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Protein

Thymidylate synthase

Gene

thyA

Organism
Francisella philomiragia subsp. philomiragia (strain ATCC 25017)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Binding sitei515,10-methylenetetrahydrofolateUniRule annotation1
Active sitei156NucleophileUniRule annotation1
Binding sitei1795,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei187dUMPUniRule annotation1
Binding sitei2735,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi123 – 124dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi176 – 179dUMPUniRule annotation4
Nucleotide bindingi217 – 219dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:Fphi_1432
OrganismiFrancisella philomiragia subsp. philomiragia (strain ATCC 25017)
Taxonomic identifieri484022 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
Proteomesi
  • UP000001320 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000738761 – 274Thymidylate synthaseAdd BLAST274

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi484022.Fphi_1432

Structurei

3D structure databases

ProteinModelPortaliB0TYI1
SMRiB0TYI1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257899
KOiK00560
OMAiKQYLDLC
OrthoDBiPOG091H02L6

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.101 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 2 hits

Sequencei

Sequence statusi: Complete.

B0TYI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYLNFLKY IKDNGTKKGD RTGTGTTSIF GYQMRFDLQQ GFPLVTTKKI
60 70 80 90 100
HIPSVVHELL WFLSGSTNVK YLNDNKVRIW NEWATEEGEL GPIYGKQWRD
110 120 130 140 150
FNGEGIDQIA EVIEMLKTNP NSRRILVSAW NPCVVPSEKI SPQENVAKGN
160 170 180 190 200
SALPPCHAMF QFYIADNKLS CMLTQRSADA FLGVPFNIAS YSLLTHMIAQ
210 220 230 240 250
QCDLDVGEFI WSGGDCHIYN NHIEQVNEQL SREPLDLPTL KILRKPSSIF
260 270
DYKYEDFEFQ NYKHHPAIKA KISV
Length:274
Mass (Da):31,416
Last modified:April 8, 2008 - v1
Checksum:iBA7F08EBEAC072E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000937 Genomic DNA Translation: ABZ87657.1
RefSeqiWP_012280776.1, NC_010336.1

Genome annotation databases

EnsemblBacteriaiABZ87657; ABZ87657; Fphi_1432
GeneIDi29665972
KEGGifph:Fphi_1432

Similar proteinsi

Entry informationi

Entry nameiTYSY_FRAP2
AccessioniPrimary (citable) accession number: B0TYI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: October 25, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome