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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA), 6-phosphofructokinase (Caur_0869)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATP; via amide nitrogenUniRule annotation1
Metal bindingi116Magnesium; catalyticUniRule annotation1
Sitei117Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei140Proton acceptorUniRule annotation1
Binding sitei175Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei235SubstrateUniRule annotation1
Binding sitei272Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi78 – 79ATPUniRule annotation2
Nucleotide bindingi115 – 118ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCAUR324602:G1GAO-261-MONOMER
UniPathwayi
UPA00109;UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Caur_0258
OrganismiChloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
Taxonomic identifieri324602 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesChloroflexineaeChloroflexaceaeChloroflexus
Proteomesi
  • UP000002008 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000793081 – 356ATP-dependent 6-phosphofructokinaseAdd BLAST356

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi324602.Caur_0258

Structurei

3D structure databases

ProteinModelPortaliA9WCU2
SMRiA9WCU2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni138 – 140Substrate bindingUniRule annotation3
Regioni182 – 184Substrate bindingUniRule annotation3
Regioni278 – 281Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248869
InParanoidiA9WCU2
KOiK21071
OMAiRDGWRGP
OrthoDBiPOG091H01AC

Family and domain databases

HAMAPiMF_01976 Phosphofructokinase_III, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR012829 Phosphofructokinase_III
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02483 PFK_mixed, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

A9WCU2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASKKQRIGV LTSGGDAPGL NAVIRAVVKS ASGLGWEVIG IHDGFEGLLG
60 70 80 90 100
TKSYRVLTNA DVQGLLPRGG TILRTTNKGH FGPRRSDELS EADPYVRAVK
110 120 130 140 150
AIEEMGLRAL ITIGGEGTQR IALELHKLGA PVIGVPKTID NDLAGTDRTF
160 170 180 190 200
GFDTALQVAT DAIDRLHTTA ASHNRVMVLE VMGRHTGWIA LHAGLAGGAD
210 220 230 240 250
VILIPEIPFS IERVAEKVMA RDQQGSSFSI IVVAEGARPR GGSEMYIAEG
260 270 280 290 300
RLGGIGHWVG EQLEKLTAKE VRVVVLGHLQ RGGSPSPYDR LLSTRYGAAA
310 320 330 340 350
VQAAARGIYG EMVALRGQDI VTVPLAEACG HLNRVRPHSD LVLCARSLGI

AFGDEL
Length:356
Mass (Da):37,703
Last modified:February 5, 2008 - v1
Checksum:iC8B7B858A07B643C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000909 Genomic DNA Translation: ABY33511.1
RefSeqiWP_012256167.1, NC_010175.1
YP_001633900.1, NC_010175.1

Genome annotation databases

EnsemblBacteriaiABY33511; ABY33511; Caur_0258
GeneIDi5824865
KEGGicau:Caur_0258
PATRICifig|324602.8.peg.298

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000909 Genomic DNA Translation: ABY33511.1
RefSeqiWP_012256167.1, NC_010175.1
YP_001633900.1, NC_010175.1

3D structure databases

ProteinModelPortaliA9WCU2
SMRiA9WCU2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi324602.Caur_0258

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY33511; ABY33511; Caur_0258
GeneIDi5824865
KEGGicau:Caur_0258
PATRICifig|324602.8.peg.298

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248869
InParanoidiA9WCU2
KOiK21071
OMAiRDGWRGP
OrthoDBiPOG091H01AC

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

BioCyciCAUR324602:G1GAO-261-MONOMER

Family and domain databases

HAMAPiMF_01976 Phosphofructokinase_III, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR012829 Phosphofructokinase_III
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02483 PFK_mixed, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_CHLAA
AccessioniPrimary (citable) accession number: A9WCU2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: November 7, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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