, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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