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UniProtKB - B1XAN4 (EMTA_ECODH)
Protein
Endo-type membrane-bound lytic murein transglycosylase A
Gene
emtA
Organism
Escherichia coli (strain K12 / DH10B)
Status
Functioni
Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Preferentially cleaves at a distance of more than two disaccharide units from the ends of the glycan chain.
UniRule annotationCatalytic activityi
- Endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation EC:4.2.2.n2
GO - Molecular functioni
- carbon-oxygen lyase activity, acting on polysaccharides Source: UniProtKB-UniRule
- lytic endotransglycosylase activity Source: InterPro
GO - Biological processi
- cell wall macromolecule catabolic process Source: UniProtKB-UniRule
- cell wall organization Source: UniProtKB-KW
- peptidoglycan metabolic process Source: InterPro
Keywordsi
Molecular function | Lyase |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
BioCyci | ECOL316385:ECDH10B_RS06420-MONOMER |
Protein family/group databases
CAZyi | GH23, Glycoside Hydrolase Family 23 |
Names & Taxonomyi
Protein namesi | Recommended name: Endo-type membrane-bound lytic murein transglycosylase AUniRule annotation (EC:4.2.2.n2UniRule annotation)Alternative name(s): Peptidoglycan lytic endotransglycosylaseUniRule annotation |
Gene namesi | Name:emtAUniRule annotation Ordered Locus Names:ECDH10B_1246 |
Organismi | Escherichia coli (strain K12 / DH10B) |
Taxonomic identifieri | 316385 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Subcellular locationi
Other locations
- Cell outer membrane UniRule annotation; Lipid-anchor UniRule annotation
Other locations
- cell outer membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell outer membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | UniRule annotationAdd BLAST | 15 | |
ChainiPRO_1000144953 | 16 – 203 | Endo-type membrane-bound lytic murein transglycosylase AAdd BLAST | 188 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 16 | N-palmitoyl cysteineUniRule annotation | 1 | |
Lipidationi | 16 | S-diacylglycerol cysteineUniRule annotation | 1 |
Keywords - PTMi
Lipoprotein, PalmitateFamily & Domainsi
Sequence similaritiesi
Belongs to the transglycosylase Slt family.UniRule annotation
Keywords - Domaini
SignalPhylogenomic databases
HOGENOMi | CLU_103257_0_0_6 |
OMAi | EVYRYMG |
Family and domain databases
HAMAPi | MF_01381, EmtA, 1 hit |
InterProi | View protein in InterPro IPR023946, EmtA IPR023346, Lysozyme-like_dom_sf IPR000189, Transglyc_AS IPR008258, Transglycosylase_SLT_dom_1 |
Pfami | View protein in Pfam PF01464, SLT, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit PS00922, TRANSGLYCOSYLASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
B1XAN4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLRWFAFLI VLLAGCSSKH DYTNPPWNAK VPVQRAMQWM PISQKAGAAW
60 70 80 90 100
GVDPQLITAI IAIESGGNPN AVSKSNAIGL MQLKASTSGR DVYRRMGWSG
110 120 130 140 150
EPTTSELKNP ERNISMGAAY LNILETGPLA GIEDPKVLQY ALVVSYANGA
160 170 180 190 200
GALLRTFSSD RKKAISKIND LDADEFLEHV ARNHPAPQAP RYIYKLEQAL
DAM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000948 Genomic DNA Translation: ACB02363.1 |
RefSeqi | WP_001301104.1, NC_010473.1 |
Genome annotation databases
KEGGi | ecd:ECDH10B_1246 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000948 Genomic DNA Translation: ACB02363.1 |
RefSeqi | WP_001301104.1, NC_010473.1 |
3D structure databases
AlphaFoldDBi | B1XAN4 |
SMRi | B1XAN4 |
ModBasei | Search... |
Protein family/group databases
CAZyi | GH23, Glycoside Hydrolase Family 23 |
Genome annotation databases
KEGGi | ecd:ECDH10B_1246 |
Phylogenomic databases
HOGENOMi | CLU_103257_0_0_6 |
OMAi | EVYRYMG |
Enzyme and pathway databases
BioCyci | ECOL316385:ECDH10B_RS06420-MONOMER |
Family and domain databases
HAMAPi | MF_01381, EmtA, 1 hit |
InterProi | View protein in InterPro IPR023946, EmtA IPR023346, Lysozyme-like_dom_sf IPR000189, Transglyc_AS IPR008258, Transglycosylase_SLT_dom_1 |
Pfami | View protein in Pfam PF01464, SLT, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit PS00922, TRANSGLYCOSYLASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EMTA_ECODH | |
Accessioni | B1XAN4Primary (citable) accession number: B1XAN4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 14, 2009 |
Last sequence update: | May 20, 2008 | |
Last modified: | May 25, 2022 | |
This is version 70 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |