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Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene

pfk2

Organism
Acholeplasma laidlawii (strain PG-8A)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Non-allosteric.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfk1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfk2)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15Diphosphate; via amide nitrogenUniRule annotation1
Metal bindingi115Magnesium; catalyticUniRule annotation1
Sitei116Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATPUniRule annotation1
Sitei142Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPiUniRule annotation1
Active sitei145Proton acceptorUniRule annotation1
Binding sitei245SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationImported, Transferase
Biological processGlycolysisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciALAI441768:ACL_RS05400-MONOMER
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotation (EC:2.7.1.90UniRule annotation)
Alternative name(s):
6-phosphofructokinase, pyrophosphate dependentUniRule annotation
PPi-dependent phosphofructokinaseUniRule annotation
Short name:
PPi-PFKUniRule annotation
Pyrophosphate-dependent 6-phosphofructose-1-kinaseUniRule annotation
Gene namesi
Name:pfk2Imported
Synonyms:pfpUniRule annotation
Ordered Locus Names:ACL_1092Imported
OrganismiAcholeplasma laidlawii (strain PG-8A)Imported
Taxonomic identifieri441768 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeAcholeplasma
Proteomesi
  • UP000008558 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi441768.ACL_1092

Structurei

3D structure databases

ProteinModelPortaliA9NH72
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 291PFKInterPro annotationAdd BLAST280

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni143 – 145Substrate bindingUniRule annotation3
Regioni189 – 191Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade "B2" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000007357
KOiK21071
OMAiAHDIGYF
OrthoDBiPOG091H01AC

Family and domain databases

HAMAPiMF_01978 Phosphofructokinase_II_B2, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
IPR011404 PPi-PFK_XF0274
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF036483 PFK_XF0274, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit

Sequencei

Sequence statusi: Complete.

A9NH72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLVGAALL GQSGGPTSVI NASAAGVFLE SLKHDEITEI YGAQHGIKGI
60 70 80 90 100
LDENFYDIRK EDVEELERLK YTPSSAIGSV RYKLKDYKKD PTDYNKLLEI
110 120 130 140 150
FKKYNIRYFF YNGGNDSMDT CLKISKFMKT AGWEMRVVGV PKTIDNDLAG
160 170 180 190 200
IDHSPGYGSA AKYVATSIME LYLDATVYNT QQFIIVEVMG RNAGWLTAAA
210 220 230 240 250
ALASVGGVKP DLMYLPETPF DMDKFYNQVG ELLKTGKSVF VVVSEGIKTA
260 270 280 290 300
EGKYIPEYET EIARDAFGHA NLGGTASVLA NHVGEKFGVK TRAIEFSLLQ
310 320 330 340 350
RSAAHIASQT DINEAFKVGQ IAVKKAINGT TNKFVGIQRV PGDKYKVKYP
360 370 380 390 400
LLPLKIVANA ERKVPLEWIL PDGQGLTQDF IDYALPLIQG ETKLEKVNGL
410
PRFAKLNKVL APKK
Length:414
Mass (Da):45,627
Last modified:February 5, 2008 - v1
Checksum:iDF38BC39BDB31718
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000896 Genomic DNA Translation: ABX81702.1
RefSeqiWP_012243033.1, NC_010163.1

Genome annotation databases

EnsemblBacteriaiABX81702; ABX81702; ACL_1092
KEGGiacl:ACL_1092

Similar proteinsi

Entry informationi

Entry nameiA9NH72_ACHLI
AccessioniPrimary (citable) accession number: A9NH72
Entry historyiIntegrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: June 20, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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