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Protein

Biotin synthase

Gene

bioB

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi58Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi61Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi98Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi130Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi190Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi262Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciNPUN63737:G1GBR-5873-MONOMER
UniPathwayi
UPA00078;UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Npun_R5730
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000001191 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003815101 – 335Biotin synthaseAdd BLAST335

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi63737.Npun_R5730

Structurei

3D structure databases

ProteinModelPortaliB2J914
SMRiB2J914
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QSQ Bacteria
COG0502 LUCA
HOGENOMiHOG000239957
KOiK01012
OMAiADRFCMG
OrthoDBiPOG091H01DF
PhylomeDBiB2J914

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

B2J914-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVGIRYDWQE LEIRAIYNTP LLELIYQAAS VHRQYHDPTK IQVCKLISIK
60 70 80 90 100
TGGCPEDCSY CAQSSRYKTE VKAEALLEKE TVVNIAQKAK ETGVSRICMG
110 120 130 140 150
AAWREVRDNS QFEEVLEMVK DITAMGLEVC CTLGMLTANQ ARKLEEAGLY
160 170 180 190 200
AYNHNLDTSQ EYYSTIITTR TYSDRLNTIE NVRQTNVTVC SGGILGLGET
210 220 230 240 250
VDDRVGMLQT LANLHPHPES VPINILSQVP GTPLENQPDV PIWDIVRMIA
260 270 280 290 300
TARILMPASD VRLSAGRARL SQVEQAFCFM AGANSIFSSD DNKMLTVTTP
310 320 330
CPDYDTDREM LNLLGLGMRP PSQRQEKVAS PAVVG
Length:335
Mass (Da):37,213
Last modified:June 10, 2008 - v1
Checksum:i3C219BE1380461BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA Translation: ACC84030.1
RefSeqiWP_012411973.1, NC_010628.1

Genome annotation databases

EnsemblBacteriaiACC84030; ACC84030; Npun_R5730
KEGGinpu:Npun_R5730

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA Translation: ACC84030.1
RefSeqiWP_012411973.1, NC_010628.1

3D structure databases

ProteinModelPortaliB2J914
SMRiB2J914
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi63737.Npun_R5730

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC84030; ACC84030; Npun_R5730
KEGGinpu:Npun_R5730

Phylogenomic databases

eggNOGiENOG4107QSQ Bacteria
COG0502 LUCA
HOGENOMiHOG000239957
KOiK01012
OMAiADRFCMG
OrthoDBiPOG091H01DF
PhylomeDBiB2J914

Enzyme and pathway databases

UniPathwayi
UPA00078;UER00162

BioCyciNPUN63737:G1GBR-5873-MONOMER

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBIOB_NOSP7
AccessioniPrimary (citable) accession number: B2J914
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: October 10, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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