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UniProtKB - B1B3P3 (N8DT1_SOPFL)
Protein
Naringenin 8-dimethylallyltransferase 1, chloroplastic
Gene
N8DT-1
Organism
Sophora flavescens (Ku shen)
Status
Functioni
Involved in the biosynthesis of sophoraflavanone G (SFG). Can use flavanones (naringenin, liquiritigenin and hesperetin) as substrates, but not flavonols or isoflavones. Shows a strict specificity for dimethylallyl diphosphate.
1 PublicationCatalytic activityi
- EC:2.5.1.701 Publication
Cofactori
Kineticsi
- KM=106 µM for dimethylallyl diphosphate1 Publication
- KM=55 µM for naringenin1 Publication
Temperature dependencei
Optimum temperature is 75 degrees Celsius.1 Publication
GO - Molecular functioni
- prenyltransferase activity Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Enzyme and pathway databases
BRENDAi | 2.5.1.70, 8929 |
Names & Taxonomyi
Protein namesi | Recommended name: Naringenin 8-dimethylallyltransferase 1, chloroplastic (EC:2.5.1.70)Short name: SfN8DT-1 |
Gene namesi | Name:N8DT-1 |
Organismi | Sophora flavescens (Ku shen) |
Taxonomic identifieri | 49840 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Fabales › Fabaceae › Papilionoideae › 50 kb inversion clade › genistoids sensu lato › core genistoids › Sophoreae › Sophora |
Subcellular locationi
Chloroplast
- chloroplast membrane 1 Publication; Multi-pass membrane protein 1 Publication
Chloroplast
- chloroplast Source: UniProtKB
- chloroplast membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 124 – 144 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 150 – 170 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 195 – 217 | HelicalSequence analysisAdd BLAST | 23 | |
Transmembranei | 222 – 239 | HelicalSequence analysisAdd BLAST | 18 | |
Transmembranei | 251 – 271 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 288 – 308 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 331 – 351 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 355 – 375 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 386 – 406 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Chloroplast, Membrane, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 23 | ChloroplastSequence analysisAdd BLAST | 23 | |
ChainiPRO_0000418449 | 24 – 410 | Naringenin 8-dimethylallyltransferase 1, chloroplasticAdd BLAST | 387 |
Expressioni
Tissue specificityi
Secifically expressed in root bark. Not detected in aerial tissues.1 Publication
Inductioni
Up-regulated by elicitor, methyl jasmonate and salicylic acid.1 Publication
Family & Domainsi
Sequence similaritiesi
Belongs to the UbiA prenyltransferase family.Curated
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd13960, PT_UbiA_HPT1, 1 hit |
Gene3Di | 1.10.357.140, 1 hit |
InterProi | View protein in InterPro IPR044502, AtHST-like IPR000537, UbiA_prenyltransferase IPR044878, UbiA_sf |
Pfami | View protein in Pfam PF01040, UbiA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
B1B3P3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSMLLASFP GASSITTGGS CLRSKQYAKN YDASSYVTTS WYKKRKIQKE
60 70 80 90 100
HCAAIFSKHN LKQHYKVNEG GSTSNTSKEC EKKYVVNAIS EQSFEYEPQT
110 120 130 140 150
RDPESIWDSV NDALDIFYKF CRPYAMFTIV LGATFKSLVA VEKLSDLSLA
160 170 180 190 200
FFIGWLQVVV AVICIHIFGV GLNQLCDIEI DKINKPDLPL ASGKLSFRNV
210 220 230 240 250
VIITASSLIL GLGFAWIVDS WPLFWTVFIS CMVASAYNVD LPLLRWKKYP
260 270 280 290 300
VLTAINFIAD VAVTRSLGFF LHMQTCVFKR PTTFPRPLIF CTAIVSIYAI
310 320 330 340 350
VIALFKDIPD MEGDEKFGIQ SLSLRLGPKR VFWICVSLLE MTYGVTILVG
360 370 380 390 400
ATSPILWSKI ITVLGHAVLA SVLWYHAKSV DLTSNVVLHS FYMFIWKLHT
410
AEYFLIPLFR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB325579 mRNA Translation: BAG12671.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB325579 mRNA Translation: BAG12671.1 |
3D structure databases
AlphaFoldDBi | B1B3P3 |
SMRi | B1B3P3 |
ModBasei | Search... |
Enzyme and pathway databases
BRENDAi | 2.5.1.70, 8929 |
Family and domain databases
CDDi | cd13960, PT_UbiA_HPT1, 1 hit |
Gene3Di | 1.10.357.140, 1 hit |
InterProi | View protein in InterPro IPR044502, AtHST-like IPR000537, UbiA_prenyltransferase IPR044878, UbiA_sf |
Pfami | View protein in Pfam PF01040, UbiA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | N8DT1_SOPFL | |
Accessioni | B1B3P3Primary (citable) accession number: B1B3P3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | April 29, 2008 | |
Last modified: | May 25, 2022 | |
This is version 24 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |