UniProtKB - B2RSH2 (GNAI1_MOUSE)
Guanine nucleotide-binding protein G(i) subunit alpha-1
Gnai1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | MagnesiumBy similarity | 1 | |
Metal bindingi | 181 | MagnesiumBy similarity | 1 | |
Binding sitei | 326 | GTP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 43 – 48 | GTPBy similarity | 6 | |
Nucleotide bindingi | 150 – 151 | GTPBy similarity | 2 | |
Nucleotide bindingi | 175 – 178 | GTPBy similarity | 4 | |
Nucleotide bindingi | 200 – 204 | GTPBy similarity | 5 | |
Nucleotide bindingi | 269 – 272 | GTPBy similarity | 4 |
GO - Molecular functioni
- GDP binding Source: UniProtKB
- G-protein beta/gamma-subunit complex binding Source: GO_Central
- G protein-coupled receptor binding Source: UniProtKB
- G protein-coupled serotonin receptor binding Source: MGI
- GTPase activating protein binding Source: MGI
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
GO - Biological processi
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB
- cellular response to forskolin Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: UniProtKB
- negative regulation of synaptic transmission Source: MGI
- positive regulation of protein localization to cell cortex Source: UniProtKB
- regulation of cAMP-mediated signaling Source: UniProtKB
- regulation of mitotic spindle organization Source: UniProtKB
Keywordsi
Molecular function | Transducer |
Biological process | Cell cycle, Cell division, Mitosis, Transport |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-112043, PLC beta mediated events R-MMU-170670, Adenylate cyclase inhibitory pathway R-MMU-202040, G-protein activation R-MMU-392170, ADP signalling through P2Y purinoceptor 12 R-MMU-400042, Adrenaline,noradrenaline inhibits insulin secretion R-MMU-418594, G alpha (i) signalling events R-MMU-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-MMU-9009391, Extra-nuclear estrogen signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein G(i) subunit alpha-1Alternative name(s): Adenylate cyclase-inhibiting G alpha protein |
Gene namesi | Name:Gnai1 Synonyms:Gnai-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95771, Gnai1 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm By similarity
- cell cortex By similarity
- Membrane By similarity; Lipid-anchor By similarity
Note: Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody. Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane.By similarity
Cytoskeleton
- centrosome Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- heterotrimeric G-protein complex Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
- cell cortex region Source: UniProtKB
- cytoplasm Source: UniProtKB
- membrane Source: MGI
- membrane raft Source: MGI
- midbody Source: UniProtKB
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000408477 | 2 – 354 | Guanine nucleotide-binding protein G(i) subunit alpha-1Add BLAST | 353 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Lipidationi | 3 | S-palmitoyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, PalmitateProteomic databases
jPOSTi | B2RSH2 |
MaxQBi | B2RSH2 |
PaxDbi | B2RSH2 |
PeptideAtlasi | B2RSH2 |
PRIDEi | B2RSH2 |
PTM databases
iPTMneti | B2RSH2 |
PhosphoSitePlusi | B2RSH2 |
SwissPalmi | B2RSH2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000057614, Expressed in CA1 field of hippocampus and 292 other tissues |
Genevisiblei | B2RSH2, MM |
Interactioni
Subunit structurei
Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Part of a spindle orientation complex at least composed of GNAI1, GPSM2 and NUMA1. Identified in complex with the beta subunit GNB1 and the gamma subunit GNG1. Identified in complex with the beta subunit GNB1 and the gamma subunit GNG2. GTP binding causes dissociation of the heterotrimer, liberating the individual subunits so that they can interact with downstream effector proteins (By similarity).
Interacts (GDP-bound form) with GPSM1; this inhibits guanine nucleotide exchange and GTP binding (By similarity).
Interacts (GDP-bound form) with GPSM2 (via GoLoco domains); this inhibits guanine nucleotide exchange.
Interacts with RGS10; this strongly enhances GTP hydrolysis.
Interacts with RGS1 and RGS16; this strongly enhances GTPase activity.
Interacts with RGS4.
Interacts with RGS12.
Interacts (via active GTP- or inactive GDP-bound forms) with RGS14 (via RGS and GoLoco domains).
Interacts with RGS3, RGS6, RGS7, RGS8, RGS17, RGS18 and RGS20 (in vitro) (By similarity).
Interacts (GDP-bound form) with RIC8A (via C-terminus) (By similarity).
Interacts (inactive GDP-bound form) with NUCB1 (via GBA motif); the interaction leads to activation of GNAI1 (By similarity).
Interacts (inactive GDP-bound form) with CCDC88C/DAPLE (via GBA motif); the interaction leads to activation of GNAI1 (By similarity).
Interacts (inactive GDP-bound form) with CCDC8A/GIV (via GBA motif) (By similarity).
By similarityGO - Molecular functioni
- G protein-coupled receptor binding Source: UniProtKB
- G protein-coupled serotonin receptor binding Source: MGI
- GTPase activating protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199966, 23 interactors |
CORUMi | B2RSH2 |
IntActi | B2RSH2, 3 interactors |
STRINGi | 10090.ENSMUSP00000074259 |
Miscellaneous databases
RNActi | B2RSH2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 354 | G-alphaPROSITE-ProRule annotationAdd BLAST | 323 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 48 | G1 motifPROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 173 – 181 | G2 motifPROSITE-ProRule annotation | 9 | |
Regioni | 196 – 205 | G3 motifPROSITE-ProRule annotation | 10 | |
Regioni | 265 – 272 | G4 motifPROSITE-ProRule annotation | 8 | |
Regioni | 324 – 329 | G5 motifPROSITE-ProRule annotation | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000153567 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | B2RSH2 |
OMAi | RIAQTSY |
OrthoDBi | 754573at2759 |
PhylomeDBi | B2RSH2 |
TreeFami | TF300673 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGCTLSAEDK AAVERSKMID RNLREDGEKA AREVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEAG YSEEECKQYK AVVYSNTIQS IIAIIRAMGR LKIDFGDSAR
110 120 130 140 150
ADDARQLFVL AGAAEEGFMT AELAGVIKRL WKDSGVQACF NRSREYQLND
160 170 180 190 200
SAAYYLNDLD RIAQPNYIPT QQDVLRTRVK TTGIVETHFT FKDLHFKMFD
210 220 230 240 250
VGGQRSERKK WIHCFEGVTA IIFCVALSDY DLVLAEDEEM NRMHESMKLF
260 270 280 290 300
DSICNNKWFT DTSIILFLNK KDLFEEKIKK SPLTICYPEY AGSNTYEEAA
310 320 330 340 350
AYIQCQFEDL NKRKDTKEIY THFTCATDTK NVQFVFDAVT DVIIKNNLKD
CGLF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC138862 mRNA Translation: AAI38863.1 BC138865 mRNA Translation: AAI38866.1 |
CCDSi | CCDS39018.1 |
RefSeqi | NP_034435.1, NM_010305.1 |
Genome annotation databases
Ensembli | ENSMUST00000074694; ENSMUSP00000074259; ENSMUSG00000057614 |
GeneIDi | 14677 |
KEGGi | mmu:14677 |
UCSCi | uc008wns.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC138862 mRNA Translation: AAI38863.1 BC138865 mRNA Translation: AAI38866.1 |
CCDSi | CCDS39018.1 |
RefSeqi | NP_034435.1, NM_010305.1 |
3D structure databases
SMRi | B2RSH2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199966, 23 interactors |
CORUMi | B2RSH2 |
IntActi | B2RSH2, 3 interactors |
STRINGi | 10090.ENSMUSP00000074259 |
PTM databases
iPTMneti | B2RSH2 |
PhosphoSitePlusi | B2RSH2 |
SwissPalmi | B2RSH2 |
Proteomic databases
jPOSTi | B2RSH2 |
MaxQBi | B2RSH2 |
PaxDbi | B2RSH2 |
PeptideAtlasi | B2RSH2 |
PRIDEi | B2RSH2 |
Protocols and materials databases
Antibodypediai | 15099, 293 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000074694; ENSMUSP00000074259; ENSMUSG00000057614 |
GeneIDi | 14677 |
KEGGi | mmu:14677 |
UCSCi | uc008wns.1, mouse |
Organism-specific databases
CTDi | 2770 |
MGIi | MGI:95771, Gnai1 |
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000153567 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | B2RSH2 |
OMAi | RIAQTSY |
OrthoDBi | 754573at2759 |
PhylomeDBi | B2RSH2 |
TreeFami | TF300673 |
Enzyme and pathway databases
Reactomei | R-MMU-112043, PLC beta mediated events R-MMU-170670, Adenylate cyclase inhibitory pathway R-MMU-202040, G-protein activation R-MMU-392170, ADP signalling through P2Y purinoceptor 12 R-MMU-400042, Adrenaline,noradrenaline inhibits insulin secretion R-MMU-418594, G alpha (i) signalling events R-MMU-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-MMU-9009391, Extra-nuclear estrogen signaling |
Miscellaneous databases
BioGRID-ORCSi | 14677, 0 hits in 18 CRISPR screens |
ChiTaRSi | Gnai1, mouse |
PROi | PR:B2RSH2 |
RNActi | B2RSH2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000057614, Expressed in CA1 field of hippocampus and 292 other tissues |
Genevisiblei | B2RSH2, MM |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNAI1_MOUSE | |
Accessioni | B2RSH2Primary (citable) accession number: B2RSH2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 31, 2011 |
Last sequence update: | July 1, 2008 | |
Last modified: | December 2, 2020 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families