UniProtKB - B1AQJ2 (UBP36_MOUSE)
Ubiquitin carboxyl-terminal hydrolase 36
Usp36
Functioni
Deubiquitinase essential for the regulation of nucleolar structure and function. Required for cell and organism viability. Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability (PubMed:29273634).
Functions as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm. Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions. Deubiquitinates SOD2, regulates SOD2 protein stability. Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins (By similarity).
By similarity1 PublicationCatalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 131 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 382 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: GO_Central
- thiol-dependent deubiquitinase Source: MGI
GO - Biological processi
- histone deubiquitination Source: UniProtKB
- negative regulation of macroautophagy Source: UniProtKB
- nucleolus organization Source: UniProtKB
- protein deubiquitination Source: UniProtKB
- protein stabilization Source: UniProtKB
- regulation of apoptotic process Source: GO_Central
- regulation of protein stability Source: UniProtKB
- regulation of rRNA processing Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Ubl conjugation pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin carboxyl-terminal hydrolase 36 (EC:3.4.19.12)Alternative name(s): Deubiquitinating enzyme 36 Ubiquitin thioesterase 36 Ubiquitin-specific-processing protease 36 |
Gene namesi | Name:Usp36 Synonyms:Kiaa1453 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919594, Usp36 |
VEuPathDBi | HostDB:ENSMUSG00000033909 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Nucleus
- nuclear speck Source: MGI
- nucleolus Source: UniProtKB
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000378494 | 1 – 1098 | Ubiquitin carboxyl-terminal hydrolase 36Add BLAST | 1098 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 429 | PhosphoserineBy similarity | 1 | |
Modified residuei | 463 | PhosphoserineBy similarity | 1 | |
Modified residuei | 547 | PhosphoserineBy similarity | 1 | |
Modified residuei | 578 | PhosphoserineBy similarity | 1 | |
Modified residuei | 663 | PhosphoserineBy similarity | 1 | |
Modified residuei | 678 | PhosphoserineBy similarity | 1 | |
Modified residuei | 709 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | B1AQJ2 |
MaxQBi | B1AQJ2 |
PaxDbi | B1AQJ2 |
PeptideAtlasi | B1AQJ2 |
PRIDEi | B1AQJ2 |
ProteomicsDBi | 298360 |
PTM databases
iPTMneti | B1AQJ2 |
PhosphoSitePlusi | B1AQJ2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000033909, Expressed in spleen and 285 other tissues |
ExpressionAtlasi | B1AQJ2, baseline and differential |
Genevisiblei | B1AQJ2, MM |
Interactioni
Subunit structurei
Interacts with isoform 3 of FBXW7; the interaction inhibits MYC degradation induced by SCF(FBW7) complex.
Interacts with NTRK1; USP36 does not deubiquitinate NTRK1.
Interacts with NEDD4L (via domains WW1, 3 and 4); the interaction inhibits ubiquitination of, at least, NTRK1, KCNQ2 and KCNQ3 by NEDD4L.
By similarityProtein-protein interaction databases
BioGRIDi | 215324, 1 interactor |
STRINGi | 10090.ENSMUSP00000101903 |
Miscellaneous databases
RNActi | B1AQJ2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 122 – 423 | USPAdd BLAST | 302 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 72 – 97 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 428 – 464 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 483 – 574 | DisorderedSequence analysisAdd BLAST | 92 | |
Regioni | 589 – 640 | DisorderedSequence analysisAdd BLAST | 52 | |
Regioni | 664 – 710 | DisorderedSequence analysisAdd BLAST | 47 | |
Regioni | 722 – 973 | DisorderedSequence analysisAdd BLAST | 252 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 539 – 574 | Polar residuesSequence analysisAdd BLAST | 36 | |
Compositional biasi | 602 – 623 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 694 – 710 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 746 – 787 | Polar residuesSequence analysisAdd BLAST | 42 | |
Compositional biasi | 797 – 811 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 851 – 885 | Polar residuesSequence analysisAdd BLAST | 35 | |
Compositional biasi | 903 – 917 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 943 – 958 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1865, Eukaryota |
GeneTreei | ENSGT00940000157948 |
HOGENOMi | CLU_008279_10_1_1 |
InParanoidi | B1AQJ2 |
OMAi | SETCLPQ |
OrthoDBi | 929408at2759 |
PhylomeDBi | B1AQJ2 |
TreeFami | TF315281 |
Family and domain databases
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF00443, UCH, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MPIVDKLKEA LKPGRKDSAE DGDLGRLLAA SAKKVLLQRI EFEPASKSFS
60 70 80 90 100
YQLESLKSKY VLLSARAEGA SRHRSGDELQ ARKPGTERVS GSGGDGVPAP
110 120 130 140 150
QKVLFPVERL SLRWERVFRV GAGLHNLGNT CFLNSTIQCL TYTPPLANYL
160 170 180 190 200
LSKEHARSCH QGGFCMLCLM QNHMVQAFAN SGNAIKPVSF IRDLKKIARH
210 220 230 240 250
FRFGNQEDAH EFLRYTIDAM QKACLNGYAK LDRQTQATTL VHQIFGGYLR
260 270 280 290 300
SRVKCSVCKS VSDTYDPYLD IALEIRQAAN IVRALELFVK SDVLSGENAY
310 320 330 340 350
MCAKCKKKVP ASKRFTIHRT SNVLTLSLKR FANFSGGKIT KDVGYPEFLN
360 370 380 390 400
IRPYMSQSSG DPVMYGLYAV LVHSGYSCHA GHYYCYVKAS NGQWYQMNDS
410 420 430 440 450
LVHSSNVKVV LNQQAYVLFY LRIPGSKKSP EGPVSRVGAT LPSRPKVVPE
460 470 480 490 500
HSKKSPGNGV VPSPLMAKRQ DSVMMRKLPA PEEVGVPVSR NGSLPGLKLQ
510 520 530 540 550
NGCAPAKTPA GSPSPRLTPT PTHMPTILDE PGKKVKKSAP LQSLTTSPTT
560 570 580 590 600
SQGSPGTGES RSQRPGSWAS RDTIFSTSPK LLARAITNGH RLKGEGSGVD
610 620 630 640 650
LEKGDSSSSS PEHSASSDPA KAPQTAESRA AHACDSQGTN CPTAGHPKAL
660 670 680 690 700
LNGVDAKMVK LKSPALSSTT TEPTSLMSPP PAKKLALSAK KASTLRRATG
710 720 730 740 750
NDIGSPSPSA FCDLTSPMKA THPVVASTGP VSKTRTAAPA PRPSTHPHSA
760 770 780 790 800
SLSSSSAKPL GTSEPQSCRP SAWTPLPQVN GHFTSHLHQL PEASEALHSP
810 820 830 840 850
SKKRKKTPNG DPQRLGIDTL LPQCLRGAPA AARRKRKKRC SEGEGATAPK
860 870 880 890 900
QEGQFQDQSW SSGSQKEEGT QPQVNGHQVS HILDSYHVSS RKRRKRKRSE
910 920 930 940 950
GLSQEATPSQ DLIQHSCSPV DHSEPEARTE LQKKKKKKRR KRKPEPQQDE
960 970 980 990 1000
ESKHPGDQRS PRPSVTPVPA LSVNGHLPSD CLGLGQAPLV TWNRDQEPDV
1010 1020 1030 1040 1050
VQALLQDSSD KAYGKKVLTW DGEPSAISQD AIKDSRLART QTVVDDWDEE
1060 1070 1080 1090
FDRGKEKKIK KFKREKKRNF NAFQKLQSRR NFWSVTHPAK VASLSYRR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB0QZF8 | B0QZF8_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp36 | 890 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 452 | S → P in BAC98173 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 486 | V → I in BAC98173 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 541 | L → P in BAC98173 (PubMed:14621295).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL591404 Genomic DNA No translation available. AK129363 mRNA Translation: BAC98173.1 Different initiation. |
CCDSi | CCDS48998.1 |
RefSeqi | NP_001028700.1, NM_001033528.1 XP_006534365.1, XM_006534302.3 XP_006534366.1, XM_006534303.2 XP_006534367.1, XM_006534304.3 |
Genome annotation databases
Ensembli | ENSMUST00000092382; ENSMUSP00000090036; ENSMUSG00000033909 ENSMUST00000106296; ENSMUSP00000101903; ENSMUSG00000033909 |
GeneIDi | 72344 |
KEGGi | mmu:72344 |
UCSCi | uc007mot.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL591404 Genomic DNA No translation available. AK129363 mRNA Translation: BAC98173.1 Different initiation. |
CCDSi | CCDS48998.1 |
RefSeqi | NP_001028700.1, NM_001033528.1 XP_006534365.1, XM_006534302.3 XP_006534366.1, XM_006534303.2 XP_006534367.1, XM_006534304.3 |
3D structure databases
SMRi | B1AQJ2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 215324, 1 interactor |
STRINGi | 10090.ENSMUSP00000101903 |
PTM databases
iPTMneti | B1AQJ2 |
PhosphoSitePlusi | B1AQJ2 |
Proteomic databases
EPDi | B1AQJ2 |
MaxQBi | B1AQJ2 |
PaxDbi | B1AQJ2 |
PeptideAtlasi | B1AQJ2 |
PRIDEi | B1AQJ2 |
ProteomicsDBi | 298360 |
Protocols and materials databases
Antibodypediai | 1709, 239 antibodies from 29 providers |
DNASUi | 72344 |
Genome annotation databases
Ensembli | ENSMUST00000092382; ENSMUSP00000090036; ENSMUSG00000033909 ENSMUST00000106296; ENSMUSP00000101903; ENSMUSG00000033909 |
GeneIDi | 72344 |
KEGGi | mmu:72344 |
UCSCi | uc007mot.2, mouse |
Organism-specific databases
CTDi | 57602 |
MGIi | MGI:1919594, Usp36 |
VEuPathDBi | HostDB:ENSMUSG00000033909 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG1865, Eukaryota |
GeneTreei | ENSGT00940000157948 |
HOGENOMi | CLU_008279_10_1_1 |
InParanoidi | B1AQJ2 |
OMAi | SETCLPQ |
OrthoDBi | 929408at2759 |
PhylomeDBi | B1AQJ2 |
TreeFami | TF315281 |
Miscellaneous databases
BioGRID-ORCSi | 72344, 24 hits in 66 CRISPR screens |
ChiTaRSi | Usp36, mouse |
PROi | PR:B1AQJ2 |
RNActi | B1AQJ2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000033909, Expressed in spleen and 285 other tissues |
ExpressionAtlasi | B1AQJ2, baseline and differential |
Genevisiblei | B1AQJ2, MM |
Family and domain databases
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF00443, UCH, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UBP36_MOUSE | |
Accessioni | B1AQJ2Primary (citable) accession number: B1AQJ2 Secondary accession number(s): Q6ZPQ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 7, 2009 |
Last sequence update: | April 8, 2008 | |
Last modified: | February 23, 2022 | |
This is version 101 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families