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Protein

ATPase get3

Gene

get3

Organism
Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63UniRule annotation1
Binding sitei245ATPUniRule annotation1
Binding sitei272ATPUniRule annotation1
Metal bindingi283Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi286Zinc; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 41ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase get3UniRule annotation (EC:3.6.-.-UniRule annotation)
Alternative name(s):
Arsenical pump-driving ATPaseUniRule annotation
Arsenite-stimulated ATPaseUniRule annotation
Golgi to ER traffic protein 3UniRule annotation
Guided entry of tail-anchored proteins 3UniRule annotation
Gene namesi
Name:get3
ORF Names:AFUB_037830
OrganismiNeosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus)
Taxonomic identifieri451804 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000001699 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003881901 – 340ATPase get3Add BLAST340

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0XXL5
SMRiB0XXL5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the arsA ATPase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000197637
OrthoDBiEOG092C33XF
PhylomeDBiB0XXL5

Family and domain databases

CDDicd02035 ArsA, 1 hit
HAMAPiMF_03112 Asna1_Get3, 1 hit
InterProiView protein in InterPro
IPR025723 Anion-transp_ATPase-like_dom
IPR016300 ATPase_ArsA/GET3
IPR027542 ATPase_ArsA/GET3_euk
IPR027417 P-loop_NTPase
PANTHERiPTHR10803 PTHR10803, 1 hit
PTHR10803:SF3 PTHR10803:SF3, 1 hit
PfamiView protein in Pfam
PF02374 ArsA_ATPase, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00345 GET3_arsA_TRC40, 1 hit

Sequencei

Sequence statusi: Complete.

B0XXL5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSTAVVHGD DLMEPTLQSI LSQKTLRWIF VGGKGGVGKT TTSCSLAIQL
60 70 80 90 100
AKVRKSVLLI STDPAHNLSD AFGQKFGKEA RLVDGYSNLS AMEIDPNGSI
110 120 130 140 150
QDLLASGDSQ GDDPLAGLGM GNMMQDLAFS IPGVDEAMSF AEVLKQVKSL
160 170 180 190 200
SYEVIVFDTA PTGHTLRFLQ FPTVLEKALA KLSQLSSQFG PMLNSILGAR
210 220 230 240 250
GGLPGGQNID ELLQKMESLR ETISEVNTQF KNPDMTTFVC VCIAEFLSLY
260 270 280 290 300
ETERMIQELT SYGIDTHAIV VNQLLFPKEG SGCEQCNARR KMQKKYLEQI
310 320 330 340
EELYEDFNVV RMPLLVEEVR GKEKLEKFSE MLVHPYVPPQ
Length:340
Mass (Da):37,440
Last modified:April 8, 2008 - v1
Checksum:i9F6B277957FFE461
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS499596 Genomic DNA Translation: EDP52617.1

Genome annotation databases

EnsemblFungiiEDP52617; EDP52617; AFUB_037830

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS499596 Genomic DNA Translation: EDP52617.1

3D structure databases

ProteinModelPortaliB0XXL5
SMRiB0XXL5
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEDP52617; EDP52617; AFUB_037830

Phylogenomic databases

HOGENOMiHOG000197637
OrthoDBiEOG092C33XF
PhylomeDBiB0XXL5

Family and domain databases

CDDicd02035 ArsA, 1 hit
HAMAPiMF_03112 Asna1_Get3, 1 hit
InterProiView protein in InterPro
IPR025723 Anion-transp_ATPase-like_dom
IPR016300 ATPase_ArsA/GET3
IPR027542 ATPase_ArsA/GET3_euk
IPR027417 P-loop_NTPase
PANTHERiPTHR10803 PTHR10803, 1 hit
PTHR10803:SF3 PTHR10803:SF3, 1 hit
PfamiView protein in Pfam
PF02374 ArsA_ATPase, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00345 GET3_arsA_TRC40, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGET3_ASPFC
AccessioniPrimary (citable) accession number: B0XXL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: April 8, 2008
Last modified: October 10, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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