We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - B2JZ75 (AAS_YERPB)
Protein
Bifunctional protein Aas
Gene
aas
Organism
Yersinia pseudotuberculosis serotype IB (strain PB1/+)
Status
Functioni
Plays a role in lysophospholipid acylation. Transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3-phosphoethanolamine (2-acyl-GPE) formed by transacylation reactions or degradation by phospholipase A1.
UniRule annotationCatalytic activityi
- a 2-acyl-sn-glycero-3-phosphoethanolamine + a fatty acyl-[ACP] = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + holo-[ACP]UniRule annotationEC:2.3.1.40UniRule annotation
- a long-chain fatty acid + ATP + holo-[ACP] = a long-chain fatty acyl-[ACP] + AMP + diphosphateUniRule annotationEC:6.2.1.20UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 36 | UniRule annotation | 1 |
GO - Molecular functioni
- acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-KW
- long-chain fatty acid [acyl-carrier-protein] ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- fatty acid metabolic process Source: InterPro
- phospholipid biosynthetic process Source: InterPro
Keywordsi
Molecular function | Acyltransferase, Ligase, Multifunctional enzyme, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional protein AasUniRule annotationIncluding the following 2 domains: Alternative name(s): 2-acyl-GPE acyltransferaseUniRule annotation Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferaseUniRule annotation Alternative name(s): Acyl-ACP synthetaseUniRule annotation Long-chain-fatty-acid--[acyl-carrier-protein] ligaseUniRule annotation |
Gene namesi | Name:aasUniRule annotation Ordered Locus Names:YPTS_3164 |
Organismi | Yersinia pseudotuberculosis serotype IB (strain PB1/+) |
Taxonomic identifieri | 502801 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Yersiniaceae › Yersinia › |
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 258 – 277 | HelicalUniRule annotationAdd BLAST | 20 | |
Transmembranei | 409 – 433 | HelicalUniRule annotationAdd BLAST | 25 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000137904 | 1 – 718 | Bifunctional protein AasAdd BLAST | 718 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 15 – 138 | AcyltransferaseAdd BLAST | 124 | |
Regioni | 233 – 646 | AMP-bindingAdd BLAST | 414 |
Sequence similaritiesi
In the N-terminal section; belongs to the 2-acyl-GPE acetyltransferase family.UniRule annotation
In the C-terminal section; belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
OMAi | ANWVYLE |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
HAMAPi | MF_01162, Aas, 1 hit |
InterProi | View protein in InterPro IPR023775, Aas IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
B2JZ75-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAYRLLRALF RGLFRVTIDG VTDQFKHEKL IITPNHVSFL DGALLALFLP
60 70 80 90 100
IKPVFAVYTS ITDTWYMRWL KPYVDFVALD PTNPMAIKHL VRMVEQGRPV
110 120 130 140 150
VIFPEGRITV TGSLMKIYDG AAFVAAKSGA AVVPIRLDGP EFTHFGRLQG
160 170 180 190 200
VLKTRWFPKI SIHVLPATTI PMPQAPRSRE RRVLAGEHLH TIMMAARMAT
210 220 230 240 250
VPRETLFEAL LSAQTRYGRF KPCIEDVSFK EDSYQTLLKK TLGVSRILQR
260 270 280 290 300
FTVPGEHVGM LLPNATITAA AIFGASLRGR IPALLNYTSG AKGLQSAIIA
310 320 330 340 350
ASLKTIVTSR QFLEKGKLTH LPEQVNEVNW VYLEDLKDTV TLTDKLWILF
360 370 380 390 400
HLCFPRRAML PQQADDSALI LFTSGSEGNP KGVVHSHASL LANVEQIRTI
410 420 430 440 450
ADFTPRDRFM SSLPLFHAFG LTVGLFTPLM TGSRVFLYPS PLHYRVVPEL
460 470 480 490 500
VYDRNCTVLF GTSTFLGNYA RFAHPYDFAR VRYVVAGAEK LAESTKQIWQ
510 520 530 540 550
DKFGIRILEG YGVTECAPVV AINVPMAAKV NTVGRILPGM EARLINVPGI
560 570 580 590 600
AQGGRLQLRG PNIMRGYLRV ENPGVLEQPS AENAQGELDA NWYDTGDIVT
610 620 630 640 650
LDEQGFCAIR GRVKRFAKLA GEMVSLESVE QLAISLSPEG QHAAAAKTDS
660 670 680 690 700
AKGEALVLFT TDSEITRERL IKAARENGVP ELAVPRDIRV VKALPLLGSG
710
KPDFVTLGKM AQDPEMSV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001048 Genomic DNA Translation: ACC90119.1 |
RefSeqi | WP_011192876.1, NZ_CP009780.1 |
Genome annotation databases
GeneIDi | 66844526 |
KEGGi | ypb:YPTS_3164 |
PATRICi | fig|502801.10.peg.2597 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001048 Genomic DNA Translation: ACC90119.1 |
RefSeqi | WP_011192876.1, NZ_CP009780.1 |
3D structure databases
SMRi | B2JZ75 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 66844526 |
KEGGi | ypb:YPTS_3164 |
PATRICi | fig|502801.10.peg.2597 |
Phylogenomic databases
OMAi | ANWVYLE |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
HAMAPi | MF_01162, Aas, 1 hit |
InterProi | View protein in InterPro IPR023775, Aas IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AAS_YERPB | |
Accessioni | B2JZ75Primary (citable) accession number: B2JZ75 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 14, 2009 |
Last sequence update: | June 10, 2008 | |
Last modified: | February 23, 2022 | |
This is version 67 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |