UniProtKB - B0BUL7 (SYE2_RICRO)
Protein
Glutamate--tRNA ligase 2
Gene
gltX2
Organism
Rickettsia rickettsii (strain Iowa)
Status
Functioni
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation
Catalytic activityi
- EC:6.1.1.17UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 243 | ATPUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- glutamate-tRNA ligase activity Source: UniProtKB-UniRule
- tRNA binding Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- glutamyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | RRIC452659:G1G9G-1101-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)Alternative name(s): Glutamyl-tRNA synthetase 2UniRule annotation Short name: GluRS 2UniRule annotation |
Gene namesi | Name:gltX2UniRule annotation Ordered Locus Names:RrIowa_1141 |
Organismi | Rickettsia rickettsii (strain Iowa) |
Taxonomic identifieri | 452659 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000367761 | 1 – 513 | Glutamate--tRNA ligase 2Add BLAST | 513 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationProtein-protein interaction databases
STRINGi | 452659.RrIowa_1141 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 11 – 21 | 'HIGH' regionUniRule annotationAdd BLAST | 11 | |
Motifi | 240 – 244 | 'KMSKS' regionUniRule annotation | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation
Phylogenomic databases
HOGENOMi | HOG000252722 |
KOi | K01885 |
OMAi | WDEGPFF |
Family and domain databases
CDDi | cd00808 GluRS_core, 1 hit |
Gene3Di | 1.10.10.350, 1 hit 3.40.50.620, 1 hit |
HAMAPi | MF_00022 Glu_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR008925 aa-tRNA-synth_I_codon-bd IPR020751 aa-tRNA-synth_I_codon-bd_sub2 IPR004527 Glu-tRNA-ligase_bac/mito IPR000924 Glu/Gln-tRNA-synth IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom IPR033910 GluRS_core IPR005728 Rickett_RPE IPR014729 Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00749 tRNA-synt_1c, 1 hit |
PRINTSi | PR00987 TRNASYNTHGLU |
SUPFAMi | SSF48163 SSF48163, 1 hit |
TIGRFAMsi | TIGR00464 gltX_bact, 1 hit TIGR01045 RPE1, 1 hit |
i Sequence
Sequence statusi: Complete.
B0BUL7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNNNVITRFA PSPTGFLHIG SARTALFNYL FARHHNGKFL LRIEDTDKER
60 70 80 90 100
STKEAVEAIF SGLKWLGLDW NGEVIFQSKR NNLYKEAALK LLQNGKAYYC
110 120 130 140 150
FTRQEEIERQ RQQALENKQH FIFNSEWRDK DPSIYPTDIK PVIRLKTPRE
160 170 180 190 200
GSITIHDTLQ GEVVIENSHI DDMVLLRADG TATYMLAVVV DDHDMGITHI
210 220 230 240 250
IRGNDHLTNA ARQLAIYQAF GYAVPSMTHI PLIHGADGAK LSKRHGALGI
260 270 280 290 300
EAYKDMGYLP ESLCNYLLRL GWSHGDDEII SMTQAIDWFN LDSLGKSPAK
310 320 330 340 350
LDFAKMNSLN SHYLRMLDND SLTSKIVEIL EQNYNKLLQK LAYREEFGGN
360 370 380 390 400
TERSTAAYID IREDASTGLT YKLPLAVELP KKFKVSEQEI GYIKQAMPSL
410 420 430 440 450
LVRSETLLEL TRLAQIYLVD SPIIYSQDSK EIIENCDKNL IKQIIENLSE
460 470 480 490 500
LEQFDKESVQ NKFKEIAAAN DLKLNDIMKP VRALITGMTA SPSVFEIAEI
510
LGKENILKRL KII
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000766 Genomic DNA Translation: ABY72927.1 |
RefSeqi | WP_012151117.1, NC_010263.3 |
Genome annotation databases
EnsemblBacteriai | ABY72927; ABY72927; RrIowa_1141 |
KEGGi | rrj:RrIowa_1141 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000766 Genomic DNA Translation: ABY72927.1 |
RefSeqi | WP_012151117.1, NC_010263.3 |
3D structure databases
SMRi | B0BUL7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 452659.RrIowa_1141 |
Genome annotation databases
EnsemblBacteriai | ABY72927; ABY72927; RrIowa_1141 |
KEGGi | rrj:RrIowa_1141 |
Phylogenomic databases
HOGENOMi | HOG000252722 |
KOi | K01885 |
OMAi | WDEGPFF |
Enzyme and pathway databases
BioCyci | RRIC452659:G1G9G-1101-MONOMER |
Family and domain databases
CDDi | cd00808 GluRS_core, 1 hit |
Gene3Di | 1.10.10.350, 1 hit 3.40.50.620, 1 hit |
HAMAPi | MF_00022 Glu_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR008925 aa-tRNA-synth_I_codon-bd IPR020751 aa-tRNA-synth_I_codon-bd_sub2 IPR004527 Glu-tRNA-ligase_bac/mito IPR000924 Glu/Gln-tRNA-synth IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom IPR033910 GluRS_core IPR005728 Rickett_RPE IPR014729 Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00749 tRNA-synt_1c, 1 hit |
PRINTSi | PR00987 TRNASYNTHGLU |
SUPFAMi | SSF48163 SSF48163, 1 hit |
TIGRFAMsi | TIGR00464 gltX_bact, 1 hit TIGR01045 RPE1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYE2_RICRO | |
Accessioni | B0BUL7Primary (citable) accession number: B0BUL7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | February 26, 2008 | |
Last modified: | October 16, 2019 | |
This is version 73 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families