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Entry version 38 (11 Dec 2019)
Sequence version 1 (26 Feb 2008)
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Protein

Glutamate--methylamine ligase

Gene
N/A
Organism
Methylovorus mays
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the formation of N5-methyl-L-glutamine from glutamate and methylamine. In vitro, can also use ethylamine, hydroxylamine and ammonia, with 75%, 40% and 1% activity compared to methylamine, respectively.

2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Formation of theanine is repressed by a high concentration of glutamic acid.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.18 mM for methylamine1 Publication
  2. KM=0.57 mM for ethylamine1 Publication
  3. KM=3.9 mM for hydroxylamine1 Publication
  4. KM=84 mM for ammonia1 Publication
  5. KM=1.4 mM for glutamic acid (with methylamine as substrate)1 Publication
  6. KM=1.3 mM for glutamic acid (with ethylamine as substrate)1 Publication
  7. KM=1.3 mM for ATP (with methylamine as substrate)1 Publication
  8. KM=0.63 mM for ATP (with ethylamine as substrate)1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:MONOMER-17029

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Glutamate--methylamine ligaseCurated (EC:6.3.4.122 Publications)
Alternative name(s):
Gamma-glutamylmethylamide synthetase2 Publications
Short name:
GMAS2 Publications
Glutamate--ethylamine ligaseCurated (EC:6.3.1.62 Publications)
N(5)-ethyl-L-glutamine synthetaseCurated
Theanine synthetaseCurated
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMethylovorus mays
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri184077 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesMethylophilaceaeMethylovorus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the use of a specific protein in the biotechnological industry.<p><a href='/help/biotechnological_use' target='_top'>More...</a></p>Biotechnological usei

Could be used for theanine (N5-ethyl-L-glutamine) production, by coupling the reaction with the ATP-regeneration system of yeast sugar fermentation. Theanine occurs naturally in the tea plant (Camellia sinensis) and provides flavor to green tea. It has also several favorable physiological effects on mammals.2 Publications

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004318891 – 444Glutamate--methylamine ligaseAdd BLAST444

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

By methylamine.1 Publication

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
A9ZPH9

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.10.20.70, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR036651, Gln_synt_N
IPR014746, Gln_synth/guanido_kin_cat_dom
IPR008146, Gln_synth_cat_dom
IPR027303, Gln_synth_gly_rich_site
IPR017536, Glutamine_synthetase_typeIII

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00120, Gln-synt_C, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01230, Gln-synt_C, 1 hit

Superfamily database of structural and functional annotation

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SUPFAMi
SSF54368, SSF54368, 1 hit
SSF55931, SSF55931, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR03105, gln_synth_III, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00181, GLNA_ATP, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

A9ZPH9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKSLEEAQKF LEDHHVKYVL AQFVDIHGVA KVKSVPASHL NDILTTGAGF
60 70 80 90 100
AGGAIWGTGI APNGPDYMAI GELSTLSLIP WQPGYARLVC DGHVNGKPYE
110 120 130 140 150
FDTRVVLKQQ IARLAEKGWT LYTGLEPEFS LLKKDEHGAV HPFDDSDTLQ
160 170 180 190 200
KPCYDYKGIT RHSPFLEKLT ESLVEVGLDI YQIDHEDANG QFEINYTYAD
210 220 230 240 250
CLKSADDYIM FKMAASEIAN ELGIICSFMP KPFSNRPGNG MHMHMSIGDG
260 270 280 290 300
KKSLFQDDSD PSGLGLSKLA YHFLGGILAH APALAAVCAP TVNSYKRLVV
310 320 330 340 350
GRSLSGATWA PAYIAYGNNN RSTLVRIPYG RLELRLPDGS CNPYLATAAV
360 370 380 390 400
IAAGLDGVAR ELDPGTGRDD NLYDYSLEQL AEFGIGILPQ NLGEALDALE
410 420 430 440
ADQVIMDAMG PGLSKEFVEL KRMEWVDYMR HVSDWEINRY VQFY
Length:444
Mass (Da):48,925
Last modified:February 26, 2008 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF976581C78E7FCB6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti2K → T AA sequence (PubMed:17284842).Curated1
Sequence conflicti14H → D AA sequence (PubMed:17284842).Curated1
Sequence conflicti17K → V AA sequence (PubMed:17284842).Curated1
Sequence conflicti225 – 226IC → R AA sequence (PubMed:18175924).Curated2
Sequence conflicti236R → N AA sequence (PubMed:18175924).Curated1
Sequence conflicti239N → G AA sequence (PubMed:18175924).Curated1
Sequence conflicti312A → P AA sequence (PubMed:18175924).Curated1
Sequence conflicti314I → Y AA sequence (PubMed:18175924).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB333782 Genomic DNA Translation: BAF99006.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB333782 Genomic DNA Translation: BAF99006.1

3D structure databases

SMRiA9ZPH9
ModBaseiSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17029

Family and domain databases

Gene3Di3.10.20.70, 1 hit
InterProiView protein in InterPro
IPR036651, Gln_synt_N
IPR014746, Gln_synth/guanido_kin_cat_dom
IPR008146, Gln_synth_cat_dom
IPR027303, Gln_synth_gly_rich_site
IPR017536, Glutamine_synthetase_typeIII
PfamiView protein in Pfam
PF00120, Gln-synt_C, 1 hit
SMARTiView protein in SMART
SM01230, Gln-synt_C, 1 hit
SUPFAMiSSF54368, SSF54368, 1 hit
SSF55931, SSF55931, 1 hit
TIGRFAMsiTIGR03105, gln_synth_III, 1 hit
PROSITEiView protein in PROSITE
PS00181, GLNA_ATP, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGMAS_METMY
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A9ZPH9
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 4, 2015
Last sequence update: February 26, 2008
Last modified: December 11, 2019
This is version 38 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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