Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - B2RU80 (PTPRB_MOUSE)

Protein

Receptor-type tyrosine-protein phosphatase beta

Gene

Ptprb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells and this requires the presence of plakoglobin.6 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1871SubstrateBy similarity1
Active sitei1905Phosphocysteine intermediatePROSITE-ProRule annotation1
Binding sitei1949SubstrateBy similarity1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

  • angiogenesis Source: UniProtKB
  • dephosphorylation Source: UniProtKB
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processAngiogenesis

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase beta (EC:3.1.3.48)
Short name:
Protein-tyrosine phosphatase beta
Short name:
R-PTP-beta
Alternative name(s):
Vascular endothelial protein tyrosine phosphatase
Short name:
VE-PTP
Gene namesi
Name:Ptprb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97809 Ptprb

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 1622ExtracellularSequence analysisAdd BLAST1600
Transmembranei1623 – 1643HelicalSequence analysisAdd BLAST21
Topological domaini1644 – 1997CytoplasmicSequence analysisAdd BLAST354

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show severe cardiovascular defects and embryonic lethality by E10. Vasculogenesis occurs normally however, angiogenesis is abnormal. Angiogenic defects are most pronounced in the yolk sac and include a complete failure to elaborate the primitive vascular scaffold into higher-order branched arteries, veins, and capillaries.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1911R → A: Loss of activity and dephosphorylation of CDH5. 1 Publication1
Mutagenesisi1982Y → F: Loss of tyrosine phosphorylation. Abolishes interaction with FYN and GRB2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000039040123 – 1998Receptor-type tyrosine-protein phosphatase betaAdd BLAST1976

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi53N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi173N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi199N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi268N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi415N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi422N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi480N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi575N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi599N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi653N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi830N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1041N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1097N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1164N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1186N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1213N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1275N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1368N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1471N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1475N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1519N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei1982Phosphotyrosine1 Publication1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiB2RU80
PaxDbiB2RU80
PeptideAtlasiB2RU80
PRIDEiB2RU80

PTM databases

iPTMnetiB2RU80
PhosphoSitePlusiB2RU80

Expressioni

Tissue specificityi

Expression is very high in the vasculature of lung, spleen, and kidney, as well as in the heart valves, and is also present in the endothelium of arterioles and venules. Also expressed in tumor vasculature.3 Publications

Developmental stagei

Expressed in both arterial and venous vascular endothelium in embryos, although more strongly in arterial vessels. Highly expressed in the developing outflow tract of the heart and later is expressed in developing heart valves.3 Publications

Gene expression databases

BgeeiENSMUSG00000020154 Expressed in 245 organ(s), highest expression level in lung
ExpressionAtlasiB2RU80 baseline and differential
GenevisibleiB2RU80 MM

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with TEK (PubMed:10557082, PubMed:19451274). Interacts via fibronectin type-III 17 domain with CDH5 (PubMed:19015309). Detected in a complex with CNTN1 and NRCAM (PubMed:11564762). Interacts (phosphorylated form) with FYN and GRB2 (PubMed:20398064).By similarity6 Publications

Protein-protein interaction databases

IntActiB2RU80, 2 interactors
MINTiB2RU80
STRINGi10090.ENSMUSP00000089805

Structurei

3D structure databases

ProteinModelPortaliB2RU80
SMRiB2RU80
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 109Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST87
Domaini113 – 206Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST94
Domaini207 – 291Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST85
Domaini292 – 384Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST93
Domaini378 – 466Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST89
Domaini470 – 556Fibronectin type-III 6PROSITE-ProRule annotationAdd BLAST87
Domaini557 – 642Fibronectin type-III 7PROSITE-ProRule annotationAdd BLAST86
Domaini643 – 733Fibronectin type-III 8PROSITE-ProRule annotationAdd BLAST91
Domaini734 – 821Fibronectin type-III 9PROSITE-ProRule annotationAdd BLAST88
Domaini822 – 913Fibronectin type-III 10PROSITE-ProRule annotationAdd BLAST92
Domaini908 – 994Fibronectin type-III 11PROSITE-ProRule annotationAdd BLAST87
Domaini995 – 1088Fibronectin type-III 12PROSITE-ProRule annotationAdd BLAST94
Domaini1086 – 1173Fibronectin type-III 13PROSITE-ProRule annotationAdd BLAST88
Domaini1176 – 1263Fibronectin type-III 14PROSITE-ProRule annotationAdd BLAST88
Domaini1264 – 1357Fibronectin type-III 15PROSITE-ProRule annotationAdd BLAST94
Domaini1358 – 1449Fibronectin type-III 16PROSITE-ProRule annotationAdd BLAST92
Domaini1449 – 1551Fibronectin type-III 17PROSITE-ProRule annotationAdd BLAST103
Domaini1704 – 1964Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST261

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1905 – 1911Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0791 Eukaryota
COG5599 LUCA
GeneTreeiENSGT00930000150847
HOGENOMiHOG000060224
HOVERGENiHBG053759
InParanoidiB2RU80
KOiK05694
OMAiPHIRVNK
OrthoDBiEOG091G00JF
PhylomeDBiB2RU80
TreeFamiTF351926

Family and domain databases

CDDicd00063 FN3, 12 hits
Gene3Di2.60.40.10, 15 hits
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR029021 Prot-tyrosine_phosphatase-like
IPR000242 PTPase_domain
IPR016130 Tyr_Pase_AS
IPR003595 Tyr_Pase_cat
IPR000387 TYR_PHOSPHATASE_dom
PfamiView protein in Pfam
PF00041 fn3, 15 hits
PF00102 Y_phosphatase, 1 hit
PRINTSiPR00700 PRTYPHPHTASE
SMARTiView protein in SMART
SM00060 FN3, 16 hits
SM00194 PTPc, 1 hit
SM00404 PTPc_motif, 1 hit
SUPFAMiSSF49265 SSF49265, 16 hits
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 12 hits
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50055 TYR_PHOSPHATASE_PTP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

B2RU80-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRHGALTAL WITLSVVQTG VAEQVKCNFT LLESRVSSLS ASIQWRTFAS 
        60         70         80         90        100
PCNFSLIYSS DTSGPMWCHP IRIDNFTYGC NPKDLQAGTV YNFRIVSLDG 
       110        120        130        140        150
EESTLVLQTD PLPPARFEVN REKTASTTLQ VRWTPSSGKV SWYEVQLFDH 
       160        170        180        190        200
NNQKIQEVQV QESTTWSQYT FLNLTEGNSY KVAITAVSGE KRSFPVYING 
       210        220        230        240        250
STVPSPVKDL GISPNPNSLL ISWSRGSGNV EQYRLVLMDK GAIVQDTNVD 
       260        270        280        290        300
RRDTSYAFHE LTPGHLYNLT IVTMASGLQN SRWKLVRTAP MEVSNLKVTN 
       310        320        330        340        350
DGRLTSLNVK WQKPPGDVDS YSITLSHQGT IKESKTLAPP VTETQFKDLV 
       360        370        380        390        400
PGRLYQVTIS CISGELSAEK SAAGRTVPEK VRNLVSYNEI WMKSFTVNWT 
       410        420        430        440        450
PPAGDWEHYR IVLFNESLVL LNTTVGKEET HYALDGLELI PGRQYEIEVI 
       460        470        480        490        500
VESGNLRNSE RCQGRTVPLA VLQLRVKHAN ETSLGITWRA PLGEWEKYII 
       510        520        530        540        550
SLMDRELLVI HKSLSKDAKE FTFTDLMPGR NYKATVTSMS GDLKQSSSIK 
       560        570        580        590        600
GRTVPAQVTD LHVNNQGMTS SLFTNWTKAL GDVEFYQVLL IHENVVVKNE 
       610        620        630        640        650
SVSSDTSRYS FRALKPGSLY SVVVTTVSGG ISSRQVVAEG RTVPSSVSGV 
       660        670        680        690        700
TVNNSGRNDY LSVSWLPAPG EVDHYVVSLS HEGKVDQFLI IAKSVSECSF 
       710        720        730        740        750
SSLTPGRLYN VTVTTKSGNY ASHSFTEERT VPDKVQGISV SNSARSDYLK 
       760        770        780        790        800
VSWVHATGDF DHYEVTIKNR ESFIQTKTIP KSENECEFIE LVPGRLYSVT 
       810        820        830        840        850
VSTKSGQYEA SEQGTGRTIP EPVKDLTLLN RSTEDLHVTW SRANGDVDQY 
       860        870        880        890        900
EVQLLFNDMK VFPHIHLVNT ATEYKFTALT PGRHYKILVL TISGDVQQSA 
       910        920        930        940        950
FIEGLTVPST VKNIHISANG ATDRLMVTWS PGGGDVDSYV VSAFRQDEKV 
       960        970        980        990       1000
DSQTIPKHAS EHTFHRLEAG AKYRIAIVSV SGSLRNQIDA LGQTVPASVQ 
      1010       1020       1030       1040       1050
GVVAANAYSS NSLTVSWQKA LGVAERYDIL LLNENGLLLS NVSEPATARQ 
      1060       1070       1080       1090       1100
HKFEDLTPGK KYKMQILTVS GGLFSKESQA EGRTVPAAVT NLRITENSSR 
      1110       1120       1130       1140       1150
YLSFGWTASE GELSWYNIFL YNPDRTLQER AQVDPLVQSF SFQNLLQGRM 
      1160       1170       1180       1190       1200
YKMVIVTHSG ELSNESFIFG RTVPAAVNHL KGSHRNTTDS LWFSWSPASG 
      1210       1220       1230       1240       1250
DFDFYELILY NPNGTKKENW KEKDVTEWRF QGLVPGRKYT LYVVTHSGDL 
      1260       1270       1280       1290       1300
SNKVTGEGRT APSPPSLLSF ADVANTSLAI TWKGPPDWTD YNDFELQWFP 
      1310       1320       1330       1340       1350
GDALTIFNPY SSRKSEGRIV YGLHPGRSYQ FSVKTVSGDS WKTYSKPISG 
      1360       1370       1380       1390       1400
SVRTKPDKIQ NLHCRPQNST AIACSWIPPD SDFDGYSIEC RKMDTQEIEF 
      1410       1420       1430       1440       1450
SRKLEKEKSL LNIMMLVPHK RYLVSIKVQS AGMTSEVVED STITMIDRPP 
      1460       1470       1480       1490       1500
QPPPHIRVNE KDVLISKSSI NFTVNCSWFS DTNGAVKYFA VVVREADSMD 
      1510       1520       1530       1540       1550
ELKPEQQHPL PSYLEYRHNA SIRVYQTNYF ASKCAESPDS SSKSFNIKLG 
      1560       1570       1580       1590       1600
AEMDSLGGKC DPSQQKFCDG PLKPHTAYRI SIRAFTQLFD EDLKEFTKPL 
      1610       1620       1630       1640       1650
YSDTFFSMPI TTESEPLFGV IEGVSAGLFL IGMLVALVAF FICRQKASHS 
      1660       1670       1680       1690       1700
RERPSARLSI RRDRPLSVHL NLGQKGNRKT SCPIKINQFE GHFMKLQADS 
      1710       1720       1730       1740       1750
NYLLSKEYED LKDVGRSQSC DIALLPENRG KNRYNNILPY DASRVKLCNV 
      1760       1770       1780       1790       1800
DDDPCSDYIN ASYIPGNNFR REYIATQGPL PGTKDDFWKM AWEQNVHNIV 
      1810       1820       1830       1840       1850
MVTQCVEKGR VKCDHYWPAD QDPLYYGDLI LQMVSESVLP EWTIREFKIC 
      1860       1870       1880       1890       1900
SEEQLDAHRL IRHFHYTVWP DHGVPETTQS LIQFVRTVRD YINRSPGAGP 
      1910       1920       1930       1940       1950
TVVHCSAGVG RTGTFVALDR ILQQLDSKDS VDIYGAVHDL RLHRVHMVQT 
      1960       1970       1980       1990 
ECQYVYLHQC VRDVLRAKKL RNEQENPLFP IYENVNPEYH RDAIYSRH
Length:1,998
Mass (Da):224,495
Last modified:July 1, 2008 - v1
Checksum:i066EE7141F942887
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P7W3A0A1W2P7W3_MOUSE
Protein tyrosine phosphatase, recep...
Ptprb mCG_125055
2,285Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti647V → A in BAE28060 (PubMed:16141072).Curated1
Sequence conflicti647V → A in BAE27912 (PubMed:16141072).Curated1
Sequence conflicti906T → P in AAL75813 (PubMed:12234928).Curated1
Sequence conflicti1037L → F in BAE28060 (PubMed:16141072).Curated1
Sequence conflicti1037L → F in BAE27912 (PubMed:16141072).Curated1
Sequence conflicti1168I → V in BAE28060 (PubMed:16141072).Curated1
Sequence conflicti1168I → V in BAE27912 (PubMed:16141072).Curated1
Sequence conflicti1748C → S in AAL75813 (PubMed:12234928).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY077755 mRNA Translation: AAL75813.1
AK147439 mRNA Translation: BAE27912.1
AK147668 mRNA Translation: BAE28060.1
CH466539 Genomic DNA Translation: EDL21786.1
BC141006 mRNA Translation: AAI41007.1
BC145111 mRNA Translation: AAI45112.1
CCDSiCCDS36063.1
RefSeqiNP_084204.2, NM_029928.2
UniGeneiMm.37213
Mm.489025

Genome annotation databases

EnsembliENSMUST00000092167; ENSMUSP00000089805; ENSMUSG00000020154
GeneIDi19263
KEGGimmu:19263
UCSCiuc007hbv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY077755 mRNA Translation: AAL75813.1
AK147439 mRNA Translation: BAE27912.1
AK147668 mRNA Translation: BAE28060.1
CH466539 Genomic DNA Translation: EDL21786.1
BC141006 mRNA Translation: AAI41007.1
BC145111 mRNA Translation: AAI45112.1
CCDSiCCDS36063.1
RefSeqiNP_084204.2, NM_029928.2
UniGeneiMm.37213
Mm.489025

3D structure databases

ProteinModelPortaliB2RU80
SMRiB2RU80
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiB2RU80, 2 interactors
MINTiB2RU80
STRINGi10090.ENSMUSP00000089805

PTM databases

iPTMnetiB2RU80
PhosphoSitePlusiB2RU80

Proteomic databases

MaxQBiB2RU80
PaxDbiB2RU80
PeptideAtlasiB2RU80
PRIDEiB2RU80

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092167; ENSMUSP00000089805; ENSMUSG00000020154
GeneIDi19263
KEGGimmu:19263
UCSCiuc007hbv.2 mouse

Organism-specific databases

CTDi5787
MGIiMGI:97809 Ptprb

Phylogenomic databases

eggNOGiKOG0791 Eukaryota
COG5599 LUCA
GeneTreeiENSGT00930000150847
HOGENOMiHOG000060224
HOVERGENiHBG053759
InParanoidiB2RU80
KOiK05694
OMAiPHIRVNK
OrthoDBiEOG091G00JF
PhylomeDBiB2RU80
TreeFamiTF351926

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiPtprb mouse
PROiPR:B2RU80
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020154 Expressed in 245 organ(s), highest expression level in lung
ExpressionAtlasiB2RU80 baseline and differential
GenevisibleiB2RU80 MM

Family and domain databases

CDDicd00063 FN3, 12 hits
Gene3Di2.60.40.10, 15 hits
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR029021 Prot-tyrosine_phosphatase-like
IPR000242 PTPase_domain
IPR016130 Tyr_Pase_AS
IPR003595 Tyr_Pase_cat
IPR000387 TYR_PHOSPHATASE_dom
PfamiView protein in Pfam
PF00041 fn3, 15 hits
PF00102 Y_phosphatase, 1 hit
PRINTSiPR00700 PRTYPHPHTASE
SMARTiView protein in SMART
SM00060 FN3, 16 hits
SM00194 PTPc, 1 hit
SM00404 PTPc_motif, 1 hit
SUPFAMiSSF49265 SSF49265, 16 hits
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 12 hits
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50055 TYR_PHOSPHATASE_PTP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPTPRB_MOUSE
AccessioniPrimary (citable) accession number: B2RU80
Secondary accession number(s): Q3UGZ7, Q8CIW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 1, 2008
Last modified: November 7, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again