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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi299Magnesium or manganese 1UniRule annotation1
Metal bindingi313Magnesium or manganese 1UniRule annotation1
Metal bindingi313Magnesium or manganese 2UniRule annotation1
Metal bindingi315Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi172 – 227ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:Teth39_0187
OrganismiThermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
Taxonomic identifieri340099 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
Proteomesi
  • UP000002156 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003411841 – 359D-alanine--D-alanine ligaseAdd BLAST359

Proteomic databases

PRIDEiB0KBI1

Interactioni

Protein-protein interaction databases

STRINGi340099.Teth39_0187

Structurei

3D structure databases

ProteinModelPortaliB0KBI1
SMRiB0KBI1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 346ATP-graspUniRule annotationAdd BLAST206

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011593
KOiK01921
OMAiYETKYTE

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

B0KBI1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKKKIAVLF GGQSGEHEVS LMSAKSIINN LDKDKYEIYM VGITKKGEWY
60 70 80 90 100
LYRGDVGKIE TGEWEKEGIP AIMGASTKYR GIITFEDGEN GFYPIDVVFP
110 120 130 140 150
VLHGPNGEDG TIQGLLELLD MPYVGANVLS SALCMDKVFT KRIFKEAGLP
160 170 180 190 200
TPDFVVVYGK EIEDLEAIKK KIEHLGYPCF VKPANLGSSV GITKVHNEEE
210 220 230 240 250
LPGALKLAAK YDRKLLIERG IDAREIECSV LGNENPEASI AGEIVPSNEF
260 270 280 290 300
YDYNAKYFDG GKSLLLIPAP LPDEKMEEVR KLAIKAYKAL DLRGMARVDF
310 320 330 340 350
LMDRNTGTLY LNEVNTIPGF TKISMYPKLW ESSGKSYSTL LDELINLAVE

SHNEKCREW
Length:359
Mass (Da):40,083
Last modified:March 18, 2008 - v1
Checksum:iA8646128A86188AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000924 Genomic DNA Translation: ABY93860.1
RefSeqiWP_009052136.1, NC_010321.1

Genome annotation databases

EnsemblBacteriaiABY93860; ABY93860; Teth39_0187
KEGGitpd:Teth39_0187

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000924 Genomic DNA Translation: ABY93860.1
RefSeqiWP_009052136.1, NC_010321.1

3D structure databases

ProteinModelPortaliB0KBI1
SMRiB0KBI1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi340099.Teth39_0187

Proteomic databases

PRIDEiB0KBI1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY93860; ABY93860; Teth39_0187
KEGGitpd:Teth39_0187

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011593
KOiK01921
OMAiYETKYTE

Enzyme and pathway databases

UniPathwayi
UPA00219

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDDL_THEP3
AccessioniPrimary (citable) accession number: B0KBI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 18, 2008
Last modified: May 23, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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