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UniProtKB - B2J4A4 (GERAS_NOSP7)
Protein
Germacrene A synthase
Gene
Npun_R3832
Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Functioni
Catalyzes the cyclization of farnesyl diphosphate (FPP) to the sesquiterpene germacrene A.
1 PublicationCatalytic activityi
- EC:4.2.3.901 Publication
Cofactori
Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity
Kineticsi
kcat is 0.044 sec(-1) with FPP as substrate (at pH 8).
- KM=3.4 µM for FPP (at pH 8)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 82 | Magnesium 1By similarity | 1 | |
Metal bindingi | 82 | Magnesium 2By similarity | 1 | |
Metal bindingi | 86 | Magnesium 1By similarity | 1 | |
Metal bindingi | 86 | Magnesium 2By similarity | 1 | |
Metal bindingi | 222 | Magnesium 3By similarity | 1 | |
Metal bindingi | 226 | Magnesium 3By similarity | 1 | |
Metal bindingi | 230 | Magnesium 3By similarity | 1 |
GO - Molecular functioni
- germacrene-A synthase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- sesquiterpene biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Lyase |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Germacrene A synthase (EC:4.2.3.90)Alternative name(s): 5-epi-alpha-selinene synthase 8a-epi-alpha-selinene synthase Terpene synthase |
Gene namesi | Ordered Locus Names:Npun_R3832 |
Organismi | Nostoc punctiforme (strain ATCC 29133 / PCC 73102) |
Taxonomic identifieri | 63737 [NCBI] |
Taxonomic lineagei | Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418841 | 1 – 323 | Germacrene A synthaseAdd BLAST | 323 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 82 – 86 | DDXXD motif | 5 |
Domaini
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similaritiesi
Belongs to the terpene synthase family.Curated
Phylogenomic databases
eggNOGi | COG0664, Bacteria |
HOGENOMi | CLU_042538_4_2_3 |
OMAi | DLIEYAM |
OrthoDBi | 1869158at2 |
PhylomeDBi | B2J4A4 |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034686, Terpene_cyclase-like_2 |
SFLDi | SFLDG01020, Terpene_Cyclase_Like_2, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
i Sequence
Sequence statusi: Complete.
B2J4A4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNQLLCPGLY CPFPSQTNKY VDVLEEYSLE WVLRFNLLAN ESAYKRFCKS
60 70 80 90 100
KFFFLAASAY PDSKFEELKI THDWLSWVFI WDDQCDLSEL KKQPEVLNNF
110 120 130 140 150
HQRYLEILNG AELTSQDTLF SHALIDLRKR TLQRASIKWF NYFISYLEDY
160 170 180 190 200
FYGCVQEATN RAKGIVPDLD TYIMIRRSSV GVYAVLALSE FCNQFIIPDV
210 220 230 240 250
LRNHHLVKKL ELITTDIIAW SNDIFSASRE IASGDVHNLI FVLHYHKKIS
260 270 280 290 300
LEKAIEQVVK IHNEEVHSLI KVESSLSFFS EELDVEITKY ISGMHSWIRG
310 320
NLDWCYESYR YHNLERLELT EFK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001037 Genomic DNA Translation: ACC82216.1 |
RefSeqi | WP_012410187.1, NC_010628.1 |
Genome annotation databases
EnsemblBacteriai | ACC82216; ACC82216; Npun_R3832 |
KEGGi | npu:Npun_R3832 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001037 Genomic DNA Translation: ACC82216.1 |
RefSeqi | WP_012410187.1, NC_010628.1 |
3D structure databases
SMRi | B2J4A4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 63737.Npun_R3832 |
Genome annotation databases
EnsemblBacteriai | ACC82216; ACC82216; Npun_R3832 |
KEGGi | npu:Npun_R3832 |
Phylogenomic databases
eggNOGi | COG0664, Bacteria |
HOGENOMi | CLU_042538_4_2_3 |
OMAi | DLIEYAM |
OrthoDBi | 1869158at2 |
PhylomeDBi | B2J4A4 |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034686, Terpene_cyclase-like_2 |
SFLDi | SFLDG01020, Terpene_Cyclase_Like_2, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GERAS_NOSP7 | |
Accessioni | B2J4A4Primary (citable) accession number: B2J4A4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | June 10, 2008 | |
Last modified: | February 23, 2022 | |
This is version 53 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families