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UniProtKB - B0EXJ8 (HTOMT_CATRO)
Protein
Tabersonine 16-O-methyltransferase
Gene
16OMT
Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Functioni
16-O-methyltransferase involved in the biosynthesis of vindoline. Highly specific for 16-hydroxytabersonine. No activity with tabersonine, 3-hydroxytyramine, 4-hydroxytyramine, 5-hydroxytryptamine (5HT), 2,3-dihydro-3-hydroxytabersonine, lochnericine, hoerhammericine, 16-hydroxy-2,3-dihydro-3-hydroxytabersonine, 16-hydroxylochnericine, 16-hydroxyhoerhammericine, quercetin, kaempferol and caffeic acid as substrates.
1 PublicationCatalytic activityi
- 16-hydroxytabersonine + S-adenosyl-L-methionine = 16-methoxytabersonine + H+ + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.941 Publication
Kineticsi
- KM=2.6 µM for 16-hydroxytabersonine1 Publication
- KM=21.7 µM for S-adenosyl-L-methionine1 Publication
- Vmax=0.58 mmol/sec/mg enzyme toward 16-hydroxytabersonine1 Publication
- Vmax=0.79 mmol/sec/mg enzyme toward S-adenosyl-L-methionine1 Publication
pH dependencei
Optimum pH is 7.0-7.5.1 Publication
: vindoline biosynthesis Pathwayi
This protein is involved in the pathway vindoline biosynthesis, which is part of Alkaloid biosynthesis.View all proteins of this organism that are known to be involved in the pathway vindoline biosynthesis and in Alkaloid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 222 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 256 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Active sitei | 260 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 11-O-demethyl-17-O-deacetylvindoline O-methyltransferase activity Source: UniProtKB
- O-methyltransferase activity Source: InterPro
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- alkaloid biosynthetic process Source: UniProtKB
- methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Alkaloid metabolism |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.94, 1211 |
UniPathwayi | UPA00365 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:16OMT1 Publication |
Organismi | Catharanthus roseus (Madagascar periwinkle) (Vinca rosea) |
Taxonomic identifieri | 4058 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Catharanthinae › Catharanthus |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000412068 | 1 – 355 | Tabersonine 16-O-methyltransferaseAdd BLAST | 355 |
Expressioni
Tissue specificityi
Expressed in leaves and flowers. Detected in stems and roots. In leaves, expressed in epidermal cells.3 Publications
Developmental stagei
Peak of expression in 8-day-old seedlings. Highest expression in the youngest leaf pair.1 Publication
Inductioni
Up-regulated by herbivory.1 Publication
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 198 – 201 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation | 4 | |
Regioni | 222 – 223 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation | 2 | |
Regioni | 242 – 243 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation | 2 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.Curated
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR012967, Plant_MeTrfase_dimerisation IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11746, PTHR11746, 1 hit |
Pfami | View protein in Pfam PF08100, Dimerisation, 1 hit PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
i Sequence
Sequence statusi: Complete.
B0EXJ8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVQSEEFRG AQAQIWSQSC SFITSASLKC AVKLGIPDTI DNHGKPITLS
60 70 80 90 100
ELTNALVPPV HPSKAPFIYR LMRVLAKNGF CSEEQLDGET EPLYSLTPSS
110 120 130 140 150
RILLKKEPLN LRGIVLTMAD PVQLKAWESL SDWYQNEDDS STAFETAHGK
160 170 180 190 200
NFWGYSSEHM EHAEFFNEAM ASDSQLISKL LIGEYKFLFE GLASLVDIGG
210 220 230 240 250
GTGTIAKAIA KNFPQLKCTV FDLPHVVANL ESKENVEFVA GDMFEKIPSA
260 270 280 290 300
NAIFLKWILH DWNDEDCVKI LKSCKKAIPA KGGKVIIIDM VMYSDKKDDH
310 320 330 340 350
LVKTQTSMDM AMLVNFAAKE RCEKEWAFLF KEAGFSDYKI YPKLDFTRSL
IEVYP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF444544 mRNA Translation: ABR20103.1 |
Genome annotation databases
KEGGi | ag:ABR20103 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF444544 mRNA Translation: ABR20103.1 |
3D structure databases
SMRi | B0EXJ8 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:ABR20103 |
Enzyme and pathway databases
UniPathwayi | UPA00365 |
BRENDAi | 2.1.1.94, 1211 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR012967, Plant_MeTrfase_dimerisation IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11746, PTHR11746, 1 hit |
Pfami | View protein in Pfam PF08100, Dimerisation, 1 hit PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HTOMT_CATRO | |
Accessioni | B0EXJ8Primary (citable) accession number: B0EXJ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 27, 2011 |
Last sequence update: | February 26, 2008 | |
Last modified: | February 23, 2022 | |
This is version 41 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families