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Protein

Bifunctional protein FolD

Gene

folD

Organism
Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei232NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 168NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:EcolC_3093
OrganismiEscherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Taxonomic identifieri481805 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000879001 – 288Bifunctional protein FolDAdd BLAST288

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1IZ72
SMRiB1IZ72
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0 Bacteria
COG0190 LUCA
HOGENOMiHOG000218242
KOiK01491
OMAiAGKLCGD

Family and domain databases

HAMAPiMF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020867 THF_DH/CycHdrlase_CS
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PANTHERiPTHR10025 PTHR10025, 1 hit
PfamiView protein in Pfam
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00766 THF_DHG_CYH_1, 1 hit
PS00767 THF_DHG_CYH_2, 1 hit

Sequencei

Sequence statusi: Complete.

B1IZ72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKIIDGKT IAQQVRSEVA QKVQARIAAG LRAPGLAVVL VGSNPASQIY
60 70 80 90 100
VASKRKACEE VGFVSRSYDL PETTSEAELL ELIDTLNADN TIDGILVQLP
110 120 130 140 150
LPAGIDNVKV LERIHPDKDV DGFHPYNVGR LCQRAPRLRP CTPRGIVTLL
160 170 180 190 200
ERYNIDTFGL NAVVIGASNI VGRPMSMELL LAGCTTTVTH RFTKNLRHHV
210 220 230 240 250
ENADLLIVAV GKPGFIPGDW IKEGAIVIDV GINRLENGKV VGDVVFEDAA
260 270 280
KRASYITPVP GGVGPMTVAT LIENTLQACV EYHDPQDE
Length:288
Mass (Da):31,044
Last modified:April 29, 2008 - v1
Checksum:i028039D7C611085D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000946 Genomic DNA Translation: ACA78717.1
RefSeqiWP_000729160.1, NC_010468.1

Genome annotation databases

EnsemblBacteriaiACA78717; ACA78717; EcolC_3093
KEGGiecl:EcolC_3093

Similar proteinsi

Entry informationi

Entry nameiFOLD_ECOLC
AccessioniPrimary (citable) accession number: B1IZ72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 29, 2008
Last modified: October 25, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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