We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - B0XMT4 (MNS1B_ASPFC)
Protein
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
Gene
mns1B
Organism
Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus)
Status
Functioni
Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 (By similarity).
By similarityCatalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
Cofactori
Ca2+By similarity, Mg2+By similarityNote: Ca2+. Can also use Mg2+, but with lower efficiency.By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 367 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B (EC:3.2.1.113By similarity)Alternative name(s): Class I alpha-mannosidase 1B Man(9)-alpha-mannosidase 1B |
Gene namesi | Name:mns1B Synonyms:msdS ORF Names:AFUB_014090 |
Organismi | Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus) |
Taxonomic identifieri | 451804 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:AFUB_014090 |
Subcellular locationi
Other locations
- Cytoplasmic vesicle lumen By similarity
Other locations
- cytoplasmic vesicle lumen Source: UniProtKB-SubCell
- membrane Source: InterPro
Keywords - Cellular componenti
Cytoplasmic vesiclePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000394817 | 19 – 493 | Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1BAdd BLAST | 475 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 87 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 174 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 324 ↔ 353 | By similarity | ||
Glycosylationi | 489 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinInteractioni
Subunit structurei
Monomer.
By similarityFamily & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
HOGENOMi | CLU_003818_0_2_1 |
PhylomeDBi | B0XMT4 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
B0XMT4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHLPSLSVAL ALVSSSLALP QAVLPENDVS SRAAAVKEAF SHAWDGYMKY
60 70 80 90 100
AFPHDELLPV SNSYGDSRNG WGASAVDALS TAIVMRNATI VSQILDHIAK
110 120 130 140 150
IDYSKTSDMV SLFETTIRYL GGMLSGYDLL KGPAADLVED RTKVDMLLQQ
160 170 180 190 200
SKNLGDVLKF AFDTPSGVPY NNINITSHGN DGATTNGLAV TGTLVLEWTR
210 220 230 240 250
LSDLTGDQEY AKLSQRAESY LLAPQPSSGE PFPGLVGSAI SIQTGQFTNG
260 270 280 290 300
FVSWNGGSDS FYEYLMKMYV YDPKRFATYK DRWVAAAESS IDHLASNPAS
310 320 330 340 350
RPDLTFLATY NKGSLGLSSQ HLACFDGGSY LLGGTVLDRA DLIDFGLKLV
360 370 380 390 400
DGCAETYHQT LTGIGPESFG WDEKSVPADQ KELYERAGFY VQSGAYILRP
410 420 430 440 450
EVIESFYYAY RVTGKKQYRD WVWNAFENIN KYCRTESGFA GLTNVNAVNG
460 470 480 490
GGRYDNQESF LFAEVMKYAY LTHAPGMSSM PAAAEDKANK SRG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DS499594 Genomic DNA Translation: EDP56691.1 |
Genome annotation databases
EnsemblFungii | EDP56691; EDP56691; AFUB_014090 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DS499594 Genomic DNA Translation: EDP56691.1 |
3D structure databases
SMRi | B0XMT4 |
ModBasei | Search... |
Protein family/group databases
Allergomei | 8990, Asp f Mannosidase |
Genome annotation databases
EnsemblFungii | EDP56691; EDP56691; AFUB_014090 |
Organism-specific databases
VEuPathDBi | FungiDB:AFUB_014090 |
Phylogenomic databases
HOGENOMi | CLU_003818_0_2_1 |
PhylomeDBi | B0XMT4 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MNS1B_ASPFC | |
Accessioni | B0XMT4Primary (citable) accession number: B0XMT4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 15, 2010 |
Last sequence update: | April 8, 2008 | |
Last modified: | September 29, 2021 | |
This is version 60 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Documents
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families