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Protein

Receptor-type tyrosine-protein phosphatase beta

Gene

Ptprb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells and this requires the presence of plakoglobin.6 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1871SubstrateBy similarity1
Active sitei1905Phosphocysteine intermediatePROSITE-ProRule annotation1
Binding sitei1949SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: UniProtKB
  • dephosphorylation Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processAngiogenesis

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase beta (EC:3.1.3.48)
Short name:
Protein-tyrosine phosphatase beta
Short name:
R-PTP-beta
Alternative name(s):
Vascular endothelial protein tyrosine phosphatase
Short name:
VE-PTP
Gene namesi
Name:Ptprb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97809 Ptprb

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 1622ExtracellularSequence analysisAdd BLAST1600
Transmembranei1623 – 1643HelicalSequence analysisAdd BLAST21
Topological domaini1644 – 1997CytoplasmicSequence analysisAdd BLAST354

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show severe cardiovascular defects and embryonic lethality by E10. Vasculogenesis occurs normally however, angiogenesis is abnormal. Angiogenic defects are most pronounced in the yolk sac and include a complete failure to elaborate the primitive vascular scaffold into higher-order branched arteries, veins, and capillaries.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1911R → A: Loss of activity and dephosphorylation of CDH5. 1 Publication1
Mutagenesisi1982Y → F: Loss of tyrosine phosphorylation. Abolishes interaction with FYN and GRB2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000039040123 – 1998Receptor-type tyrosine-protein phosphatase betaAdd BLAST1976

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi53N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi173N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi199N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi268N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi415N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi422N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi480N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi575N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi599N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi653N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi830N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1041N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1097N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1164N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1186N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1213N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1275N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1368N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1471N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1475N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1519N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei1982Phosphotyrosine1 Publication1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiB2RU80
PaxDbiB2RU80
PeptideAtlasiB2RU80
PRIDEiB2RU80

PTM databases

iPTMnetiB2RU80
PhosphoSitePlusiB2RU80

Expressioni

Tissue specificityi

Expression is very high in the vasculature of lung, spleen, and kidney, as well as in the heart valves, and is also present in the endothelium of arterioles and venules. Also expressed in tumor vasculature.3 Publications

Developmental stagei

Expressed in both arterial and venous vascular endothelium in embryos, although more strongly in arterial vessels. Highly expressed in the developing outflow tract of the heart and later is expressed in developing heart valves.3 Publications

Gene expression databases

BgeeiENSMUSG00000020154 Expressed in 245 organ(s), highest expression level in lung
ExpressionAtlasiB2RU80 baseline and differential
GenevisibleiB2RU80 MM

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with TEK (PubMed:10557082, PubMed:19451274). Interacts via fibronectin type-III 17 domain with CDH5 (PubMed:19015309). Detected in a complex with CNTN1 and NRCAM (PubMed:11564762). Interacts (phosphorylated form) with FYN and GRB2 (PubMed:20398064).By similarity6 Publications

Protein-protein interaction databases

IntActiB2RU80, 2 interactors
MINTiB2RU80
STRINGi10090.ENSMUSP00000089805

Structurei

3D structure databases

ProteinModelPortaliB2RU80
SMRiB2RU80
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 109Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST87
Domaini113 – 206Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST94
Domaini207 – 291Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST85
Domaini292 – 384Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST93
Domaini378 – 466Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST89
Domaini470 – 556Fibronectin type-III 6PROSITE-ProRule annotationAdd BLAST87
Domaini557 – 642Fibronectin type-III 7PROSITE-ProRule annotationAdd BLAST86
Domaini643 – 733Fibronectin type-III 8PROSITE-ProRule annotationAdd BLAST91
Domaini734 – 821Fibronectin type-III 9PROSITE-ProRule annotationAdd BLAST88
Domaini822 – 913Fibronectin type-III 10PROSITE-ProRule annotationAdd BLAST92
Domaini908 – 994Fibronectin type-III 11PROSITE-ProRule annotationAdd BLAST87
Domaini995 – 1088Fibronectin type-III 12PROSITE-ProRule annotationAdd BLAST94
Domaini1086 – 1173Fibronectin type-III 13PROSITE-ProRule annotationAdd BLAST88
Domaini1176 – 1263Fibronectin type-III 14PROSITE-ProRule annotationAdd BLAST88
Domaini1264 – 1357Fibronectin type-III 15PROSITE-ProRule annotationAdd BLAST94
Domaini1358 – 1449Fibronectin type-III 16PROSITE-ProRule annotationAdd BLAST92
Domaini1449 – 1551Fibronectin type-III 17PROSITE-ProRule annotationAdd BLAST103
Domaini1704 – 1964Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST261

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1905 – 1911Substrate bindingBy similarity7

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0791 Eukaryota
COG5599 LUCA
GeneTreeiENSGT00930000150847
HOGENOMiHOG000060224
HOVERGENiHBG053759
InParanoidiB2RU80
KOiK05694
OMAiPHIRVNK
OrthoDBiEOG091G00JF
PhylomeDBiB2RU80
TreeFamiTF351926

Family and domain databases

CDDicd00063 FN3, 12 hits
Gene3Di2.60.40.10, 15 hits
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR029021 Prot-tyrosine_phosphatase-like
IPR000242 PTPase_domain
IPR016130 Tyr_Pase_AS
IPR003595 Tyr_Pase_cat
IPR000387 TYR_PHOSPHATASE_dom
PfamiView protein in Pfam
PF00041 fn3, 15 hits
PF00102 Y_phosphatase, 1 hit
PRINTSiPR00700 PRTYPHPHTASE
SMARTiView protein in SMART
SM00060 FN3, 16 hits
SM00194 PTPc, 1 hit
SM00404 PTPc_motif, 1 hit
SUPFAMiSSF49265 SSF49265, 16 hits
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 12 hits
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50055 TYR_PHOSPHATASE_PTP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

B2RU80-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRHGALTAL WITLSVVQTG VAEQVKCNFT LLESRVSSLS ASIQWRTFAS
60 70 80 90 100
PCNFSLIYSS DTSGPMWCHP IRIDNFTYGC NPKDLQAGTV YNFRIVSLDG
110 120 130 140 150
EESTLVLQTD PLPPARFEVN REKTASTTLQ VRWTPSSGKV SWYEVQLFDH
160 170 180 190 200
NNQKIQEVQV QESTTWSQYT FLNLTEGNSY KVAITAVSGE KRSFPVYING
210 220 230 240 250
STVPSPVKDL GISPNPNSLL ISWSRGSGNV EQYRLVLMDK GAIVQDTNVD
260 270 280 290 300
RRDTSYAFHE LTPGHLYNLT IVTMASGLQN SRWKLVRTAP MEVSNLKVTN
310 320 330 340 350
DGRLTSLNVK WQKPPGDVDS YSITLSHQGT IKESKTLAPP VTETQFKDLV
360 370 380 390 400
PGRLYQVTIS CISGELSAEK SAAGRTVPEK VRNLVSYNEI WMKSFTVNWT
410 420 430 440 450
PPAGDWEHYR IVLFNESLVL LNTTVGKEET HYALDGLELI PGRQYEIEVI
460 470 480 490 500
VESGNLRNSE RCQGRTVPLA VLQLRVKHAN ETSLGITWRA PLGEWEKYII
510 520 530 540 550
SLMDRELLVI HKSLSKDAKE FTFTDLMPGR NYKATVTSMS GDLKQSSSIK
560 570 580 590 600
GRTVPAQVTD LHVNNQGMTS SLFTNWTKAL GDVEFYQVLL IHENVVVKNE
610 620 630 640 650
SVSSDTSRYS FRALKPGSLY SVVVTTVSGG ISSRQVVAEG RTVPSSVSGV
660 670 680 690 700
TVNNSGRNDY LSVSWLPAPG EVDHYVVSLS HEGKVDQFLI IAKSVSECSF
710 720 730 740 750
SSLTPGRLYN VTVTTKSGNY ASHSFTEERT VPDKVQGISV SNSARSDYLK
760 770 780 790 800
VSWVHATGDF DHYEVTIKNR ESFIQTKTIP KSENECEFIE LVPGRLYSVT
810 820 830 840 850
VSTKSGQYEA SEQGTGRTIP EPVKDLTLLN RSTEDLHVTW SRANGDVDQY
860 870 880 890 900
EVQLLFNDMK VFPHIHLVNT ATEYKFTALT PGRHYKILVL TISGDVQQSA
910 920 930 940 950
FIEGLTVPST VKNIHISANG ATDRLMVTWS PGGGDVDSYV VSAFRQDEKV
960 970 980 990 1000
DSQTIPKHAS EHTFHRLEAG AKYRIAIVSV SGSLRNQIDA LGQTVPASVQ
1010 1020 1030 1040 1050
GVVAANAYSS NSLTVSWQKA LGVAERYDIL LLNENGLLLS NVSEPATARQ
1060 1070 1080 1090 1100
HKFEDLTPGK KYKMQILTVS GGLFSKESQA EGRTVPAAVT NLRITENSSR
1110 1120 1130 1140 1150
YLSFGWTASE GELSWYNIFL YNPDRTLQER AQVDPLVQSF SFQNLLQGRM
1160 1170 1180 1190 1200
YKMVIVTHSG ELSNESFIFG RTVPAAVNHL KGSHRNTTDS LWFSWSPASG
1210 1220 1230 1240 1250
DFDFYELILY NPNGTKKENW KEKDVTEWRF QGLVPGRKYT LYVVTHSGDL
1260 1270 1280 1290 1300
SNKVTGEGRT APSPPSLLSF ADVANTSLAI TWKGPPDWTD YNDFELQWFP
1310 1320 1330 1340 1350
GDALTIFNPY SSRKSEGRIV YGLHPGRSYQ FSVKTVSGDS WKTYSKPISG
1360 1370 1380 1390 1400
SVRTKPDKIQ NLHCRPQNST AIACSWIPPD SDFDGYSIEC RKMDTQEIEF
1410 1420 1430 1440 1450
SRKLEKEKSL LNIMMLVPHK RYLVSIKVQS AGMTSEVVED STITMIDRPP
1460 1470 1480 1490 1500
QPPPHIRVNE KDVLISKSSI NFTVNCSWFS DTNGAVKYFA VVVREADSMD
1510 1520 1530 1540 1550
ELKPEQQHPL PSYLEYRHNA SIRVYQTNYF ASKCAESPDS SSKSFNIKLG
1560 1570 1580 1590 1600
AEMDSLGGKC DPSQQKFCDG PLKPHTAYRI SIRAFTQLFD EDLKEFTKPL
1610 1620 1630 1640 1650
YSDTFFSMPI TTESEPLFGV IEGVSAGLFL IGMLVALVAF FICRQKASHS
1660 1670 1680 1690 1700
RERPSARLSI RRDRPLSVHL NLGQKGNRKT SCPIKINQFE GHFMKLQADS
1710 1720 1730 1740 1750
NYLLSKEYED LKDVGRSQSC DIALLPENRG KNRYNNILPY DASRVKLCNV
1760 1770 1780 1790 1800
DDDPCSDYIN ASYIPGNNFR REYIATQGPL PGTKDDFWKM AWEQNVHNIV
1810 1820 1830 1840 1850
MVTQCVEKGR VKCDHYWPAD QDPLYYGDLI LQMVSESVLP EWTIREFKIC
1860 1870 1880 1890 1900
SEEQLDAHRL IRHFHYTVWP DHGVPETTQS LIQFVRTVRD YINRSPGAGP
1910 1920 1930 1940 1950
TVVHCSAGVG RTGTFVALDR ILQQLDSKDS VDIYGAVHDL RLHRVHMVQT
1960 1970 1980 1990
ECQYVYLHQC VRDVLRAKKL RNEQENPLFP IYENVNPEYH RDAIYSRH
Length:1,998
Mass (Da):224,495
Last modified:July 1, 2008 - v1
Checksum:i066EE7141F942887
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P7W3A0A1W2P7W3_MOUSE
Protein tyrosine phosphatase, recep...
Ptprb mCG_125055
2,285Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti647V → A in BAE28060 (PubMed:16141072).Curated1
Sequence conflicti647V → A in BAE27912 (PubMed:16141072).Curated1
Sequence conflicti906T → P in AAL75813 (PubMed:12234928).Curated1
Sequence conflicti1037L → F in BAE28060 (PubMed:16141072).Curated1
Sequence conflicti1037L → F in BAE27912 (PubMed:16141072).Curated1
Sequence conflicti1168I → V in BAE28060 (PubMed:16141072).Curated1
Sequence conflicti1168I → V in BAE27912 (PubMed:16141072).Curated1
Sequence conflicti1748C → S in AAL75813 (PubMed:12234928).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY077755 mRNA Translation: AAL75813.1
AK147439 mRNA Translation: BAE27912.1
AK147668 mRNA Translation: BAE28060.1
CH466539 Genomic DNA Translation: EDL21786.1
BC141006 mRNA Translation: AAI41007.1
BC145111 mRNA Translation: AAI45112.1
CCDSiCCDS36063.1
RefSeqiNP_084204.2, NM_029928.2
UniGeneiMm.37213
Mm.489025

Genome annotation databases

EnsembliENSMUST00000092167; ENSMUSP00000089805; ENSMUSG00000020154
GeneIDi19263
KEGGimmu:19263
UCSCiuc007hbv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY077755 mRNA Translation: AAL75813.1
AK147439 mRNA Translation: BAE27912.1
AK147668 mRNA Translation: BAE28060.1
CH466539 Genomic DNA Translation: EDL21786.1
BC141006 mRNA Translation: AAI41007.1
BC145111 mRNA Translation: AAI45112.1
CCDSiCCDS36063.1
RefSeqiNP_084204.2, NM_029928.2
UniGeneiMm.37213
Mm.489025

3D structure databases

ProteinModelPortaliB2RU80
SMRiB2RU80
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiB2RU80, 2 interactors
MINTiB2RU80
STRINGi10090.ENSMUSP00000089805

PTM databases

iPTMnetiB2RU80
PhosphoSitePlusiB2RU80

Proteomic databases

MaxQBiB2RU80
PaxDbiB2RU80
PeptideAtlasiB2RU80
PRIDEiB2RU80

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092167; ENSMUSP00000089805; ENSMUSG00000020154
GeneIDi19263
KEGGimmu:19263
UCSCiuc007hbv.2 mouse

Organism-specific databases

CTDi5787
MGIiMGI:97809 Ptprb

Phylogenomic databases

eggNOGiKOG0791 Eukaryota
COG5599 LUCA
GeneTreeiENSGT00930000150847
HOGENOMiHOG000060224
HOVERGENiHBG053759
InParanoidiB2RU80
KOiK05694
OMAiPHIRVNK
OrthoDBiEOG091G00JF
PhylomeDBiB2RU80
TreeFamiTF351926

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiPtprb mouse
PROiPR:B2RU80
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020154 Expressed in 245 organ(s), highest expression level in lung
ExpressionAtlasiB2RU80 baseline and differential
GenevisibleiB2RU80 MM

Family and domain databases

CDDicd00063 FN3, 12 hits
Gene3Di2.60.40.10, 15 hits
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR029021 Prot-tyrosine_phosphatase-like
IPR000242 PTPase_domain
IPR016130 Tyr_Pase_AS
IPR003595 Tyr_Pase_cat
IPR000387 TYR_PHOSPHATASE_dom
PfamiView protein in Pfam
PF00041 fn3, 15 hits
PF00102 Y_phosphatase, 1 hit
PRINTSiPR00700 PRTYPHPHTASE
SMARTiView protein in SMART
SM00060 FN3, 16 hits
SM00194 PTPc, 1 hit
SM00404 PTPc_motif, 1 hit
SUPFAMiSSF49265 SSF49265, 16 hits
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 12 hits
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50055 TYR_PHOSPHATASE_PTP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPTPRB_MOUSE
AccessioniPrimary (citable) accession number: B2RU80
Secondary accession number(s): Q3UGZ7, Q8CIW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 1, 2008
Last modified: November 7, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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