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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase family member 6

Gene

Enpp6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Choline-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin (By similarity). Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid (By similarity).By similarity

Catalytic activityi

A lysophosphatidylcholine + H2O = a monoacylglycerol + phosphocholine.
sn-glycero-3-phosphocholine + H2O = glycerol + phosphocholine.

Cofactori

Activity regulationi

Inhibited by EDTA and EGTA in vitro.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71NucleophileSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-RNO-6814848 Glycerophospholipid catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 (EC:3.1.4.-, EC:3.1.4.38)
Short name:
E-NPP 6
Short name:
NPP-6
Alternative name(s):
Choline-specific glycerophosphodiester phosphodiesterase
Glycerophosphocholine cholinephosphodiesterase
Short name:
GPC-Cpde
Gene namesi
Name:Enpp6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi1311645 Enpp6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000036692423 – 419Ectonucleotide pyrophosphatase/phosphodiesterase family member 6Add BLAST397
PropeptideiPRO_0000420894420 – 440Removed in mature formSequence analysisAdd BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71PhosphoserineCombined sources1
Glycosylationi100N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi118N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi142 ↔ 154By similarity
Glycosylationi341N-linked (GlcNAc...) asparagineCombined sources1
Glycosylationi404N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi412InterchainBy similarity
Lipidationi419GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiB0BND0
PRIDEiB0BND0

PTM databases

iPTMnetiB0BND0
PhosphoSitePlusiB0BND0
UniCarbKBiB0BND0

Expressioni

Gene expression databases

BgeeiENSRNOG00000009660 Expressed in 7 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiB0BND0 baseline and differential
GenevisibleiB0BND0 RN

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013005

Structurei

3D structure databases

SMRiB0BND0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2645 Eukaryota
COG1524 LUCA
GeneTreeiENSGT00760000119157
HOGENOMiHOG000112376
HOVERGENiHBG107851
InParanoidiB0BND0
OMAiGSWSRVM
OrthoDBiEOG091G06NA
PhylomeDBiB0BND0
TreeFamiTF330032

Family and domain databases

Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR029889 ENPP6
IPR002591 Phosphodiest/P_Trfase
PANTHERiPTHR10151:SF66 PTHR10151:SF66, 1 hit
PfamiView protein in Pfam
PF01663 Phosphodiest, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

B0BND0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGKLWTFLL LFGFSWVWPA SAHRKLLVLL LDGFRSDYIS EDALASLPGF
60 70 80 90 100
REIVNRGVKV DYLTPDFPSL SYPNYYTLMT GRHCEVHQMI GNYMWDPRTN
110 120 130 140 150
KSFDIGVNRD SLMPLWWNGS EPLWITLMKA RRKVYMYYWP GCEVEILGVR
160 170 180 190 200
PTYCLEYKNV PTDINFANAV SDALDSLKSG RADLAAIYHE RIDVEGHHYG
210 220 230 240 250
PSSPQRKDAL KAVDTVLKYM TQWIQERGLQ NDLNVILFSD HGMTDIFWMD
260 270 280 290 300
KVIELSKYIS LDDLQQVKDQ GPVVSLWPVP EKHSEIYHKL RTVEHMTVYE
310 320 330 340 350
KEAIPNRFYY KKGKFVSPLT LVADEGWFIA ESREALPFWM NSTGKREGWQ
360 370 380 390 400
HGWHGYDNEL MDMRGIFLAF GPDFKSNFRA APIRSVDVYN IMCNVVGITP
410 420 430 440
LPNNGSWSRV VCMLKSQTSS SPSIPPNSCA LVLILLLYFV
Length:440
Mass (Da):50,701
Last modified:February 26, 2008 - v1
Checksum:i4A76DB3E3EF8AD08
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3ZRI1D3ZRI1_RAT
Ectonucleotide pyrophosphatase/phos...
Enpp6 Enpp6_predicted, rCG_59003
362Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC158772 mRNA Translation: AAI58773.1
RefSeqiXP_006253218.1, XM_006253156.3
UniGeneiRn.8484

Genome annotation databases

EnsembliENSRNOT00000013005; ENSRNOP00000013005; ENSRNOG00000009660
GeneIDi306460

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC158772 mRNA Translation: AAI58773.1
RefSeqiXP_006253218.1, XM_006253156.3
UniGeneiRn.8484

3D structure databases

SMRiB0BND0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013005

PTM databases

iPTMnetiB0BND0
PhosphoSitePlusiB0BND0
UniCarbKBiB0BND0

Proteomic databases

PaxDbiB0BND0
PRIDEiB0BND0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013005; ENSRNOP00000013005; ENSRNOG00000009660
GeneIDi306460

Organism-specific databases

CTDi133121
RGDi1311645 Enpp6

Phylogenomic databases

eggNOGiKOG2645 Eukaryota
COG1524 LUCA
GeneTreeiENSGT00760000119157
HOGENOMiHOG000112376
HOVERGENiHBG107851
InParanoidiB0BND0
OMAiGSWSRVM
OrthoDBiEOG091G06NA
PhylomeDBiB0BND0
TreeFamiTF330032

Enzyme and pathway databases

ReactomeiR-RNO-6814848 Glycerophospholipid catabolism

Miscellaneous databases

PROiPR:B0BND0

Gene expression databases

BgeeiENSRNOG00000009660 Expressed in 7 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiB0BND0 baseline and differential
GenevisibleiB0BND0 RN

Family and domain databases

Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR029889 ENPP6
IPR002591 Phosphodiest/P_Trfase
PANTHERiPTHR10151:SF66 PTHR10151:SF66, 1 hit
PfamiView protein in Pfam
PF01663 Phosphodiest, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiENPP6_RAT
AccessioniPrimary (citable) accession number: B0BND0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 26, 2008
Last modified: November 7, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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