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Protein

Schlafen family member 8

Gene

Slfn8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Endoribonuclease that cleaves tRNAs and rRNAs (PubMed:29563550). Cleaves tRNAs 11 nucleotides from the 3'-terminus at the acceptor stem (PubMed:29563550). May be involved in immune system via regulation of inflammation (PubMed:29528433).2 Publications

Cofactori

Mg2+By similarityNote: Can also use Mn2+.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei205By similarity1
Active sitei210By similarity1
Metal bindingi280ZincBy similarity1
Metal bindingi282ZincBy similarity1
Metal bindingi319ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi599 – 606ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • endoribonuclease activity Source: UniProtKB
  • tRNA binding Source: GO_Central
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processImmunity
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Schlafen family member 8Curated (EC:3.1.-.-1 Publication)
Alternative name(s):
Schlafen-81 Publication
Short name:
mSLFN81 Publication
Gene namesi
Name:Slfn81 PublicationImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2672859 Slfn8

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype in normal conditions. Mice are resistant in experimental autoimmune encephalomyelitis (EAE) model, a T-cell-mediated autoimmune model. The expression of pro-inflammatory mediators is severely reduced in EAE. No dysfunction of T-cells, or other leukocytes is detected.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004446071 – 910Schlafen family member 8Add BLAST910

Proteomic databases

PaxDbiB1ARD8
PeptideAtlasiB1ARD8
PRIDEiB1ARD8

PTM databases

iPTMnetiB1ARD8
PhosphoSitePlusiB1ARD8

Expressioni

Tissue specificityi

In T-cells, expressed at relatively constant levels during development: expressed in immature CD3-CD4-CD8- T-cells (DN stage), in CD4+CD8+ double-positive stage (DP) and mature CD4+ or CD8+ thymocytes. Expression is slightly reduced at the DP stage.1 Publication

Inductioni

Induced following infection.1 Publication

Gene expression databases

BgeeiENSMUSG00000035208 Expressed in 132 organ(s), highest expression level in blood
ExpressionAtlasiB1ARD8 baseline and differential
GenevisibleiQ7TMF1 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040060

Structurei

3D structure databases

ProteinModelPortaliB1ARD8
SMRiB1ARD8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 354N'-domain regionBy similarityAdd BLAST354

Domaini

Shows a pseudo-dimeric U-pillow-shaped architecture of the SLFN13 N'-domain that may clamp base-paired RNAs.By similarity

Sequence similaritiesi

Belongs to the Schlafen family. Subgroup III subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IJEW Eukaryota
ENOG410Z5MV LUCA
GeneTreeiENSGT00410000025651
HOGENOMiHOG000049135
HOVERGENiHBG056880
InParanoidiB1ARD8
OMAiIANECHR
OrthoDBiEOG091G01HZ
PhylomeDBiB1ARD8
TreeFamiTF337168

Family and domain databases

Gene3Di3.30.950.30, 1 hit
InterProiView protein in InterPro
IPR018647 DUF2075
IPR027417 P-loop_NTPase
IPR029684 Schlafen
IPR007421 Schlafen_AAA_dom
IPR038461 Schlafen_AAA_dom_sf
IPR029679 SLFN8/9/13
PANTHERiPTHR12155 PTHR12155, 1 hit
PTHR12155:SF21 PTHR12155:SF21, 1 hit
PfamiView protein in Pfam
PF04326 AlbA_2, 1 hit
PF09848 DUF2075, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

B1ARD8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
METHPSLAVK WSCPDLTIYA GEVTIGEEDR NKMDSKKRKL EKTRITEAAC
60 70 80 90 100
ALLNSGGGLI AMQMTNKSEH PVEMGQDLEK SLRELIMSPN MQAFFETKQQ
110 120 130 140 150
EDQFYIFVKS WSCRPEDGST KPRICSLGSS LYCRSITSKV AMDSREAFEF
160 170 180 190 200
LKDKKACIKY RPTDDGAPPA KIPRAMCQNS LESNPAFEIF QSKKLEYGQC
210 220 230 240 250
LLFSESTSIE FKQFSTKHVQ AYMKNIIPEY ISAFANTQGG YLFIGVDDKR
260 270 280 290 300
IILGCPKDNV DRDSLKTVAN ETISKVPVFH FCSSKDKDKV SYETRVIDVF
310 320 330 340 350
QEGNLYGYLC VIKVEPFCCA VFSEAPISWM VDKEKGVYRL NTEEWVRMMV
360 370 380 390 400
DFGPEASSKD LSKDFECQLS LCNSPPHCRP VYSKKGLQHK VDLQQRLFQV
410 420 430 440 450
SPDCLKYTPE SLWKELCSQH KRLKGLVKQQ IRSFSCGLLI LYRSWAVDLN
460 470 480 490 500
LKEKQEVICD ALLIAQNSPP ILYTILGEQD EQGQDYCNHT AFTLKQKLVN
510 520 530 540 550
TGGYTGRVCV MTKVLCLSSQ NNIETNGGSV SPINYPSSYN LANIQEMQDL
560 570 580 590 600
LQALVIVLLN FRSFLSDQLG CEILNLLTAQ QYEILSKSLR KTRELFVHGL
610 620 630 640 650
PGSGKTIIAM KIMEKIRNTF HCETDSILYI CENQPLRDFI RAKRICQAVT
660 670 680 690 700
RKTFMNYRFK TNSFQHIIVD EAQNFRTEDG NWYGKAKAIS RRVKSCPGMF
710 720 730 740 750
WIFLDYFQTS HLKESGLPDF SRQYPREELT QVVRNGDKIA EFLQKELQKI
760 770 780 790 800
RDNPPCSIPR QSLNIVHEFK WSQSVSGNIK TEQFTLEDMV IYVADKCYDF
810 820 830 840 850
LRKGYSLQDI AVLFSTDKEK KTYESMFLGE MRKRRRASEM NHAYLCDSNM
860 870 880 890 900
FDSIRRFSGL ERSIVFGINP IATEQPISHN LLLCLASRAM KHLYILYFST
910
PEGHSSTEAC
Length:910
Mass (Da):104,264
Last modified:April 8, 2008 - v1
Checksum:iCEB49E7AA3F48CAE
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q0R4E9Q0R4_MOUSE
Schlafen family member 8
Slfn8
407Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti191Q → R in BAE29925 (PubMed:16141072).Curated1
Sequence conflicti899S → P in AAP30068 (PubMed:15351786).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY261798 mRNA Translation: AAP30068.1
AL603745 Genomic DNA No translation available.
AK150875 mRNA Translation: BAE29925.1
CCDSiCCDS25155.1
RefSeqiNP_853523.2, NM_181545.4
XP_006533547.1, XM_006533484.3
UniGeneiMm.491599

Genome annotation databases

EnsembliENSMUST00000038141; ENSMUSP00000040060; ENSMUSG00000035208
ENSMUST00000092838; ENSMUSP00000090513; ENSMUSG00000035208
ENSMUST00000229469; ENSMUSP00000155522; ENSMUSG00000115953
ENSMUST00000230972; ENSMUSP00000155276; ENSMUSG00000115953
GeneIDi276950
UCSCiuc007kod.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY261798 mRNA Translation: AAP30068.1
AL603745 Genomic DNA No translation available.
AK150875 mRNA Translation: BAE29925.1
CCDSiCCDS25155.1
RefSeqiNP_853523.2, NM_181545.4
XP_006533547.1, XM_006533484.3
UniGeneiMm.491599

3D structure databases

ProteinModelPortaliB1ARD8
SMRiB1ARD8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040060

PTM databases

iPTMnetiB1ARD8
PhosphoSitePlusiB1ARD8

Proteomic databases

PaxDbiB1ARD8
PeptideAtlasiB1ARD8
PRIDEiB1ARD8

Protocols and materials databases

DNASUi276950
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038141; ENSMUSP00000040060; ENSMUSG00000035208
ENSMUST00000092838; ENSMUSP00000090513; ENSMUSG00000035208
ENSMUST00000229469; ENSMUSP00000155522; ENSMUSG00000115953
ENSMUST00000230972; ENSMUSP00000155276; ENSMUSG00000115953
GeneIDi276950
UCSCiuc007kod.2 mouse

Organism-specific databases

CTDi276950
MGIiMGI:2672859 Slfn8

Phylogenomic databases

eggNOGiENOG410IJEW Eukaryota
ENOG410Z5MV LUCA
GeneTreeiENSGT00410000025651
HOGENOMiHOG000049135
HOVERGENiHBG056880
InParanoidiB1ARD8
OMAiIANECHR
OrthoDBiEOG091G01HZ
PhylomeDBiB1ARD8
TreeFamiTF337168

Miscellaneous databases

ChiTaRSiSlfn8 mouse
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035208 Expressed in 132 organ(s), highest expression level in blood
ExpressionAtlasiB1ARD8 baseline and differential
GenevisibleiQ7TMF1 MM

Family and domain databases

Gene3Di3.30.950.30, 1 hit
InterProiView protein in InterPro
IPR018647 DUF2075
IPR027417 P-loop_NTPase
IPR029684 Schlafen
IPR007421 Schlafen_AAA_dom
IPR038461 Schlafen_AAA_dom_sf
IPR029679 SLFN8/9/13
PANTHERiPTHR12155 PTHR12155, 1 hit
PTHR12155:SF21 PTHR12155:SF21, 1 hit
PfamiView protein in Pfam
PF04326 AlbA_2, 1 hit
PF09848 DUF2075, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSLFN8_MOUSE
AccessioniPrimary (citable) accession number: B1ARD8
Secondary accession number(s): A0A0A0MQG0
, F6RTS1, Q3UBN9, Q7TMF1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2018
Last sequence update: April 8, 2008
Last modified: November 7, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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