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Entry version 94 (12 Aug 2020)
Sequence version 1 (26 Feb 2008)
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Protein

Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrial

Gene

BIO3-BIO1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Bifunctional enzyme that catalyzes two different reactions involved in the biotin biosynthesis.4 Publications
Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring.By similarity5 Publications
Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Absorptioni

Abs(max)=415 nm1 PublicationShoulder at 335 nm (at pH 7.5 and 30 degrees Celsius).1 Publication

Kineticsi

kcat is 0.072 min(-1) for 7,8-diamino-pelargonic acid aminotransferase + dethiobiotin synthetase activities, and 1.85 min(-1) for 7,8-diamino-pelargonic acid aminotransferase activity only (at pH 7.5 and 30 degrees Celsius).1 Publication

      <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: biotin biosynthesis

      This protein is involved in step 1 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.1 Publication
      Proteins known to be involved in the 2 steps of the subpathway in this organism are:
      1. Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrial (BIO3-BIO1), Dethiobiotin synthase (AXX17_At5g56870)
      2. Biotin synthase (At2g43360), Biotin synthase (AXX17_At2g40830), Biotin synthase, mitochondrial (BIO2), Biotin synthase
      This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
      View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

      Pathwayi: biotin biosynthesis

      This protein is involved in step 1 of the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route).1 Publication
      Proteins known to be involved in this subpathway in this organism are:
      1. Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrial (BIO3-BIO1)
      This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
      View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route), the pathway biotin biosynthesis and in Cofactor biosynthesis.

      Sites

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi51MagnesiumCombined sources1 Publication1
      <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei81SubstrateCombined sources1 Publication1
      Metal bindingi88MagnesiumCombined sources1 Publication1
      Binding sitei97ATPBy similarity1
      Metal bindingi210MagnesiumCombined sources1 Publication1
      <p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei348Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAMBy similarity1
      Binding sitei4957-keto-8-aminopelargonic acidCombined sources1 Publication1
      Binding sitei545ATPBy similarity1
      Binding sitei637Pyridoxal phosphateCombined sources1 Publication1
      Binding sitei6667-keto-8-aminopelargonic acidCombined sources1 Publication1
      Binding sitei7007-keto-8-aminopelargonic acid; via carbonyl oxygenCombined sources1 Publication1
      Binding sitei7977-keto-8-aminopelargonic acidCombined sources1 Publication1

      Regions

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi47 – 52ATPBy similarity6
      Nucleotide bindingi210 – 213ATPBy similarity4
      Nucleotide bindingi270 – 271ATPBy similarity2
      Nucleotide bindingi518 – 520ATPBy similarity3

      <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

      • adenosylmethionine-8-amino-7-oxononanoate transaminase activity Source: TAIR
      • ATP binding Source: UniProtKB-KW
      • dethiobiotin synthase activity Source: TAIR
      • magnesium ion binding Source: InterPro
      • protein homodimerization activity Source: UniProtKB
      • pyridoxal phosphate binding Source: InterPro

      GO - Biological processi

      • biotin biosynthetic process Source: TAIR

      <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

      Molecular functionAminotransferase, Ligase, Multifunctional enzyme, Transferase
      Biological processBiotin biosynthesis
      LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Pyridoxal phosphate, S-adenosyl-L-methionine

      Enzyme and pathway databases

      BioCyc Collection of Pathway/Genome Databases

      More...
      BioCyci
      MetaCyc:MONOMER-8566

      BRENDA Comprehensive Enzyme Information System

      More...
      BRENDAi
      2.6.1.62, 399
      6.3.3.3, 399

      UniPathway: a resource for the exploration and annotation of metabolic pathways

      More...
      UniPathwayi
      UPA00078;UER00160
      UPA00078;UER00161

      <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
      Recommended name:
      Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrial
      Alternative name(s):
      Bifunctional BIO3-BIO1 protein1 Publication
      Including the following 2 domains:
      Dethiobiotin synthetase (EC:6.3.3.31 Publication)
      Alternative name(s):
      DTB synthetase
      Short name:
      DTBS
      Protein BIOTIN AUXOTROPH 31 Publication
      7,8-diamino-pelargonic acid aminotransferase
      Short name:
      DAPA AT
      Short name:
      DAPA aminotransferase
      Alternative name(s):
      7,8-diaminononanoate synthase
      Short name:
      DANS
      Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC:2.6.1.621 Publication)
      Diaminopelargonic acid synthase
      Protein BIOTIN AUXOTROPH 11 Publication
      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
      Name:BIO3-BIO11 Publication
      Synonyms:BIO11 Publication, BIO31 Publication
      Ordered Locus Names:At5g57590Imported
      ORF Names:MUA2.17/MUA2.18Imported
      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArabidopsis thaliana (Mouse-ear cress)
      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3702 [NCBI]
      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
      <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
      • UP000006548 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 5

      Organism-specific databases

      Arabidopsis Information Portal

      More...
      Araporti
      AT5G57590

      The Arabidopsis Information Resource

      More...
      TAIRi
      locus:2174532, AT5G57590

      <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

      Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

      Keywords - Cellular componenti

      Mitochondrion

      <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

      <p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

      Arrested embryos at the transition to cotyledon stages of development (PubMed:11779812, PubMed:17993549). Biotin depletion leading to lethality; this phenotype is rescued by exogenous supply of biotin (PubMed:12644697, PubMed:23031218).4 Publications

      Mutagenesis

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi348F → Y: No important impact on the enzyme kinetic parameters. 1 Publication1
      Mutagenesisi382S → Y: Reduced substrate channeling leading to slower 7,8-diamino-pelargonic acid aminotransferase + dethiobiotin synthetase activities. 1 Publication1
      Mutagenesisi777 – 833Missing in bio1-1: Arrested embryo. 1 PublicationAdd BLAST57
      Mutagenesisi815I → W: Reduced substrate channeling leading to slower 7,8-diamino-pelargonic acid aminotransferase + dethiobiotin synthetase activities. 1 Publication1

      <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

      Molecule processing

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the 'PTM / Processing' section describes the extent of a transit peptide.<p><a href='/help/transit' target='_top'>More...</a></p>Transit peptidei1 – 23MitochondrionSequence analysisAdd BLAST23
      <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000041769624 – 833Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrialAdd BLAST810

      Amino acid modifications

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei666N6-(pyridoxal phosphate)lysineCombined sources1 Publication1

      Proteomic databases

      PaxDb, a database of protein abundance averages across all three domains of life

      More...
      PaxDbi
      B0F481

      PRoteomics IDEntifications database

      More...
      PRIDEi
      B0F481

      ProteomicsDB: a multi-organism proteome resource

      More...
      ProteomicsDBi
      240795 [B0F481-1]

      <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

      Gene expression databases

      ExpressionAtlas, Differential and Baseline Expression

      More...
      ExpressionAtlasi
      B0F481, baseline and differential

      Genevisible search portal to normalized and curated expression data from Genevestigator

      More...
      Genevisiblei
      B0F481, AT

      <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

      <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

      Homodimer.

      1 Publication

      GO - Molecular functioni

      • protein homodimerization activity Source: UniProtKB

      Protein-protein interaction databases

      STRING: functional protein association networks

      More...
      STRINGi
      3702.AT5G57590.1

      <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

      Secondary structure

      1833
      Legend: HelixTurnBeta strandPDB Structure known for this area
      Show more details

      3D structure databases

      SWISS-MODEL Repository - a database of annotated 3D protein structure models

      More...
      SMRi
      B0F481

      Database of comparative protein structure models

      More...
      ModBasei
      Search...

      Protein Data Bank in Europe - Knowledge Base

      More...
      PDBe-KBi
      Search...

      <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

      Region

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni36 – 299Dethiobiotin synthetaseCuratedAdd BLAST264
      Regioni332 – 8307,8-diamino-pelargonic acid aminotransferaseCuratedAdd BLAST499
      Regioni391 – 3927-keto-8-aminopelargonic acid bindingCombined sources1 Publication2
      Regioni453 – 454Pyridoxal phosphate bindingCombined sources1 Publication2
      Regioni701 – 702Pyridoxal phosphate bindingCombined sources1 Publication2

      <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

      In the N-terminal section; belongs to the dethiobiotin synthetase family.Curated
      In the C-terminal section; belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.Curated

      Keywords - Domaini

      Transit peptide

      Phylogenomic databases

      evolutionary genealogy of genes: Non-supervised Orthologous Groups

      More...
      eggNOGi
      KOG1401, Eukaryota

      The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

      More...
      HOGENOMi
      CLU_010794_0_0_1

      InParanoid: Eukaryotic Ortholog Groups

      More...
      InParanoidi
      B0F481

      KEGG Orthology (KO)

      More...
      KOi
      K19562

      Identification of Orthologs from Complete Genome Data

      More...
      OMAi
      KVEWYRG

      Database of Orthologous Groups

      More...
      OrthoDBi
      289012at2759

      Database for complete collections of gene phylogenies

      More...
      PhylomeDBi
      B0F481

      Family and domain databases

      Gene3D Structural and Functional Annotation of Protein Families

      More...
      Gene3Di
      3.40.640.10, 1 hit

      HAMAP database of protein families

      More...
      HAMAPi
      MF_00336, BioD, 1 hit

      Integrated resource of protein families, domains and functional sites

      More...
      InterProi
      View protein in InterPro
      IPR005814, Aminotrans_3
      IPR004472, DTB_synth_BioD
      IPR027417, P-loop_NTPase
      IPR015424, PyrdxlP-dep_Trfase
      IPR015421, PyrdxlP-dep_Trfase_major

      Pfam protein domain database

      More...
      Pfami
      View protein in Pfam
      PF00202, Aminotran_3, 1 hit

      Superfamily database of structural and functional annotation

      More...
      SUPFAMi
      SSF52540, SSF52540, 1 hit
      SSF53383, SSF53383, 1 hit

      PROSITE; a protein domain and family database

      More...
      PROSITEi
      View protein in PROSITE
      PS00600, AA_TRANSFER_CLASS_3, 1 hit

      <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (5)i

      <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

      <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

      This entry describes 5 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
      Note: Only the isoform BIO3-BIO1 is detected at protein level in mitochondria.1 Publication
      Isoform 1 (identifier: B0F481-1) [UniParc]FASTAAdd to basket
      Also known as: BIO3-BIO1

      This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

      « Hide
              10         20         30         40         50
      MIPVTATLIR HRLRHLRHRI RFKSTSVSPF HLPLNHPTYL IWSANTSLGK
      60 70 80 90 100
      TLVSTGIAAS FLLQQPSSSA TKLLYLKPIQ TGFPSDSDSR FVFSKLDSLS
      110 120 130 140 150
      LRRQIPISIS NSVLHSSLPA AKSLGLNVEV SESGMCSLNF RDEKTVTGAP
      160 170 180 190 200
      ELLCKTLYAW EAAISPHLAA ERENATVEDS VVLQMIEKCL KEEMECGVKS
      210 220 230 240 250
      EKSDLLCLVE TAGGVASPGP SGTLQCDLYR PFRLPGILVG DGRLGGISGT
      260 270 280 290 300
      IAAYESLKLR GYDIAAVVFE DHGLVNEVPL TSYLRNKVPV LVLPPVPKDP
      310 320 330 340 350
      SDDLIEWFVE SDGVFKALKE TMVLANLERL ERLNGMAKLA GEVFWWPFTQ
      360 370 380 390 400
      HKLVHQETVT VIDSRCGENF SIYKASDNSS LSQQFDACAS WWTQGPDPTF
      410 420 430 440 450
      QAELAREMGY TAARFGHVMF PENVYEPALK CAELLLDGVG KGWASRVYFS
      460 470 480 490 500
      DNGSTAIEIA LKMAFRKFCV DHNFCEATEE EKHIVVKVIA LRGSYHGDTL
      510 520 530 540 550
      GAMEAQAPSP YTGFLQQPWY TGRGLFLDPP TVFLSNGSWN ISLPESFSEI
      560 570 580 590 600
      APEYGTFTSR DEIFDKSRDA STLARIYSAY LSKHLQEHSG VRQSAHVGAL
      610 620 630 640 650
      IIEPVIHGAG GMHMVDPLFQ RVLVNECRNR KIPVIFDEVF TGFWRLGVET
      660 670 680 690 700
      TTELLGCKPD IACFAKLLTG GMVPLAVTLA TDAVFDSFSG DSKLKALLHG
      710 720 730 740 750
      HSYSAHAMGC ATAAKAIQWF KDPETNHNIT SQGKTLRELW DEELVQQISS
      760 770 780 790 800
      HSAVQRVVVI GTLFALELKA DASNSGYASL YAKSLLIMLR EDGIFTRPLG
      810 820 830
      NVIYLMCGPC TSPEICRRLL TKLYKRLGEF NRT
      Length:833
      Mass (Da):91,935
      Last modified:February 26, 2008 - v1
      <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i87A5D5C624E19649
      GO
      Isoform 2 (identifier: B0F481-2) [UniParc]FASTAAdd to basket
      Also known as: BIO3 long

      The sequence of this isoform differs from the canonical sequence as follows:
           403-405: ELA → VSG
           406-833: Missing.

      Note: May be due to a competing acceptor splice site.Curated
      Show »
      Length:405
      Mass (Da):44,488
      Checksum:i1716270B1AD0DEE2
      GO
      Isoform 3 (identifier: B0F481-3) [UniParc]FASTAAdd to basket
      Also known as: BIO1 short

      The sequence of this isoform differs from the canonical sequence as follows:
           1-382: Missing.
           383-399: QQFDACASWWTQGPDPT → MLVQAGGHRGQILLSRL

      Note: May be due to a competing acceptor splice site.Curated
      Show »
      Length:451
      Mass (Da):49,886
      Checksum:i0307A601E290A62A
      GO
      Isoform 4 (identifier: B0F481-4) [UniParc]FASTAAdd to basket
      Also known as: BIO3 short

      The sequence of this isoform differs from the canonical sequence as follows:
           288-833: Missing.

      Note: May be due to an intron retention.Curated
      Show »
      Length:287
      Mass (Da):31,265
      Checksum:i4D465F20B07E7160
      GO
      Isoform 5 (identifier: B0F481-5) [UniParc]FASTAAdd to basket
      Also known as: BIO1 long

      The sequence of this isoform differs from the canonical sequence as follows:
           1-321: Missing.

      Show »
      Length:512
      Mass (Da):56,926
      Checksum:iBC0C08B753FDD168
      GO

      <p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

      The sequence BAB08794 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g57590 and At5g57600.Curated
      The sequence BAB08795 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g57590 and At5g57600.Curated

      Experimental Info

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti236G → E in AAQ62434 (PubMed:14593172).Curated1

      Alternative sequence

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      <p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0438841 – 382Missing in isoform 3. 3 PublicationsAdd BLAST382
      Alternative sequenceiVSP_0438851 – 321Missing in isoform 5. CuratedAdd BLAST321
      Alternative sequenceiVSP_043886288 – 833Missing in isoform 4. 1 PublicationAdd BLAST546
      Alternative sequenceiVSP_043887383 – 399QQFDA…GPDPT → MLVQAGGHRGQILLSRL in isoform 3. 3 PublicationsAdd BLAST17
      Alternative sequenceiVSP_043888403 – 405ELA → VSG in isoform 2. 2 Publications3
      Alternative sequenceiVSP_043889406 – 833Missing in isoform 2. 2 PublicationsAdd BLAST428

      Sequence databases

      Select the link destinations:

      EMBL nucleotide sequence database

      More...
      EMBLi

      GenBank nucleotide sequence database

      More...
      GenBanki

      DNA Data Bank of Japan; a nucleotide sequence database

      More...
      DDBJi
      Links Updated
      EU089963 mRNA Translation: ABW80569.1
      EU090805 mRNA Translation: ABU50828.1
      EU090805 mRNA Translation: ABU50829.1
      EF081156 mRNA Translation: ABN80998.1
      HQ857557 mRNA Translation: AEW48251.1
      HQ857558 mRNA Translation: AEW48252.1
      AB011482 Genomic DNA Translation: BAB08794.1 Sequence problems.
      AB011482 Genomic DNA Translation: BAB08795.1 Sequence problems.
      CP002688 Genomic DNA Translation: AED96924.1
      BT010433 mRNA Translation: AAQ62434.1
      AK175602 mRNA Translation: BAD43365.1

      NCBI Reference Sequences

      More...
      RefSeqi
      NP_200567.2, NM_125140.4 [B0F481-1]

      Genome annotation databases

      Ensembl plant genome annotation project

      More...
      EnsemblPlantsi
      AT5G57590.1; AT5G57590.1; AT5G57590 [B0F481-1]

      Database of genes from NCBI RefSeq genomes

      More...
      GeneIDi
      835863

      Gramene; a comparative resource for plants

      More...
      Gramenei
      AT5G57590.1; AT5G57590.1; AT5G57590 [B0F481-1]

      KEGG: Kyoto Encyclopedia of Genes and Genomes

      More...
      KEGGi
      ath:AT5G57590

      Keywords - Coding sequence diversityi

      Alternative splicing

      <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

      <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

      <p>This subsection of the <a href="http://www.uniprot.org/manual/cross%5Freferences%5Fsection">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

      Seed defective Arabidopsis mutants

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      EU089963 mRNA Translation: ABW80569.1
      EU090805 mRNA Translation: ABU50828.1
      EU090805 mRNA Translation: ABU50829.1
      EF081156 mRNA Translation: ABN80998.1
      HQ857557 mRNA Translation: AEW48251.1
      HQ857558 mRNA Translation: AEW48252.1
      AB011482 Genomic DNA Translation: BAB08794.1 Sequence problems.
      AB011482 Genomic DNA Translation: BAB08795.1 Sequence problems.
      CP002688 Genomic DNA Translation: AED96924.1
      BT010433 mRNA Translation: AAQ62434.1
      AK175602 mRNA Translation: BAD43365.1
      RefSeqiNP_200567.2, NM_125140.4 [B0F481-1]

      3D structure databases

      Select the link destinations:

      Protein Data Bank Europe

      More...
      PDBei

      Protein Data Bank RCSB

      More...
      RCSB PDBi

      Protein Data Bank Japan

      More...
      PDBji
      Links Updated
      PDB entryMethodResolution (Å)ChainPositionsPDBsum
      4A0FX-ray2.71A/B23-833[»]
      4A0GX-ray2.50A/B/C/D23-833[»]
      4A0HX-ray2.81A/B23-833[»]
      4A0RX-ray2.68A/B23-833[»]
      SMRiB0F481
      ModBaseiSearch...
      PDBe-KBiSearch...

      Protein-protein interaction databases

      STRINGi3702.AT5G57590.1

      Proteomic databases

      PaxDbiB0F481
      PRIDEiB0F481
      ProteomicsDBi240795 [B0F481-1]

      Genome annotation databases

      EnsemblPlantsiAT5G57590.1; AT5G57590.1; AT5G57590 [B0F481-1]
      GeneIDi835863
      GrameneiAT5G57590.1; AT5G57590.1; AT5G57590 [B0F481-1]
      KEGGiath:AT5G57590

      Organism-specific databases

      AraportiAT5G57590
      TAIRilocus:2174532, AT5G57590

      Phylogenomic databases

      eggNOGiKOG1401, Eukaryota
      HOGENOMiCLU_010794_0_0_1
      InParanoidiB0F481
      KOiK19562
      OMAiKVEWYRG
      OrthoDBi289012at2759
      PhylomeDBiB0F481

      Enzyme and pathway databases

      UniPathwayiUPA00078;UER00160
      UPA00078;UER00161
      BioCyciMetaCyc:MONOMER-8566
      BRENDAi2.6.1.62, 399
      6.3.3.3, 399

      Miscellaneous databases

      Protein Ontology

      More...
      PROi
      PR:B0F481

      Gene expression databases

      ExpressionAtlasiB0F481, baseline and differential
      GenevisibleiB0F481, AT

      Family and domain databases

      Gene3Di3.40.640.10, 1 hit
      HAMAPiMF_00336, BioD, 1 hit
      InterProiView protein in InterPro
      IPR005814, Aminotrans_3
      IPR004472, DTB_synth_BioD
      IPR027417, P-loop_NTPase
      IPR015424, PyrdxlP-dep_Trfase
      IPR015421, PyrdxlP-dep_Trfase_major
      PfamiView protein in Pfam
      PF00202, Aminotran_3, 1 hit
      SUPFAMiSSF52540, SSF52540, 1 hit
      SSF53383, SSF53383, 1 hit
      PROSITEiView protein in PROSITE
      PS00600, AA_TRANSFER_CLASS_3, 1 hit

      ProtoNet; Automatic hierarchical classification of proteins

      More...
      ProtoNeti
      Search...

      MobiDB: a database of protein disorder and mobility annotations

      More...
      MobiDBi
      Search...

      <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

      <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBIODA_ARATH
      <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: B0F481
      Secondary accession number(s): B0F482
      , Q681L5, Q6NQL9, Q9FKL4, Q9FKL5
      <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2012
      Last sequence update: February 26, 2008
      Last modified: August 12, 2020
      This is version 94 of the entry and version 1 of the sequence. See complete history.
      <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programPlant Protein Annotation Program

      <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

      Keywords - Technical termi

      3D-structure, Reference proteome

      Documents

      1. Arabidopsis thaliana
        Arabidopsis thaliana: entries and gene names
      2. PATHWAY comments
        Index of metabolic and biosynthesis pathways
      3. PDB cross-references
        Index of Protein Data Bank (PDB) cross-references
      4. SIMILARITY comments
        Index of protein domains and families
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