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Protein

OTU domain-containing protein 4

Gene

Otud4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein. May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators (PubMed:29395066). Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase-substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions (By similarity).By similarity1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Activity regulationi

Phosphorylation on Ser-202 and Ser-204 induces 'Lys-63'-specific deubiquitinase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei42By similarity1
Active sitei45Nucleophile1 Publication1
Active sitei148By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processImmunity, Innate immunity, Ubl conjugation pathway

Protein family/group databases

MEROPSiC85.002

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 4By similarity (EC:3.4.19.121 Publication)
Gene namesi
Name:Otud4Imported
Synonyms:Kiaa10461 PublicationImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1098801 Otud4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are born at sub-Mendelian rate.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45C → A: Abolishes 'Lys-48'- and 'Lys-63'-specific deubiquitinase activity. Impairs 'Lys-63'-specific deubiquitination of TRAF6 substrate. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003944581 – 1107OTU domain-containing protein 4Add BLAST1107

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei120PhosphotyrosineBy similarity1
Modified residuei126PhosphoserineBy similarity1
Modified residuei128PhosphoserineBy similarity1
Modified residuei131PhosphothreonineBy similarity1
Modified residuei166PhosphoserineBy similarity1
Modified residuei199PhosphoserineBy similarity1
Modified residuei202Phosphoserine1 Publication1
Modified residuei204PhosphoserineBy similarity1
Modified residuei340PhosphoserineBy similarity1
Modified residuei438PhosphotyrosineCombined sources1
Modified residuei442PhosphoserineCombined sources1
Modified residuei459PhosphotyrosineBy similarity1
Modified residuei544PhosphoserineBy similarity1
Modified residuei895PhosphoserineBy similarity1
Modified residuei1000PhosphoserineCombined sources1
Modified residuei1005PhosphoserineCombined sources1
Modified residuei1016PhosphoserineCombined sources1
Modified residuei1017PhosphoserineCombined sources1
Modified residuei1042PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-202 and Ser-204 activates 'Lys-63'-specific deubiquitinase activity. Induced upon stimulation with IL1B.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiB2RRE7
MaxQBiB2RRE7
PaxDbiB2RRE7
PeptideAtlasiB2RRE7
PRIDEiB2RRE7

PTM databases

iPTMnetiB2RRE7
PhosphoSitePlusiB2RRE7

Expressioni

Gene expression databases

BgeeiENSMUSG00000036990 Expressed in 289 organ(s), highest expression level in secondary oocyte
ExpressionAtlasiB2RRE7 baseline and differential
GenevisibleiB2RRE7 MM

Interactioni

Subunit structurei

Interacts with MYD88; the interaction is direct (PubMed:29395066). Interacts with ALKBH3; the interaction is direct. Interacts with USP7; the interaction is direct. Interacts with USP9X; the interaction is direct (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi216375, 4 interactors
IntActiB2RRE7, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliB2RRE7
SMRiB2RRE7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 155OTUPROSITE-ProRule annotationAdd BLAST122

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni39 – 45Cys-loopBy similarity7
Regioni94 – 104Variable-loopBy similarityAdd BLAST11
Regioni143 – 148His-loopBy similarity6

Phylogenomic databases

eggNOGiENOG410INQF Eukaryota
ENOG410ZCAJ LUCA
GeneTreeiENSGT00530000063508
HOGENOMiHOG000168383
HOVERGENiHBG082091
InParanoidiB2RRE7
KOiK13718
OMAiTEASVNG
OrthoDBiEOG091G00ZF
PhylomeDBiB2RRE7
TreeFamiTF326812

Family and domain databases

InterProiView protein in InterPro
IPR003323 OTU_dom
IPR038765 Papain_like_cys_pep_sf
PfamiView protein in Pfam
PF02338 OTU, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50802 OTU, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

B2RRE7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEAAVGAPDG VDQGGVGPLE DETPMDAYLR KLGLYRKLVA KDGSCLFRAV
60 70 80 90 100
AEQVLHSQSR HVEVRMACIR YLRENREKFE AFIEGSFEEY LKRLENPQEW
110 120 130 140 150
VGQVEISALS LMYRKDFVIY QEPNVSPSHV TENNFPEKVL LCFSNGNHYD
160 170 180 190 200
IVYPITYKDS SAMCQSLLYE LLYEKVFKTD VSKIMMGLEA SEVAEESNSE
210 220 230 240 250
ISDSEDDSCK SKSTAATDVN GFKPSGSENP KNNGNSADLP LSRKVLKSLN
260 270 280 290 300
PAVYRNVEYE IWLKSKQAQQ KRDYSIAAGL QYEVGDKCHQ VRLDHNGKLS
310 320 330 340 350
NADIHGVHSE NGLVLSEELG KKHTPKNLKP PPPESWNTVS GKKMKKPNSG
360 370 380 390 400
QNFHSDTDYR GPKNLNKPIK APSALPPRLQ HPSSGVRQHA FSSHSTGSQS
410 420 430 440 450
QKSSSEHKNL SRMPSQITRK PDRERAEDFD HVSRESYYFG LSPEERREKQ
460 470 480 490 500
AIEESRLLYE IQNRDEQAFP ALSSSSVSQS PSQNSNACVP RKSSHARDRK
510 520 530 540 550
GSMRRADAEE RKDKDSLRGH THVDKKPEPS TLEISDDKCT RVSSPSKSKK
560 570 580 590 600
ECPSPVEQKP AEHIPLSNPA PLLVSPEVHL TPAVPSLPAT VPAWPSEPTT
610 620 630 640 650
FGPTGVPAQI PILSVTQTTG PDAAVSQAHL TPSPVPVSIQ AVNQPLMPLP
660 670 680 690 700
QTMSLYQDPL YPGFPCSEKG DRAIAPPYSL CQTGEDLPKD KNILRFFFNL
710 720 730 740 750
GVKAYSCPMW APHSYLYPLH QAYMAACRMY PKVPVPVYPQ NTWFQEAPPA
760 770 780 790 800
QSESDCPCTD AHYSLHPEAS VNGQMPQAEM GPPAFASPLV IPPSQVSEGH
810 820 830 840 850
GQLSYQPELE SENPGQLLHA EYEESLSGKN MYPQQSFGPN PFLGPVPIAP
860 870 880 890 900
PFFPHVWYGY PFQGFVENPV MRQNIVLPPD DKGELDLPLE NLDLSKECDS
910 920 930 940 950
VSAVDEFPDA RVEGAHSLSA ASVSSKHEGR VEQSSQTRKA DIDLASGSSA
960 970 980 990 1000
VEGKGHPPTQ ILNREREPGS AEPEPKRTIQ SLKEKPEKVK DPKTAADVVS
1010 1020 1030 1040 1050
PGANSVDRLQ RPKEESSEDE NEVSNILRSG RSKQFYNQTY GSRKYKSDWG
1060 1070 1080 1090 1100
SSGRGGYQHV RGEESWKGQP NRSRDEGYQY HRHVRGRPYR GDRRRSGMGD

GHRGQHT
Length:1,107
Mass (Da):123,055
Last modified:July 1, 2008 - v1
Checksum:i746B6BC8589FA672
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J260A0A0R4J260_MOUSE
OTU domain-containing protein 4
Otud4
1,106Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti290Missing in AAI45260 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC138373 mRNA Translation: AAI38374.1
BC145259 mRNA Translation: AAI45260.1
AK122429 mRNA Translation: BAC65711.1
AK015275 mRNA Translation: BAB29777.1
CCDSiCCDS57625.1
RefSeqiNP_001074633.1, NM_001081164.1
NP_001242962.1, NM_001256033.1
UniGeneiMm.34348

Genome annotation databases

EnsembliENSMUST00000173078; ENSMUSP00000133939; ENSMUSG00000036990
GeneIDi73945
KEGGimmu:73945
UCSCiuc009mir.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC138373 mRNA Translation: AAI38374.1
BC145259 mRNA Translation: AAI45260.1
AK122429 mRNA Translation: BAC65711.1
AK015275 mRNA Translation: BAB29777.1
CCDSiCCDS57625.1
RefSeqiNP_001074633.1, NM_001081164.1
NP_001242962.1, NM_001256033.1
UniGeneiMm.34348

3D structure databases

ProteinModelPortaliB2RRE7
SMRiB2RRE7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216375, 4 interactors
IntActiB2RRE7, 1 interactor

Protein family/group databases

MEROPSiC85.002

PTM databases

iPTMnetiB2RRE7
PhosphoSitePlusiB2RRE7

Proteomic databases

EPDiB2RRE7
MaxQBiB2RRE7
PaxDbiB2RRE7
PeptideAtlasiB2RRE7
PRIDEiB2RRE7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000173078; ENSMUSP00000133939; ENSMUSG00000036990
GeneIDi73945
KEGGimmu:73945
UCSCiuc009mir.1 mouse

Organism-specific databases

CTDi54726
MGIiMGI:1098801 Otud4
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410INQF Eukaryota
ENOG410ZCAJ LUCA
GeneTreeiENSGT00530000063508
HOGENOMiHOG000168383
HOVERGENiHBG082091
InParanoidiB2RRE7
KOiK13718
OMAiTEASVNG
OrthoDBiEOG091G00ZF
PhylomeDBiB2RRE7
TreeFamiTF326812

Miscellaneous databases

ChiTaRSiOtud4 mouse
PROiPR:B2RRE7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036990 Expressed in 289 organ(s), highest expression level in secondary oocyte
ExpressionAtlasiB2RRE7 baseline and differential
GenevisibleiB2RRE7 MM

Family and domain databases

InterProiView protein in InterPro
IPR003323 OTU_dom
IPR038765 Papain_like_cys_pep_sf
PfamiView protein in Pfam
PF02338 OTU, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50802 OTU, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOTUD4_MOUSE
AccessioniPrimary (citable) accession number: B2RRE7
Secondary accession number(s): B7ZNI5, Q80TL3, Q9CUN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: July 1, 2008
Last modified: November 7, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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