Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

H(+)/Cl(-) exchange transporter ClcA

Gene

clcA

Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107ChlorideUniRule annotation1
Sitei148Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportUniRule annotation1
Sitei203Mediates proton transfer from the protein to the inner aqueous phaseUniRule annotation1
Binding sitei356Chloride; via amide nitrogenUniRule annotation1
Binding sitei357Chloride; via amide nitrogenUniRule annotation1
Binding sitei445ChlorideUniRule annotation1

GO - Molecular functioni

Keywordsi

Biological processAntiport, Ion transport, Transport
LigandChloride

Names & Taxonomyi

Protein namesi
Recommended name:
H(+)/Cl(-) exchange transporter ClcAUniRule annotation
Gene namesi
Name:clcAUniRule annotation
Synonyms:eriCUniRule annotation
Ordered Locus Names:ECDH10B_0135
OrganismiEscherichia coli (strain K12 / DH10B)
Taxonomic identifieri316385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32CytoplasmicUniRule annotationAdd BLAST32
Transmembranei33 – 69HelicalUniRule annotationAdd BLAST37
Topological domaini70 – 76PeriplasmicUniRule annotation7
Transmembranei77 – 100HelicalUniRule annotationAdd BLAST24
Intramembranei109 – 116HelicalUniRule annotation8
Topological domaini117 – 123CytoplasmicUniRule annotation7
Transmembranei124 – 141HelicalUniRule annotationAdd BLAST18
Transmembranei148 – 166HelicalUniRule annotationAdd BLAST19
Topological domaini167 – 176CytoplasmicUniRule annotation10
Intramembranei177 – 189HelicalUniRule annotationAdd BLAST13
Intramembranei193 – 201HelicalUniRule annotation9
Topological domaini202 – 214CytoplasmicUniRule annotationAdd BLAST13
Transmembranei215 – 232HelicalUniRule annotationAdd BLAST18
Topological domaini233 – 252PeriplasmicUniRule annotationAdd BLAST20
Transmembranei253 – 281HelicalUniRule annotationAdd BLAST29
Topological domaini282 – 287CytoplasmicUniRule annotation6
Transmembranei288 – 309HelicalUniRule annotationAdd BLAST22
Topological domaini310 – 329PeriplasmicUniRule annotationAdd BLAST20
Transmembranei330 – 349HelicalUniRule annotationAdd BLAST20
Transmembranei355 – 376HelicalUniRule annotationAdd BLAST22
Topological domaini377 – 386PeriplasmicUniRule annotation10
Intramembranei387 – 401HelicalUniRule annotationAdd BLAST15
Intramembranei402 – 404Note=Loop between two helicesUniRule annotation3
Intramembranei405 – 416HelicalUniRule annotationAdd BLAST12
Intramembranei417 – 421Note=Loop between two helicesUniRule annotation5
Transmembranei422 – 438HelicalUniRule annotationAdd BLAST17
Topological domaini439 – 473CytoplasmicUniRule annotationAdd BLAST35

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001372971 – 473H(+)/Cl(-) exchange transporter ClcAAdd BLAST473

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1XD25
SMRiB1XD25
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi106 – 110Selectivity filter part_1UniRule annotation5
Motifi146 – 150Selectivity filter part_2UniRule annotation5
Motifi355 – 359Selectivity filter part_3UniRule annotation5

Sequence similaritiesi

Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CMQ Bacteria
COG0038 LUCA
HOGENOMiHOG000257546
KOiK03281
OMAiEGPTAQF

Family and domain databases

Gene3Di1.10.3080.10, 1 hit
HAMAPiMF_01128 CLC_ClcA, 1 hit
InterProiView protein in InterPro
IPR023861 Cl-channel_ClcA
IPR014743 Cl-channel_core
IPR001807 Cl-channel_volt-gated
PfamiView protein in Pfam
PF00654 Voltage_CLC, 1 hit
PRINTSiPR00762 CLCHANNEL
SUPFAMiSSF81340 SSF81340, 1 hit

Sequencei

Sequence statusi: Complete.

B1XD25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTDTPSLET PQAARLRRRQ LIRQLLERDK TPLAILFMAA VVGTLVGLAA
60 70 80 90 100
VAFDKGVAWL QNQRMGALVH TADNYPLLLT VAFLCSAVLA MFGYFLVRKY
110 120 130 140 150
APEAGGSGIP EIEGALEDQR PVRWWRVLPV KFFGGLGTLG GGMVLGREGP
160 170 180 190 200
TVQIGGNIGR MVLDIFRLKG DEARHTLLAT GAAAGLAAAF NAPLAGILFI
210 220 230 240 250
IEEMRPQFRY TLISIKAVFI GVIMSTIMYR IFNHEVALID VGKLSDAPLN
260 270 280 290 300
TLWLYLILGI IFGIFGPIFN KWVLGMQDLL HRVHGGNITK WVLMGGAIGG
310 320 330 340 350
LCGLLGFVAP ATSGGGFNLI PIATAGNFSM GMLVFIFVAR VITTLLCFSS
360 370 380 390 400
GAPGGIFAPM LALGTVLGTA FGMVAVELFP QYHLEAGTFA IAGMGALLAA
410 420 430 440 450
SIRAPLTGII LVLEMTDNYQ LILPMIITGL GATLLAQFTG GKPLYSAILA
460 470
RTLAKQEAEQ LARSKAASAS ENT
Length:473
Mass (Da):50,349
Last modified:May 20, 2008 - v1
Checksum:i0B1BC39B417E9690
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000948 Genomic DNA Translation: ACB01334.1
RefSeqiWP_000845394.1, NC_010473.1

Genome annotation databases

EnsemblBacteriaiACB01334; ACB01334; ECDH10B_0135
KEGGiecd:ECDH10B_0135

Similar proteinsi

Entry informationi

Entry nameiCLCA_ECODH
AccessioniPrimary (citable) accession number: B1XD25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 20, 2008
Last modified: March 28, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health