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Protein

THO complex subunit 2

Gene

Thoc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for efficient export of polyadenylated RNA and spliced mRNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. Plays a role for proper neuronal development.By similarity

GO - Molecular functioni

  • mRNA binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-72187 mRNA 3'-end processing

Names & Taxonomyi

Protein namesi
Recommended name:
THO complex subunit 2
Short name:
Tho2
Gene namesi
Name:Thoc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2442413 Thoc2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003843991 – 1594THO complex subunit 2Add BLAST1594

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1222PhosphoserineCombined sources1
Modified residuei1385PhosphothreonineBy similarity1
Modified residuei1390PhosphoserineBy similarity1
Modified residuei1393PhosphoserineCombined sources1
Modified residuei1417PhosphoserineCombined sources1
Modified residuei1450PhosphoserineBy similarity1
Modified residuei1486PhosphoserineBy similarity1
Modified residuei1516PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiB1AZI6
MaxQBiB1AZI6
PaxDbiB1AZI6
PeptideAtlasiB1AZI6
PRIDEiB1AZI6

PTM databases

iPTMnetiB1AZI6
PhosphoSitePlusiB1AZI6

Expressioni

Tissue specificityi

Expressed in the hippocampus and the cortical neurons.1 Publication

Gene expression databases

BgeeiENSMUSG00000037475 Expressed in 273 organ(s), highest expression level in rostral migratory stream
ExpressionAtlasiB1AZI6 baseline and differential
GenevisibleiB1AZI6 MM

Interactioni

Subunit structurei

Component of the THO complex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7; together with at least ALYREF/THOC4, DDX39B, SARNP/CIP29 and CHTOP, THO forms the transcription/export (TREX) complex which seems to have a dynamic structure involving ATP-dependent remodeling. Interacts with THOC1, POLDIP3 and ZC3H11A (By similarity).By similarity

Protein-protein interaction databases

BioGridi237091, 1 interactor
IntActiB1AZI6, 1 interactor
STRINGi10090.ENSMUSP00000044677

Structurei

3D structure databases

ProteinModelPortaliB1AZI6
SMRiB1AZI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili896 – 965Sequence analysisAdd BLAST70
Coiled coili1464 – 1491Sequence analysisAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi923 – 928Nuclear localization signalSequence analysis6

Sequence similaritiesi

Belongs to the THOC2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1874 Eukaryota
ENOG410XQ2A LUCA
GeneTreeiENSGT00710000106792
HOGENOMiHOG000048227
InParanoidiB1AZI6
KOiK12879
OMAiPVCDMLK
OrthoDBiEOG091G00GM
PhylomeDBiB1AZI6
TreeFamiTF313127

Family and domain databases

InterProiView protein in InterPro
IPR040007 Tho2
IPR021418 THO_THOC2_C
IPR021726 THO_THOC2_N
IPR032302 THOC2_N
PANTHERiPTHR21597 PTHR21597, 1 hit
PfamiView protein in Pfam
PF11262 Tho2, 1 hit
PF11732 Thoc2, 1 hit
PF16134 THOC2_N, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All

B1AZI6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAAVVVPA EWIKNWEKSG RGEFLHLCRI LSENKSHDSS TYRDFQQALY
60 70 80 90 100
ELSYHVIKGN LKHEQASSVL NDISEFREDM PSILADVFCI LDIETNCLEE
110 120 130 140 150
KSKRDYFTQL VLACLYLVSD TVLKERLDPE TLESLGLIKQ SQQFNQKSVK
160 170 180 190 200
IKTKLFYKQQ KFNLLREENE GYAKLIAELG QDLSGNITSD LILENIKSLI
210 220 230 240 250
GCFNLDPNRV LDVILEVFEC RPEHDDFFIS LLESYMSMCE PQTLCHILGF
260 270 280 290 300
KFKFYQEPSG ETPSSLYRVA AVLLQFNLID LDDLYVHLLP ADNCIMDEYK
310 320 330 340 350
REIVEAKQIV RKLTMVVLSS EKLDERDKEK DKDDEKVEKP PDNQKLGLLE
360 370 380 390 400
ALLKVGDWQH AQNIMDQMPP YYAASHKLIA LAICKLIHIT VEPLYRRVGV
410 420 430 440 450
PKGAKGSPVS ALQNKRAPKQ VESFEDLRRD VFNMFCYLGP HLSHDPILFA
460 470 480 490 500
KVVRIGKSFM KEFQSDGSKQ EDKEKTEVIL SCLLSITDQV LLPSLSLMDC
510 520 530 540 550
NACMSEELWG MFKTFPYQHR YRLYGQWKNE TYNGHPLLVK VKAQTIDRAK
560 570 580 590 600
YIMKRLTKEN VKPSGRQIGK LSHSNPTILF DYILSQIQKY DNLITPVVDS
610 620 630 640 650
LKYLTSLNYD VLAYCIIEAL ANPEKERMKH DDTTISSWLQ SLASFCGAVF
660 670 680 690 700
RKYPIDLAGL LQYVANQLKA GKSFDLLILK EVVQKMAGIE ITEEMTMEQL
710 720 730 740 750
EAMTGGEQLK AEGGYFGQIR NTKKSSQRLK DALLDHDLAL PLCLLMAQQR
760 770 780 790 800
NGVIFQEGGE KHLKLVGKLY DQCHDTLVQF GGFLASNLST EDYIKRVPSI
810 820 830 840 850
DVLCNEFHTP HDAAFFLSRP MYAHHISSKY DELKKSEKGS KQQHKVHKYI
860 870 880 890 900
TSCEMVMAPV HEAVVSLHVS KVWDDISPQF YATFWSLTMY DLAVPHTSYE
910 920 930 940 950
REVNKLKVQM KAIDDNQEMP PNKKKKEKER CTALQDKLLE EEKKQMEHVQ
960 970 980 990 1000
RVLQRLKLEK DNWLLAKSTK NETITKFLQL CIFPRCIFSA IDAVYCARFV
1010 1020 1030 1040 1050
ELVHQQKTPN FSTLLCYDRV FSDIIYTVAS CTENEASRYG RFLCCMLETV
1060 1070 1080 1090 1100
TRWHSDRATY EKECGNYPGF LTILRATGFD GGNKADQLDY ENFRHVVHKW
1110 1120 1130 1140 1150
HYKLTKASVH CLETGEYTHI RNILIVLTKI LPWYPKVLNL GQALERRVNK
1160 1170 1180 1190 1200
ICQEEKEKRP DLYALAMGYS GQLKSRKSHM IPENEFHHKD PPPRNAVASV
1210 1220 1230 1240 1250
QNGPGGGTSS SSIGNASKSD ESGAEETDKS RERSQCGTKA VNKASSTTPK
1260 1270 1280 1290 1300
GNSSNGNSGS NSNKAVKEND KEKVKEKEKE KKEKTPATTP EARALGKDSK
1310 1320 1330 1340 1350
EKPKEERPNK EDKARETKER TPKSDKEKEK FKKEEKAKDE KFKTTVPIVE
1360 1370 1380 1390 1400
SKSTQERERE KEPSRERDVA KEMKSKENVK GGEKTPVSGS LKSPVPRSDI
1410 1420 1430 1440 1450
SEPDREQKRR KIDSHPSPSH SSTVKDSLID LKDSSAKLYI NHNPPPLSKS
1460 1470 1480 1490 1500
KEREMDKKDL DKSRERSRER EKKDEKDRKE RKRDHSNNDR EVPPDITKRR
1510 1520 1530 1540 1550
KEENGTMGVS KHKSESPCES QYPNEKDKEK NKSKSSGKEK SSSDSFKSEK
1560 1570 1580 1590
MDKISSGGKK ESRHDKEKIE KKEKRDSSGG KEEKKHHKSS DKHR
Length:1,594
Mass (Da):182,773
Last modified:April 8, 2008 - v1
Checksum:i9732F53F9C506CC8
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B8A4C2B8A4C2_MOUSE
THO complex subunit 2
Thoc2
109Annotation score:
F6RBN0F6RBN0_MOUSE
THO complex subunit 2
Thoc2
125Annotation score:
F6QCT0F6QCT0_MOUSE
THO complex subunit 2
Thoc2
53Annotation score:
B1AZI7B1AZI7_MOUSE
THO complex subunit 2
Thoc2
87Annotation score:
F6SBF2F6SBF2_MOUSE
THO complex subunit 2
Thoc2
165Annotation score:
F6VK25F6VK25_MOUSE
THO complex subunit 2
Thoc2
117Annotation score:
F6W2C7F6W2C7_MOUSE
THO complex subunit 2
Thoc2
86Annotation score:
Q8C690Q8C690_MOUSE
THO complex subunit 2
Thoc2
232Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX005253, AL954355 Genomic DNA Translation: CAM20174.1
AL954355, BX005253 Genomic DNA Translation: CAM27369.1
CCDSiCCDS40951.1
RefSeqiNP_001028594.1, NM_001033422.1
UniGeneiMm.259498

Genome annotation databases

EnsembliENSMUST00000047037; ENSMUSP00000044677; ENSMUSG00000037475
GeneIDi331401
KEGGimmu:331401
UCSCiuc009tao.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX005253, AL954355 Genomic DNA Translation: CAM20174.1
AL954355, BX005253 Genomic DNA Translation: CAM27369.1
CCDSiCCDS40951.1
RefSeqiNP_001028594.1, NM_001033422.1
UniGeneiMm.259498

3D structure databases

ProteinModelPortaliB1AZI6
SMRiB1AZI6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi237091, 1 interactor
IntActiB1AZI6, 1 interactor
STRINGi10090.ENSMUSP00000044677

PTM databases

iPTMnetiB1AZI6
PhosphoSitePlusiB1AZI6

Proteomic databases

EPDiB1AZI6
MaxQBiB1AZI6
PaxDbiB1AZI6
PeptideAtlasiB1AZI6
PRIDEiB1AZI6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047037; ENSMUSP00000044677; ENSMUSG00000037475
GeneIDi331401
KEGGimmu:331401
UCSCiuc009tao.1 mouse

Organism-specific databases

CTDi57187
MGIiMGI:2442413 Thoc2

Phylogenomic databases

eggNOGiKOG1874 Eukaryota
ENOG410XQ2A LUCA
GeneTreeiENSGT00710000106792
HOGENOMiHOG000048227
InParanoidiB1AZI6
KOiK12879
OMAiPVCDMLK
OrthoDBiEOG091G00GM
PhylomeDBiB1AZI6
TreeFamiTF313127

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-72187 mRNA 3'-end processing

Miscellaneous databases

ChiTaRSiThoc2 mouse
PROiPR:B1AZI6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037475 Expressed in 273 organ(s), highest expression level in rostral migratory stream
ExpressionAtlasiB1AZI6 baseline and differential
GenevisibleiB1AZI6 MM

Family and domain databases

InterProiView protein in InterPro
IPR040007 Tho2
IPR021418 THO_THOC2_C
IPR021726 THO_THOC2_N
IPR032302 THOC2_N
PANTHERiPTHR21597 PTHR21597, 1 hit
PfamiView protein in Pfam
PF11262 Tho2, 1 hit
PF11732 Thoc2, 1 hit
PF16134 THOC2_N, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTHOC2_MOUSE
AccessioniPrimary (citable) accession number: B1AZI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 8, 2008
Last modified: November 7, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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