Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 36

Gene

Usp36

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Deubiquitinase essential for the regulation of nucleolar structure and function. Required for cell and organism viability. Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability (PubMed:29273634). Function as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm. Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions. Deubiquitinates SOD2, regulates SOD2 protein stability. Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins (By similarity).By similarity1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei131NucleophilePROSITE-ProRule annotation1
Active sitei382Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • histone deubiquitination Source: UniProtKB
  • negative regulation of macroautophagy Source: UniProtKB
  • nucleolus organization Source: UniProtKB
  • protein deubiquitination Source: UniProtKB
  • protein stabilization Source: UniProtKB
  • regulation of protein stability Source: UniProtKB
  • regulation of rRNA processing Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 36 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 36
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36
Gene namesi
Name:Usp36
Synonyms:Kiaa1453
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1919594 Usp36

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality (PubMed:29273634). Embryos show apoptosis at the morula stage, blocking the transition from morula to blastocysts during embryonic development (PubMed:29273634).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003784941 – 1098Ubiquitin carboxyl-terminal hydrolase 36Add BLAST1098

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei429PhosphoserineBy similarity1
Modified residuei463PhosphoserineBy similarity1
Modified residuei547PhosphoserineBy similarity1
Modified residuei578PhosphoserineBy similarity1
Modified residuei663PhosphoserineBy similarity1
Modified residuei678PhosphoserineBy similarity1
Modified residuei709PhosphoserineBy similarity1

Post-translational modificationi

Polyubiquitinated by NEDD4L, no effect on USP36 protein levels. Both proteins interact with and regulate each other's ubiquitination levels.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiB1AQJ2
MaxQBiB1AQJ2
PaxDbiB1AQJ2
PeptideAtlasiB1AQJ2
PRIDEiB1AQJ2

PTM databases

iPTMnetiB1AQJ2
PhosphoSitePlusiB1AQJ2

Expressioni

Gene expression databases

BgeeiENSMUSG00000033909
ExpressionAtlasiB1AQJ2 baseline and differential
GenevisibleiB1AQJ2 MM

Interactioni

Subunit structurei

Interacts with isoform 3 of FBXW7; the interaction inhibits MYC degradation induced by SCF(FBW7) complex. Interacts with NTRK1; USP36 does not deubiquitinate NTRK1. Interacts with NEDD4L (via domains WW1, 3 and 4); the interaction inhibits ubiquitination of, at least, NTRK1, KCNQ2 and KCNQ3 by NEDD4L.By similarity

Protein-protein interaction databases

BioGridi215324, 1 interactor
STRINGi10090.ENSMUSP00000090036

Structurei

3D structure databases

ProteinModelPortaliB1AQJ2
SMRiB1AQJ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini122 – 423USPAdd BLAST302

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

eggNOGiKOG1865 Eukaryota
ENOG410XQ92 LUCA
GeneTreeiENSGT00890000139343
HOGENOMiHOG000154759
InParanoidiB1AQJ2
KOiK11855
OMAiKRNFNAF
OrthoDBiEOG091G02D8
PhylomeDBiB1AQJ2
TreeFamiTF315281

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00443 UCH, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

B1AQJ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIVDKLKEA LKPGRKDSAE DGDLGRLLAA SAKKVLLQRI EFEPASKSFS
60 70 80 90 100
YQLESLKSKY VLLSARAEGA SRHRSGDELQ ARKPGTERVS GSGGDGVPAP
110 120 130 140 150
QKVLFPVERL SLRWERVFRV GAGLHNLGNT CFLNSTIQCL TYTPPLANYL
160 170 180 190 200
LSKEHARSCH QGGFCMLCLM QNHMVQAFAN SGNAIKPVSF IRDLKKIARH
210 220 230 240 250
FRFGNQEDAH EFLRYTIDAM QKACLNGYAK LDRQTQATTL VHQIFGGYLR
260 270 280 290 300
SRVKCSVCKS VSDTYDPYLD IALEIRQAAN IVRALELFVK SDVLSGENAY
310 320 330 340 350
MCAKCKKKVP ASKRFTIHRT SNVLTLSLKR FANFSGGKIT KDVGYPEFLN
360 370 380 390 400
IRPYMSQSSG DPVMYGLYAV LVHSGYSCHA GHYYCYVKAS NGQWYQMNDS
410 420 430 440 450
LVHSSNVKVV LNQQAYVLFY LRIPGSKKSP EGPVSRVGAT LPSRPKVVPE
460 470 480 490 500
HSKKSPGNGV VPSPLMAKRQ DSVMMRKLPA PEEVGVPVSR NGSLPGLKLQ
510 520 530 540 550
NGCAPAKTPA GSPSPRLTPT PTHMPTILDE PGKKVKKSAP LQSLTTSPTT
560 570 580 590 600
SQGSPGTGES RSQRPGSWAS RDTIFSTSPK LLARAITNGH RLKGEGSGVD
610 620 630 640 650
LEKGDSSSSS PEHSASSDPA KAPQTAESRA AHACDSQGTN CPTAGHPKAL
660 670 680 690 700
LNGVDAKMVK LKSPALSSTT TEPTSLMSPP PAKKLALSAK KASTLRRATG
710 720 730 740 750
NDIGSPSPSA FCDLTSPMKA THPVVASTGP VSKTRTAAPA PRPSTHPHSA
760 770 780 790 800
SLSSSSAKPL GTSEPQSCRP SAWTPLPQVN GHFTSHLHQL PEASEALHSP
810 820 830 840 850
SKKRKKTPNG DPQRLGIDTL LPQCLRGAPA AARRKRKKRC SEGEGATAPK
860 870 880 890 900
QEGQFQDQSW SSGSQKEEGT QPQVNGHQVS HILDSYHVSS RKRRKRKRSE
910 920 930 940 950
GLSQEATPSQ DLIQHSCSPV DHSEPEARTE LQKKKKKKRR KRKPEPQQDE
960 970 980 990 1000
ESKHPGDQRS PRPSVTPVPA LSVNGHLPSD CLGLGQAPLV TWNRDQEPDV
1010 1020 1030 1040 1050
VQALLQDSSD KAYGKKVLTW DGEPSAISQD AIKDSRLART QTVVDDWDEE
1060 1070 1080 1090
FDRGKEKKIK KFKREKKRNF NAFQKLQSRR NFWSVTHPAK VASLSYRR
Length:1,098
Mass (Da):119,913
Last modified:April 8, 2008 - v1
Checksum:i5853578D7BFD84DF
GO

Sequence cautioni

The sequence BAC98173 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti452S → P in BAC98173 (PubMed:14621295).Curated1
Sequence conflicti486V → I in BAC98173 (PubMed:14621295).Curated1
Sequence conflicti541L → P in BAC98173 (PubMed:14621295).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591404 Genomic DNA No translation available.
AK129363 mRNA Translation: BAC98173.1 Different initiation.
CCDSiCCDS48998.1
RefSeqiNP_001028700.1, NM_001033528.1
XP_006534365.1, XM_006534302.3
XP_006534366.1, XM_006534303.2
XP_006534367.1, XM_006534304.3
UniGeneiMm.232293

Genome annotation databases

EnsembliENSMUST00000092382; ENSMUSP00000090036; ENSMUSG00000033909
ENSMUST00000106296; ENSMUSP00000101903; ENSMUSG00000033909
GeneIDi72344
KEGGimmu:72344
UCSCiuc007mot.2 mouse

Similar proteinsi

Entry informationi

Entry nameiUBP36_MOUSE
AccessioniPrimary (citable) accession number: B1AQJ2
Secondary accession number(s): Q6ZPQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 8, 2008
Last modified: June 20, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health