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Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 divalent ions per subunit.UniRule annotation
  • K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (metK)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22ATPUniRule annotation1
Metal bindingi24MagnesiumUniRule annotation1
Metal bindingi50PotassiumUniRule annotation1
Binding sitei63MethionineUniRule annotation1
Binding sitei107MethionineUniRule annotation1
Binding sitei257ATP; shared with neighboring subunitUniRule annotation1
Binding sitei257Methionine; shared with neighboring subunitUniRule annotation1
Binding sitei280ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation1
Binding sitei284ATP; shared with neighboring subunitUniRule annotation1
Binding sitei288MethionineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi182 – 184ATPUniRule annotation3
Nucleotide bindingi248 – 249ATPUniRule annotation2
Nucleotide bindingi263 – 264ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciCAUR324602:G1GAO-3072-MONOMER
UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotation
Ordered Locus Names:Caur_3020
OrganismiChloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
Taxonomic identifieri324602 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesChloroflexineaeChloroflexaceaeChloroflexus
Proteomesi
  • UP000002008 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000753671 – 403S-adenosylmethionine synthaseAdd BLAST403

Proteomic databases

PRIDEiA9WGQ3

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi324602.Caur_3020

Structurei

3D structure databases

ProteinModelPortaliA9WGQ3
SMRiA9WGQ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 117Flexible loopUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPH Bacteria
COG0192 LUCA
HOGENOMiHOG000245710
InParanoidiA9WGQ3
KOiK00789
OMAiPGHFLFT
OrthoDBiPOG091H00IO

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

A9WGQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANTFMNSPR FYFTSESVSE GHPDKMCDQI SDAILDAFLS HDPKARVAVE
60 70 80 90 100
TATTTGLIVV LGEVTYERGY IPIEEIVRRT VKEIGYTSAE YGFDADTCGV
110 120 130 140 150
MVAIHGQSPD IAMGVDKALE AKIGAMADDV EAVGAGDQGM MFGFACDETP
160 170 180 190 200
ELMPASIALA HRLIRRLERV RKDGTLPYLR PDAKSQVTVE YSFGKPVRVD
210 220 230 240 250
TVLISSQHAP DITQDQIRAD LIEHVIKTEI PEAWLDSQTK IFINPTGRFV
260 270 280 290 300
IGGPMGDSGL TGRKIIVDTY GGVARHGGGA FSGKDPSKVD RSAAYACRWV
310 320 330 340 350
AKNIVAAGLA RRVELQVSYA IGVARPLSLS VETFGTAAVP DEVILKAVNE
360 370 380 390 400
VFDLRPGAII RDLDLRRPIY RKTAAGGHFG RTDIDLPWER TNRVEELRRA

AGL
Length:403
Mass (Da):43,880
Last modified:February 5, 2008 - v1
Checksum:iC3BFDC834310BE7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000909 Genomic DNA Translation: ABY36219.1
RefSeqiWP_012258872.1, NC_010175.1
YP_001636608.1, NC_010175.1

Genome annotation databases

EnsemblBacteriaiABY36219; ABY36219; Caur_3020
GeneIDi5827494
KEGGicau:Caur_3020
PATRICifig|324602.8.peg.3421

Similar proteinsi

Entry informationi

Entry nameiMETK_CHLAA
AccessioniPrimary (citable) accession number: A9WGQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: February 28, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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