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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurL (purL), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (purS)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei53UniRule annotation1
Binding sitei56ATPUniRule annotation1
Binding sitei95ATPUniRule annotation1
Metal bindingi97Magnesium 1UniRule annotation1
Active sitei99Proton acceptorUniRule annotation1
Binding sitei120SubstrateUniRule annotation1
Metal bindingi121Magnesium 2UniRule annotation1
Binding sitei244SubstrateUniRule annotation1
Metal bindingi274Magnesium 2UniRule annotation1
Binding sitei501ATPUniRule annotation1
Binding sitei538ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi539Magnesium 1UniRule annotation1
Binding sitei541SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLFER334390:G1G2I-139-MONOMER
UniPathwayi
UPA00074;UER00128

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Short name:
FGAR amidotransferase IIUniRule annotation
Short name:
FGAR-AT IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:LAF_0127
OrganismiLactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Taxonomic identifieri334390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000001697 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001948261 – 741Phosphoribosylformylglycinamidine synthase subunit PurLAdd BLAST741

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB2GF74
SMRiB2GF74
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 101Substrate bindingUniRule annotation4
Regioni318 – 320Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000238227
KOiK01952
OMAiFIEPYQG
OrthoDBiPOG091H009J

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.90.650.10, 3 hits
HAMAPiMF_00420 PurL_2, 1 hit
InterProiView protein in InterPro
IPR010074 PRibForGlyAmidine_synth_PurL
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
PANTHERiPTHR43555 PTHR43555, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PIRSFiPIRSF001587 FGAM_synthase_II, 1 hit
SUPFAMiSSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR01736 FGAM_synth_II, 1 hit

Sequencei

Sequence statusi: Complete.

B2GF74-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKQALTPEQI KEQKPYLDWS LSEREYDYIS EKLLGRLPNF TETGLFSAMW
60 70 80 90 100
SEHCSYKKSK PVLRLFPNQN DRVLQGPGEG AGVVDIDDGQ AVVFKAESHN
110 120 130 140 150
HPSTVEPYQG AATGVGGILR DIFSMGARPI ASLDSLHFGE LDDPKMQMKV
160 170 180 190 200
AGTVKGIGDY GNCMGIPTVA GETTFDPCYS GNLLVNAMSV GLMDQKDIQK
210 220 230 240 250
GKAAGIGNAV MYVGAKTGRD GIHGATFASA DFSDENATQR SAVQVGDPFM
260 270 280 290 300
EKLLLEACLE LIQNHPDWLV GIQDMGAAGI VSSSAEMASE GDAGMDLDLD
310 320 330 340 350
LVPQREPNMS AYEIMLSESQ ERMLLCVNKG HEDDVKKIFD FYGLEAVTIG
360 370 380 390 400
RITEGRQYVL HHDGQVVCDI PVSSLTDDVL EETSEEQKPA RILQAEQEAN
410 420 430 440 450
WQPEVTDAGA TLEQLLQQST IADKSIFFEQ YDSMVRTSTV VGPGSDAGVL
460 470 480 490 500
RVRGTKKGLA MTTDGNGRFV YLNPEVGGKM ALAEAAANIV ATGALPLAIT
510 520 530 540 550
DCLNYGDPND PEIFWELHQS VSGMAEACRV FDTPVISGNV SLYNENNGQA
560 570 580 590 600
IHPTPMVGMV GLIKDLKNLV KMAAQAAGDQ VYLLGQTGDD YAGSELQKML
610 620 630 640 650
TGDIAGTVAD FDLNHVHAMM KTLLSLMEDG KVASAHDLAE GGLGVALAET
660 670 680 690 700
LFKTDLGMNL KLDLTKNQLF SETAGRFVVT VKKEDAAAFE AALGDDATLI
710 720 730 740
GEVTNSHWLQ VRLADGNLNK NVADLEQLWK EAIPCQLKSR D
Length:741
Mass (Da):79,945
Last modified:June 10, 2008 - v1
Checksum:iEDD1CADA42CF4BC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008937 Genomic DNA Translation: BAG26463.1
RefSeqiWP_012390751.1, NC_010610.1

Genome annotation databases

EnsemblBacteriaiBAG26463; BAG26463; LAF_0127
GeneIDi6232958
KEGGilfe:LAF_0127
PATRICifig|334390.5.peg.133

Similar proteinsi

Entry informationi

Entry nameiPURL_LACF3
AccessioniPrimary (citable) accession number: B2GF74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: February 28, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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