UniProtKB - B0U0S3 (BIOB_FRAP2)
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Protein
Biotin synthase
Gene
bioB
Organism
Francisella philomiragia subsp. philomiragia (strain ATCC 25017)
Status
Functioni
Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation
Catalytic activityi
Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation
Cofactori
Protein has several cofactor binding sites:- [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
- [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation
: biotin biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- ATP-dependent dethiobiotin synthetase BioD (bioD)
- Biotin synthase (bioB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 46 | Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 50 | Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 53 | Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 90 | Iron-sulfur 2 (2Fe-2S)UniRule annotation | 1 | |
Metal bindingi | 121 | Iron-sulfur 2 (2Fe-2S)UniRule annotation | 1 | |
Metal bindingi | 181 | Iron-sulfur 2 (2Fe-2S)UniRule annotation | 1 | |
Metal bindingi | 256 | Iron-sulfur 2 (2Fe-2S)UniRule annotation | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- biotin synthase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- biotin biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Biotin biosynthesis |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine |
Enzyme and pathway databases
UniPathwayi | UPA00078; UER00162. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:bioBUniRule annotation Ordered Locus Names:Fphi_1799 |
Organismi | Francisella philomiragia subsp. philomiragia (strain ATCC 25017) |
Taxonomic identifieri | 484022 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000381387 | 1 – 313 | Biotin synthaseAdd BLAST | 313 |
Interactioni
Subunit structurei
Homodimer.UniRule annotation
Protein-protein interaction databases
STRINGi | 484022.Fphi_1799. |
Structurei
3D structure databases
ProteinModelPortali | B0U0S3. |
SMRi | B0U0S3. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105CZF. Bacteria. COG0502. LUCA. |
HOGENOMi | HOG000239957. |
KOi | K01012. |
OMAi | ADRFCMG. |
OrthoDBi | POG091H01DF. |
Family and domain databases
Gene3Di | 3.20.20.70. 1 hit. |
HAMAPi | MF_01694. BioB. 1 hit. |
InterProi | View protein in InterPro IPR013785. Aldolase_TIM. IPR010722. BATS_dom. IPR034416. BATS_domain_containing. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. |
PANTHERi | PTHR22976. PTHR22976. 1 hit. |
Pfami | View protein in Pfam PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. |
PIRSFi | PIRSF001619. Biotin_synth. 1 hit. |
SFLDi | SFLDF00272. biotin_synthase. 1 hit. SFLDG01060. BATS_domain_containing. 1 hit. SFLDG01278. biotin_synthase_like. 1 hit. SFLDS00029. Radical_SAM. 1 hit. |
SMARTi | View protein in SMART SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. |
TIGRFAMsi | TIGR00433. bioB. 1 hit. |
i Sequence
Sequence statusi: Complete.
B0U0S3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLEQIKEIY SRPLTELILQ ALEIHNKNFG NDIELCSLKS IKTGTCPEDC
60 70 80 90 100
KYCPQSGHYN TSIEKHKLLD KDSILAEAKN AKDAGSKRFC MGAAWKHIPK
110 120 130 140 150
KDFDQVAEII TEVKNLGLET CVTLGSINAE EATKLKEAGL DYYNHNLDTS
160 170 180 190 200
REFYPEIITT RKFEERIETI RNVANADINV CCGGILGMGE SLDDRFNLLL
210 220 230 240 250
ELLQLPAAPK SIPINTLIPV KGTPLGDKYT DAQIDSFELV RFIATTRILF
260 270 280 290 300
PQARLRLSAG RENMSLETQT LCFLAGINSI FYGNKLLTEN NATINSDNFL
310
LAKLGLESNV ELC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000937 Genomic DNA. Translation: ABZ88026.1. |
RefSeqi | WP_012281076.1. NC_010336.1. |
Genome annotation databases
EnsemblBacteriai | ABZ88026; ABZ88026; Fphi_1799. |
GeneIDi | 29665683. |
KEGGi | fph:Fphi_1799. |
Similar proteinsi
Entry informationi
Entry namei | BIOB_FRAP2 | |
Accessioni | B0U0S3Primary (citable) accession number: B0U0S3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 28, 2009 |
Last sequence update: | April 8, 2008 | |
Last modified: | November 22, 2017 | |
This is version 63 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |