UniProtKB - B1AHB0 (B1AHB0_HUMAN)
Protein
DNA helicase
Gene
MCM5
Organism
Homo sapiens (Human)
Status
Functioni
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.ARBA annotation
Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.ARBA annotation
Catalytic activityi
- EC:3.6.4.12ARBA annotation
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-EC
- DNA replication origin binding Source: InterPro
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- DNA replication initiation Source: InterPro
Keywordsi
Molecular function | DNA-bindingUniRule annotationARBA annotation, HelicaseARBA annotation, Hydrolase |
Biological process | Cell cycle, Cell divisionImported, DNA replicationARBA annotation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:MCM5Imported ORF Names:hCG_41525Imported |
Organismi | Homo sapiens (Human)Imported |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Organism-specific databases
VEuPathDBi | HostDB:ENSG00000100297.15 |
Subcellular locationi
Nucleus
- Nucleus ARBA annotation
Other locations
- Chromosome ARBA annotation
Nucleus
- nucleoplasm Source: HPA
Other locations
- MCM complex Source: InterPro
Keywords - Cellular componenti
ChromosomeARBA annotation, NucleusARBA annotationExpressioni
Gene expression databases
Bgeei | ENSG00000100297, Expressed in bone marrow and 208 other tissues |
ExpressionAtlasi | B1AHB0, baseline and differential |
Interactioni
Binary interactionsi
Hide detailsB1AHB0
With | #Exp. | IntAct |
---|---|---|
KRTAP10-7 [P60409] | 3 | EBI-10175425,EBI-10172290 |
RBPMS [Q93062] | 3 | EBI-10175425,EBI-740322 |
Protein-protein interaction databases
IntActi | B1AHB0, 4 interactors |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 331 – 536 | MCMInterPro annotationAdd BLAST | 206 |
Sequence similaritiesi
Phylogenomic databases
OMAi | PRKCTTE |
OrthoDBi | 266497at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008048, MCM5 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01661, MCMPROTEIN5 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
B1AHB0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG
60 70 80 90 100
FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLYK QPAEHLQLLE
110 120 130 140 150
EAAKEVADEV TRPRPSGEEV LQDIQVMLKS DASPSSIRSL KSDMMSHLVK
160 170 180 190 200
IPGIIIAASA VRAKATRISI QCRSCRNTLT NIAMRPGLEG YALPRKCNTD
210 220 230 240 250
QAGRPKCPLD PYFIMPDKCK CVDFQTLKLQ ELPDAVPHGE MPRHMQLYCD
260 270 280 290 300
RYLCDKVVPG NRVTIMGIYS IKKFGLTTSR GRDRVGVGIR SSYIRVLGIQ
310 320 330 340 350
VDTDGSGRSF AGAVSPQEEE EFRRLAALPN VYEVISKSIA PSIFGGTDMK
360 370 380 390 400
KAIACLLFGG SRKRLPDGLT RRGDINLLML GDPGTAKSQL LKFVEKCSPI
410 420 430 440 450
GVYTSGKGSS AAGLTASVMR DPSSRNFIME GGAMVLADGG VVCIDEFDKM
460 470 480 490 500
REDDRVAIHE AMEQQTISIA KAGITTTLNS RCSVLAAANS VFGRWDETKG
510 520 530 540 550
EDNIDFMPTI LSRFDMIFIV KDEHNEERDV MLAKHVITLH VSALTQTQAV
560 570 580 590 600
EGEIDLAKLK KFIAYCRVKC GPRLSAEAAE KLKNRYIIMR SGARQHERDS
610 620 630 640 650
DRRSSIPITV RQLEAIVRIA EALSKMKLQP FATEADVEEA LRLFQVSTLD
660 670 680 690 700
AALSGTLSGV EGFTSQEDQE MLSRIEKQLK RRFAIGSQVS EHSIIKDFTK
710 720 730
QKYPEHAIHK VLQLMLRRGE IQHRMQRKVL YRLK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK312533 mRNA Translation: BAG35432.1 CH471095 Genomic DNA Translation: EAW60060.1 CH471095 Genomic DNA Translation: EAW60063.1 |
RefSeqi | NP_006730.2, NM_006739.3 |
Genome annotation databases
GeneIDi | 4174 |
KEGGi | hsa:4174 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK312533 mRNA Translation: BAG35432.1 CH471095 Genomic DNA Translation: EAW60060.1 CH471095 Genomic DNA Translation: EAW60063.1 |
RefSeqi | NP_006730.2, NM_006739.3 |
3D structure databases
SMRi | B1AHB0 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | B1AHB0, 4 interactors |
Proteomic databases
PRIDEi | B1AHB0 |
Protocols and materials databases
Antibodypediai | 244, 880 antibodies |
DNASUi | 4174 |
Genome annotation databases
GeneIDi | 4174 |
KEGGi | hsa:4174 |
Organism-specific databases
CTDi | 4174 |
PharmGKBi | PA30695 |
VEuPathDBi | HostDB:ENSG00000100297.15 |
Phylogenomic databases
OMAi | PRKCTTE |
OrthoDBi | 266497at2759 |
Miscellaneous databases
BioGRID-ORCSi | 4174, 746 hits in 882 CRISPR screens |
ChiTaRSi | MCM5, human |
GenomeRNAii | 4174 |
Gene expression databases
Bgeei | ENSG00000100297, Expressed in bone marrow and 208 other tissues |
ExpressionAtlasi | B1AHB0, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008048, MCM5 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01661, MCMPROTEIN5 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | B1AHB0_HUMAN | |
Accessioni | B1AHB0Primary (citable) accession number: B1AHB0 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | April 8, 2008 |
Last sequence update: | April 8, 2008 | |
Last modified: | February 10, 2021 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |