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Protein

Ribulose bisphosphate carboxylase large chain

Gene

cbbL

Organism
Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) (Burkholderia phymatum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei141Substrate; in homodimeric partnerUniRule annotation1
Binding sitei191SubstrateUniRule annotation1
Active sitei193Proton acceptorUniRule annotation1
Binding sitei195SubstrateUniRule annotation1
Metal bindingi219Magnesium; via carbamate groupUniRule annotation1
Metal bindingi221MagnesiumUniRule annotation1
Metal bindingi222MagnesiumUniRule annotation1
Active sitei311Proton acceptorUniRule annotation1
Binding sitei312SubstrateUniRule annotation1
Binding sitei344SubstrateUniRule annotation1
Sitei351Transition state stabilizerUniRule annotation1
Binding sitei396SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, Monooxygenase, Oxidoreductase
Biological processCalvin cycle, Carbon dioxide fixation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciBPHY391038:G1GBS-6601-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:cbbLUniRule annotation
Ordered Locus Names:Bphy_6497
Encoded oniPlasmid pBPHY010 Publication
OrganismiParaburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) (Burkholderia phymatum)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeParaburkholderia
Proteomesi
  • UP000001192 Componenti: Plasmid pBPHY01

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003557481 – 501Ribulose bisphosphate carboxylase large chainAdd BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219N6-carboxylysineUniRule annotation1

Proteomic databases

PRIDEiB2JX50

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB2JX50
SMRiB2JX50
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000230831
KOiK01601
OMAiHRAMHAA
OrthoDBiPOG091H14UZ

Family and domain databases

CDDicd08212 RuBisCO_large_I, 1 hit
Gene3Di3.20.20.110, 1 hit
3.30.70.150, 1 hit
HAMAPiMF_01338 RuBisCO_L_type1, 1 hit
InterProiView protein in InterPro
IPR033966 RuBisCO
IPR020878 RuBisCo_large_chain_AS
IPR000685 RuBisCO_lsu_C
IPR036376 RuBisCO_lsu_C_sf
IPR017443 RuBisCO_lsu_fd_N
IPR036422 RuBisCO_lsu_N_sf
IPR020888 RuBisCO_lsuI
PANTHERiPTHR42704 PTHR42704, 1 hit
PfamiView protein in Pfam
PF00016 RuBisCO_large, 1 hit
PF02788 RuBisCO_large_N, 1 hit
SFLDiSFLDS00014 RuBisCO, 1 hit
SUPFAMiSSF51649 SSF51649, 1 hit
SSF54966 SSF54966, 1 hit
PROSITEiView protein in PROSITE
PS00157 RUBISCO_LARGE, 1 hit

Sequencei

Sequence statusi: Complete.

B2JX50-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNDFSKEAVK PADSATAAAK AEKRSRYAAG VMKYREMGYW QPDYTPKDTD
60 70 80 90 100
VIALFRITPQ PGVEPEEAAA AVAGESSTAT WTVVWTDRLT ACDMYRAKAF
110 120 130 140 150
RVEPVPNPAE GEPQYFAFIA YELDLFEEGS VANLTASIIG NVFGFKPLKA
160 170 180 190 200
LRLEDMRIPV AYLKTFQGPP TGIVVERERL DKYGRPLLGA TVKPKLGLSG
210 220 230 240 250
KNYGRVVYEG LKGGLDFLKD DENINSQPFM HWRDRYLFAM EAVHRAQAET
260 270 280 290 300
GEVKGHYLNV TAGTMEDMYE RAEFAKELGS CIVMIDLVIG WTAITSMGRW
310 320 330 340 350
ARKNDMILHL HRAGHGTYTR QRNHGISFRV IAKWLRMAGV DHAHAGTAVG
360 370 380 390 400
KLDGDPLSVQ GYYNVLRESH NSVDLTRGIF FDQHWAGLRK VMPVASGGIH
410 420 430 440 450
AGQMHQLLDL FGDDAILQFG GGTIGHPSGI QAGATANRVA LETMVKARNE
460 470 480 490 500
GRDIANEGSD LLEAAARHCT PLKQALDTWG DVTFNYTPTD SPDFAVTPSV

A
Length:501
Mass (Da):55,176
Last modified:June 10, 2008 - v1
Checksum:iE8238E2EFE685A2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001045 Genomic DNA Translation: ACC75527.1
RefSeqiWP_012405686.1, NC_010625.1

Genome annotation databases

EnsemblBacteriaiACC75527; ACC75527; Bphy_6497
GeneIDi27746061
KEGGibph:Bphy_6497

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001045 Genomic DNA Translation: ACC75527.1
RefSeqiWP_012405686.1, NC_010625.1

3D structure databases

ProteinModelPortaliB2JX50
SMRiB2JX50
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiB2JX50

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC75527; ACC75527; Bphy_6497
GeneIDi27746061
KEGGibph:Bphy_6497

Phylogenomic databases

HOGENOMiHOG000230831
KOiK01601
OMAiHRAMHAA
OrthoDBiPOG091H14UZ

Enzyme and pathway databases

BioCyciBPHY391038:G1GBS-6601-MONOMER

Family and domain databases

CDDicd08212 RuBisCO_large_I, 1 hit
Gene3Di3.20.20.110, 1 hit
3.30.70.150, 1 hit
HAMAPiMF_01338 RuBisCO_L_type1, 1 hit
InterProiView protein in InterPro
IPR033966 RuBisCO
IPR020878 RuBisCo_large_chain_AS
IPR000685 RuBisCO_lsu_C
IPR036376 RuBisCO_lsu_C_sf
IPR017443 RuBisCO_lsu_fd_N
IPR036422 RuBisCO_lsu_N_sf
IPR020888 RuBisCO_lsuI
PANTHERiPTHR42704 PTHR42704, 1 hit
PfamiView protein in Pfam
PF00016 RuBisCO_large, 1 hit
PF02788 RuBisCO_large_N, 1 hit
SFLDiSFLDS00014 RuBisCO, 1 hit
SUPFAMiSSF51649 SSF51649, 1 hit
SSF54966 SSF54966, 1 hit
PROSITEiView protein in PROSITE
PS00157 RUBISCO_LARGE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRBL_PARP8
AccessioniPrimary (citable) accession number: B2JX50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: June 10, 2008
Last modified: October 10, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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