bioB

Functionⁱ

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityⁱ

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactorⁱ

Protein has several cofactor binding sites:

[4Fe-4S] cluster
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694
[2Fe-2S] cluster
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694
Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694

Pathwayⁱ: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

ATP-dependent dethiobiotin synthetase BioD (bioD)
Biotin synthase (bioB)

This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	46	Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation	1
Metal bindingⁱ	50	Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation	1
Metal bindingⁱ	53	Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation	1
Metal bindingⁱ	90	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1
Metal bindingⁱ	121	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1
Metal bindingⁱ	181	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1
Metal bindingⁱ	256	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1

GO - Molecular functionⁱ

2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
biotin synthase activity Source: UniProtKB-EC
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

biotin biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Biotin biosynthesis
Ligand	2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayⁱ	UPA00078; UER00162.

UniPathwayⁱ

UPA00078; UER00162.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesⁱ	Name:bioBUniRule annotation Ordered Locus Names:Fphi_1799
Organismⁱ	Francisella philomiragia subsp. philomiragia (strain ATCC 25017)
Taxonomic identifierⁱ	484022 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella philomiragia
Proteomesⁱ	UP000001320 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000381387	1 – 313	Biotin synthaseAdd BLAST		313

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	484022.Fphi_1799.

STRINGⁱ

484022.Fphi_1799.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	B0U0S3.
SMRⁱ	B0U0S3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105CZF. Bacteria. COG0502. LUCA.
HOGENOMⁱ	HOG000239957.
KEGG Orthology (KO) More...KOⁱ	K01012.
OMAⁱ	ADRFCMG.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01DF.

Family and domain databases

Gene3Dⁱ	3.20.20.70. 1 hit.
HAMAPⁱ	MF_01694. BioB. 1 hit.
InterProⁱ	View protein in InterPro IPR013785. Aldolase_TIM. IPR010722. BATS_dom. IPR034416. BATS_domain_containing. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM.
PANTHERⁱ	PTHR22976. PTHR22976. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit.
PIRSFⁱ	PIRSF001619. Biotin_synth. 1 hit.
SFLDⁱ	SFLDF00272. biotin_synthase. 1 hit. SFLDG01060. BATS_domain_containing. 1 hit. SFLDG01278. biotin_synthase_like. 1 hit. SFLDS00029. Radical_SAM. 1 hit.
SMARTⁱ	View protein in SMART SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit.
TIGRFAMsⁱ	TIGR00433. bioB. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

B0U0S3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTLEQIKEIY SRPLTELILQ ALEIHNKNFG NDIELCSLKS IKTGTCPEDC 
        60         70         80         90        100
KYCPQSGHYN TSIEKHKLLD KDSILAEAKN AKDAGSKRFC MGAAWKHIPK 
       110        120        130        140        150
KDFDQVAEII TEVKNLGLET CVTLGSINAE EATKLKEAGL DYYNHNLDTS 
       160        170        180        190        200
REFYPEIITT RKFEERIETI RNVANADINV CCGGILGMGE SLDDRFNLLL 
       210        220        230        240        250
ELLQLPAAPK SIPINTLIPV KGTPLGDKYT DAQIDSFELV RFIATTRILF 
       260        270        280        290        300
PQARLRLSAG RENMSLETQT LCFLAGINSI FYGNKLLTEN NATINSDNFL 
       310 
LAKLGLESNV ELC

Length:313

Mass (Da):34,941

Last modified:April 8, 2008 - v1

Checksum:ⁱ60611F2E05CA2709

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000937 Genomic DNA. Translation: ABZ88026.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_012281076.1. NC_010336.1.

Genome annotation databases

EnsemblBacteriaⁱ	ABZ88026; ABZ88026; Fphi_1799.
GeneIDⁱ	29665683.
KEGGⁱ	fph:Fphi_1799.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
B0U0S3	A0A248KNI0 A0A0E2ZJZ1	Francisella noatunensis subsp. noatunensis FSC772 Francisella philomiragia	313	UniRef100_B0U0S3	Cluster: Biotin synthase	3

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
B0U0S3	A0A248KNI0 A0A0E2ZJZ1 Q14HR4 Q5NGB2 A0A0E2ZGR6 A0A0H3TQB1 A7NCW7 Q2A2V9 Q0BLD5 A0A0B3VR27 +21	Francisella noatunensis subsp. noatunensis FSC772 Francisella philomiragia Francisella tularensis subsp. tularensis (strain FSC 198) Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) Francisella tularensis Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) Francisella tularensis subsp. holarctica (strain LVS) Francisella tularensis subsp. holarctica (strain OSU18) Francisella tularensis subsp. holarctica Francisella tularensis subsp. mediasiatica (strain FSC147) And more	313	UniRef90_B0U0S3	Cluster: Biotin synthase	32

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
B0U0S3	Q3YRG6 A0A0C7D8F2 A0A1F8J5H1 K2EJ46 D0X1G8 A0A1J0KG92 A0Q638 A4IXP5 A0A0B6KKY5 A7NCW7 +40	Ehrlichia canis (strain Jake) Ehrlichia minasensis Chlamydiae bacterium RIFCSPHIGHO2_01_FULL_44_39 groundwater metagenome Vibrio alginolyticus 40B Francisella sp. MA067296 Francisella tularensis subsp. novicida (strain U112) Francisella tularensis subsp. tularensis (strain WY96-3418) Francisella novicida Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) And more	324	UniRef50_Q3YRG6	Cluster: Biotin synthase	51

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000937 Genomic DNA. Translation: ABZ88026.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_012281076.1. NC_010336.1.

3D structure databases

ProteinModelPortalⁱ	B0U0S3.
SMRⁱ	B0U0S3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	484022.Fphi_1799.

STRINGⁱ

484022.Fphi_1799.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ABZ88026; ABZ88026; Fphi_1799.
GeneIDⁱ	29665683.
KEGGⁱ	fph:Fphi_1799.

Phylogenomic databases

eggNOGⁱ	ENOG4105CZF. Bacteria. COG0502. LUCA.
HOGENOMⁱ	HOG000239957.
KEGG Orthology (KO) More...KOⁱ	K01012.
OMAⁱ	ADRFCMG.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01DF.

Enzyme and pathway databases

UniPathwayⁱ	UPA00078; UER00162.

UniPathwayⁱ

UPA00078; UER00162.

Family and domain databases

Gene3Dⁱ	3.20.20.70. 1 hit.
HAMAPⁱ	MF_01694. BioB. 1 hit.
InterProⁱ	View protein in InterPro IPR013785. Aldolase_TIM. IPR010722. BATS_dom. IPR034416. BATS_domain_containing. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM.
PANTHERⁱ	PTHR22976. PTHR22976. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit.
PIRSFⁱ	PIRSF001619. Biotin_synth. 1 hit.
SFLDⁱ	SFLDF00272. biotin_synthase. 1 hit. SFLDG01060. BATS_domain_containing. 1 hit. SFLDG01278. biotin_synthase_like. 1 hit. SFLDS00029. Radical_SAM. 1 hit.
SMARTⁱ	View protein in SMART SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit.
TIGRFAMsⁱ	TIGR00433. bioB. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BIOB_FRAP2
Accessionⁱ	B0U0S3Primary (citable) accession number: B0U0S3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
	Last sequence update:	April 8, 2008
	Last modified:	November 22, 2017
	This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

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UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - B0U0S3 (BIOB_FRAP2)

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Biotin synthase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: biotin biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

Pathwayⁱ: biotin biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ