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Protein

Transcriptional repressor NF-X1

Gene

Nfx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Together with PABPC1 or PABPC4, acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri352 – 403RING-type; atypicalPROSITE-ProRule annotationAdd BLAST52
Zinc fingeri447 – 465NF-X1-type 1Add BLAST19
Zinc fingeri500 – 519NF-X1-type 2Add BLAST20
Zinc fingeri561 – 580NF-X1-type 3Add BLAST20
Zinc fingeri626 – 649NF-X1-type 4Add BLAST24
Zinc fingeri688 – 707NF-X1-type 5Add BLAST20
Zinc fingeri715 – 734NF-X1-type 6Add BLAST20
Zinc fingeri826 – 848NF-X1-type 7Add BLAST23
Zinc fingeri857 – 878NF-X1-type 8Add BLAST22

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor, Transferase
Biological processTranscription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor NF-X1 (EC:2.3.2.-)
Short name:
m-Nfx.1
Alternative name(s):
Nuclear transcription factor, X box-binding protein 1
Gene namesi
Name:Nfx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1921414 Nfx1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003346141 – 1114Transcriptional repressor NF-X1Add BLAST1114

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineBy similarity1
Modified residuei81PhosphoserineCombined sources1
Modified residuei92PhosphoserineBy similarity1
Modified residuei126PhosphoserineCombined sources1
Modified residuei130PhosphoserineCombined sources1
Modified residuei147PhosphoserineBy similarity1
Modified residuei320PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiB1AY10
MaxQBiB1AY10
PaxDbiB1AY10
PeptideAtlasiB1AY10
PRIDEiB1AY10

PTM databases

iPTMnetiB1AY10
PhosphoSitePlusiB1AY10

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in thymus.1 Publication

Developmental stagei

Ubiquitously expressed at E12 and E14.1 Publication

Gene expression databases

BgeeiENSMUSG00000028423 Expressed in 275 organ(s), highest expression level in pituitary gland
GenevisibleiB1AY10 MM

Interactioni

Subunit structurei

Interacts with PABPC1 and PABPC4.By similarity

Protein-protein interaction databases

BioGridi216539, 1 interactor
IntActiB1AY10, 1 interactor
STRINGi10090.ENSMUSP00000095747

Structurei

3D structure databases

ProteinModelPortaliB1AY10
SMRiB1AY10
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini988 – 1056R3HPROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 26Interaction with PABPC1 and PABC4By similarityAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1078 – 1083Poly-Pro6

Domaini

The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.By similarity

Sequence similaritiesi

Belongs to the NFX1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri352 – 403RING-type; atypicalPROSITE-ProRule annotationAdd BLAST52
Zinc fingeri447 – 465NF-X1-type 1Add BLAST19
Zinc fingeri500 – 519NF-X1-type 2Add BLAST20
Zinc fingeri561 – 580NF-X1-type 3Add BLAST20
Zinc fingeri626 – 649NF-X1-type 4Add BLAST24
Zinc fingeri688 – 707NF-X1-type 5Add BLAST20
Zinc fingeri715 – 734NF-X1-type 6Add BLAST20
Zinc fingeri826 – 848NF-X1-type 7Add BLAST23
Zinc fingeri857 – 878NF-X1-type 8Add BLAST22

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1952 Eukaryota
ENOG410XR02 LUCA
GeneTreeiENSGT00400000022083
HOVERGENiHBG003827
InParanoidiB1AY10
KOiK12236
OMAiHPCEHPP
OrthoDBiEOG091G057V
PhylomeDBiB1AY10
TreeFamiTF105889

Family and domain databases

CDDicd02643 R3H_NF-X1, 1 hit
Gene3Di3.30.1370.50, 1 hit
InterProiView protein in InterPro
IPR034078 NFX1_fam
IPR001374 R3H_dom
IPR036867 R3H_dom_sf
IPR034076 R3H_NF-X1
IPR019786 Zinc_finger_PHD-type_CS
IPR000967 Znf_NFX1
IPR019787 Znf_PHD-finger
IPR001841 Znf_RING
PANTHERiPTHR12360 PTHR12360, 1 hit
PfamiView protein in Pfam
PF01424 R3H, 1 hit
PF01422 zf-NF-X1, 8 hits
SMARTiView protein in SMART
SM00393 R3H, 1 hit
SM00438 ZnF_NFX, 9 hits
SUPFAMiSSF82708 SSF82708, 1 hit
PROSITEiView protein in PROSITE
PS51061 R3H, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: B1AY10-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAEAPPVSGT FKFNTDAAEF IPQERKTSGL NCGTQRRLDS SRIGRRNYSS
60 70 80 90 100
SPPCHLPRHI PYEDISAVHQ HSYASGSKPK SPQGFFQSSN KSLKNHGLQN
110 120 130 140 150
QPWQKARNEK HQNRNKKAQG LSEQTSDTSS LESVARSESG TNPREHSPSE
160 170 180 190 200
SEKEVVIADP RGAKPKKAAQ LTYNYGRGPK AKGRLRSEWG NRMSPKSEDE
210 220 230 240 250
NTRPVAISHT DSSDASCRKP VVDPCVCRRN EQRRYPQKRP PWEVEGARPR
260 270 280 290 300
PGRNPPKQES QRHINAGPKT NMSPIPKDNL RERPTKSACD TGNLAVVSKS
310 320 330 340 350
SRRVNQEKTA VRRQDPQVLS PFPRGKQNHM LKNVETHTGS LIEQLTTEKY
360 370 380 390 400
ECMVCCELVQ VTAPVWSCQS CFHVFHLNCI KKWARSPASH ADGQSGWRCP
410 420 430 440 450
ACQNVSAHVP NTYTCFCGKV KNPEWSRNEI PHSCGEVCRK KQPGQDCPHS
460 470 480 490 500
CNLLCHPGPC PPCPAFTTKT CECGRTRHTV RCGQPVSVHC SNACENILNC
510 520 530 540 550
GQHHCAELCH GGQCQPCRII LNQVCYCGST SRDVLCGTDV GKSDGFGDFS
560 570 580 590 600
CLKICGKDLK CGSHTCSQVC HPQPCQPCPR LPHLVRYCPC GQTPLSQLLE
610 620 630 640 650
HGSNARKTCM DPVPSCGKVC GKPLACGSSD FIHTCEKLCH EGDCGPCSRT
660 670 680 690 700
SVISCRCSFR TKELPCTSLK SEDATFMCDK RCNKKRLCGR HKCNEICCVD
710 720 730 740 750
KEHKCPLICG RKLRCGLHRC EEPCHRGNCQ TCWQASFDEL TCHCGASVIY
760 770 780 790 800
PPVPCGTRPP ECTQTCARIH ECDHPVYHSC HSEEKCPPCT FLTQKWCMGK
810 820 830 840 850
HELRSNIPCH LVDISCGLPC SAMLPCGMHK CQRLCHKGEC LVDEACKQPC
860 870 880 890 900
TTPRGDCGHP CMAPCHPSLP CPVTACKAKV ELQCECGRRK EMVICSEASG
910 920 930 940 950
TYQRIVAISM ASKITDMQLG DSVEISKLIT KKEVQQARLQ CDEECAALER
960 970 980 990 1000
RKRLAEAFDI TDDSDPFNVR SSASKFSDSL KDDARKDLKF VSDVEKEMET
1010 1020 1030 1040 1050
LVEAVNKGKN NKKSHCFPPM NRDHRRIIHD LAQVYGLESI SYDSEPKRNV
1060 1070 1080 1090 1100
VVTAVRGKSV CPPTTLTSVI ERETQTRPPP PIPHHRHQAD KAPGSSTLQK
1110
IVKEAVIDYF DVQD
Length:1,114
Mass (Da):123,811
Last modified:April 8, 2008 - v1
Checksum:iA31013ACC6FB31F4
GO
Isoform 2 (identifier: B1AY10-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     803-804: LR → RQ
     805-1114: Missing.

Show »
Length:804
Mass (Da):89,439
Checksum:i0EC4111EF5F6C6E8
GO
Isoform 3 (identifier: B1AY10-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     803-818: LRSNIPCHLVDISCGL → ELTIKKLWTFKETLDF
     819-1114: Missing.

Show »
Length:818
Mass (Da):91,149
Checksum:iE803AE670CF16778
GO

Sequence cautioni

The sequence AAF34700 differs from that shown. Reason: Frameshift at positions 200, 214, 242 and 246.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49S → N in BAC28494 (PubMed:16141072).Curated1
Sequence conflicti141T → A in BAE33235 (PubMed:16141072).Curated1
Sequence conflicti273S → P in AAH53709 (PubMed:15489334).Curated1
Sequence conflicti455C → Y in BAE33235 (PubMed:16141072).Curated1
Sequence conflicti590C → R in AAH53709 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_033693803 – 818LRSNI…ISCGL → ELTIKKLWTFKETLDF in isoform 3. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_033694803 – 804LR → RQ in isoform 2. 2 Publications2
Alternative sequenceiVSP_033695805 – 1114Missing in isoform 2. 2 PublicationsAdd BLAST310
Alternative sequenceiVSP_033696819 – 1114Missing in isoform 3. 1 PublicationAdd BLAST296

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF223576 mRNA Translation: AAF34700.1 Frameshift.
AK005038 mRNA Translation: BAB23765.1
AK033850 mRNA Translation: BAC28494.1
AK144647 mRNA Translation: BAE25987.1
AK146108 mRNA Translation: BAE26907.1
AK155387 mRNA Translation: BAE33235.1
AL837521 Genomic DNA No translation available.
BC053709 mRNA Translation: AAH53709.1
CCDSiCCDS18054.1 [B1AY10-1]
CCDS71359.1 [B1AY10-2]
CCDS71360.1 [B1AY10-3]
RefSeqiNP_001277377.1, NM_001290448.1 [B1AY10-3]
NP_001277378.1, NM_001290449.1 [B1AY10-2]
NP_076228.2, NM_023739.3 [B1AY10-1]
UniGeneiMm.247456
Mm.439181

Genome annotation databases

EnsembliENSMUST00000030133; ENSMUSP00000030133; ENSMUSG00000028423 [B1AY10-3]
ENSMUST00000091614; ENSMUSP00000089203; ENSMUSG00000028423 [B1AY10-2]
ENSMUST00000098143; ENSMUSP00000095747; ENSMUSG00000028423 [B1AY10-1]
GeneIDi74164
KEGGimmu:74164
UCSCiuc008sic.2 mouse [B1AY10-2]
uc008sid.2 mouse [B1AY10-3]
uc008sie.2 mouse [B1AY10-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF223576 mRNA Translation: AAF34700.1 Frameshift.
AK005038 mRNA Translation: BAB23765.1
AK033850 mRNA Translation: BAC28494.1
AK144647 mRNA Translation: BAE25987.1
AK146108 mRNA Translation: BAE26907.1
AK155387 mRNA Translation: BAE33235.1
AL837521 Genomic DNA No translation available.
BC053709 mRNA Translation: AAH53709.1
CCDSiCCDS18054.1 [B1AY10-1]
CCDS71359.1 [B1AY10-2]
CCDS71360.1 [B1AY10-3]
RefSeqiNP_001277377.1, NM_001290448.1 [B1AY10-3]
NP_001277378.1, NM_001290449.1 [B1AY10-2]
NP_076228.2, NM_023739.3 [B1AY10-1]
UniGeneiMm.247456
Mm.439181

3D structure databases

ProteinModelPortaliB1AY10
SMRiB1AY10
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216539, 1 interactor
IntActiB1AY10, 1 interactor
STRINGi10090.ENSMUSP00000095747

PTM databases

iPTMnetiB1AY10
PhosphoSitePlusiB1AY10

Proteomic databases

EPDiB1AY10
MaxQBiB1AY10
PaxDbiB1AY10
PeptideAtlasiB1AY10
PRIDEiB1AY10

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030133; ENSMUSP00000030133; ENSMUSG00000028423 [B1AY10-3]
ENSMUST00000091614; ENSMUSP00000089203; ENSMUSG00000028423 [B1AY10-2]
ENSMUST00000098143; ENSMUSP00000095747; ENSMUSG00000028423 [B1AY10-1]
GeneIDi74164
KEGGimmu:74164
UCSCiuc008sic.2 mouse [B1AY10-2]
uc008sid.2 mouse [B1AY10-3]
uc008sie.2 mouse [B1AY10-1]

Organism-specific databases

CTDi4799
MGIiMGI:1921414 Nfx1

Phylogenomic databases

eggNOGiKOG1952 Eukaryota
ENOG410XR02 LUCA
GeneTreeiENSGT00400000022083
HOVERGENiHBG003827
InParanoidiB1AY10
KOiK12236
OMAiHPCEHPP
OrthoDBiEOG091G057V
PhylomeDBiB1AY10
TreeFamiTF105889

Miscellaneous databases

ChiTaRSiNfx1 mouse
PROiPR:B1AY10
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028423 Expressed in 275 organ(s), highest expression level in pituitary gland
GenevisibleiB1AY10 MM

Family and domain databases

CDDicd02643 R3H_NF-X1, 1 hit
Gene3Di3.30.1370.50, 1 hit
InterProiView protein in InterPro
IPR034078 NFX1_fam
IPR001374 R3H_dom
IPR036867 R3H_dom_sf
IPR034076 R3H_NF-X1
IPR019786 Zinc_finger_PHD-type_CS
IPR000967 Znf_NFX1
IPR019787 Znf_PHD-finger
IPR001841 Znf_RING
PANTHERiPTHR12360 PTHR12360, 1 hit
PfamiView protein in Pfam
PF01424 R3H, 1 hit
PF01422 zf-NF-X1, 8 hits
SMARTiView protein in SMART
SM00393 R3H, 1 hit
SM00438 ZnF_NFX, 9 hits
SUPFAMiSSF82708 SSF82708, 1 hit
PROSITEiView protein in PROSITE
PS51061 R3H, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNFX1_MOUSE
AccessioniPrimary (citable) accession number: B1AY10
Secondary accession number(s): Q3U2A7
, Q3UK95, Q3UMW1, Q7TPT4, Q8CC59, Q9DBC8, Q9JKW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: November 7, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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