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Protein

Thiamine thiazole synthase, chloroplastic

Gene

THI1

Organism
Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102Substrate; via amide nitrogenUniRule annotation1
Binding sitei130Substrate; via amide nitrogenUniRule annotation1
Binding sitei195Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei226SubstrateUniRule annotation1
Binding sitei241SubstrateUniRule annotation1
Binding sitei293Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthase, chloroplasticUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:THI1UniRule annotation
ORF Names:POPTR_011G134900v3Imported
OrganismiPopulus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)Imported
Taxonomic identifieri3694 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus
Proteomesi
  • UP000006729 Componenti: Chromosome 11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

ChloroplastUniRule annotation, Plastid

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2242,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-224 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3694.POPTR_0011s13820.1

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 123Substrate bindingUniRule annotation2
Regioni303 – 305Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2960 Eukaryota
COG1635 LUCA
HOGENOMiHOG000106048
KOiK03146
OMAiMTEFTDI
OrthoDBiEOG09360HDE

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

A9PEX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATMATTLTS LSTKSQKLSP FDTSSSFHGT PISKPTLRMQ PTKSSSSPNV
60 70 80 90 100
SISMSSPPYD LNAFKFEPIK ESIVSREMTR RYMMDMITHA DTDVVIVGAG
110 120 130 140 150
SAGLSCAYEL SKNPSVKIAI VEQSVSPGGG AWLGGQLFSA MVVRKPAHLF
160 170 180 190 200
LNELGIEYDE QEDYVVIKHA ALFTSTIMSK LLARPNVKLF NAVAAEDLIV
210 220 230 240 250
KEGRVGGVVT NWALVSMNHD TQSCMDPNVM EAKVVVSSCG HDGPFGATGV
260 270 280 290 300
KRLKSIGMID SVPGMKALDM NAAEDAIVKL TREVVPGMIV TGMEVAEIDG
310 320 330 340 350
SPRMGPTFGA MMISGQKAAH LALKSLGMPN ALDGTFVGGI HPELILAAVE

SAEIAET
Length:357
Mass (Da):37,883
Last modified:February 5, 2008 - v1
Checksum:i479D7510633B7CF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF146887 mRNA Translation: ABK94930.1
CM009300 Genomic DNA Translation: PNT13300.1
RefSeqiXP_002316981.2, XM_002316945.2
UniGeneiPth.4747

Genome annotation databases

EnsemblPlantsiPOPTR_0011s13820.1; POPTR_0011s13820.1; POPTR_0011s13820
GeneIDi7470933
GrameneiPOPTR_0011s13820.1; POPTR_0011s13820.1; POPTR_0011s13820
KEGGipop:7470933

Similar proteinsi

Entry informationi

Entry nameiA9PEX7_POPTR
AccessioniPrimary (citable) accession number: A9PEX7
Secondary accession number(s): B9I0A9
Entry historyiIntegrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: July 18, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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