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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Acholeplasma laidlawii (strain PG-8A)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144Zinc 1UniRule annotation1
Metal bindingi147Zinc 1UniRule annotation1
Metal bindingi161Zinc 2UniRule annotation1
Metal bindingi164Zinc 2UniRule annotation1
Metal bindingi187Zinc 2UniRule annotation1
Metal bindingi190Zinc 2UniRule annotation1
Metal bindingi201Zinc 1UniRule annotation1
Metal bindingi204Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri131 – 213CR-typePROSITE-ProRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperoneUniRule annotationSAAS annotation
Biological processDNA replicationUniRule annotation, Stress responseUniRule annotation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotationImported
Ordered Locus Names:ACL_0551Imported
OrganismiAcholeplasma laidlawii (strain PG-8A)Imported
Taxonomic identifieri441768 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeAcholeplasma
Proteomesi
  • UP000008558 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi441768.ACL_0551

Structurei

3D structure databases

ProteinModelPortaliA9NFN9
SMRiA9NFN9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 69JInterPro annotationAdd BLAST65
Domaini131 – 213CR-typeInterPro annotationAdd BLAST83
Repeati144 – 151CXXCXGXG motifUniRule annotation8
Repeati161 – 168CXXCXGXG motifUniRule annotation8
Repeati187 – 194CXXCXGXG motifUniRule annotation8
Repeati201 – 208CXXCXGXG motifUniRule annotation8

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri131 – 213CR-typePROSITE-ProRule annotationAdd BLAST83

Keywords - Domaini

RepeatUniRule annotationSAAS annotation, Zinc-fingerUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105BZ5 Bacteria
COG0484 LUCA
HOGENOMiHOG000226717
KOiK03686
OMAiDMGGFAD
OrthoDBiPOG091H00PT

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd10719 DnaJ_zf, 1 hit
Gene3Di1.10.287.110, 1 hit
HAMAPiMF_01152 DnaJ, 1 hit
InterProiView protein in InterPro
IPR012724 DnaJ
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR001305 HSP_DnaJ_Cys-rich_dom
IPR036410 HSP_DnaJ_Cys-rich_dom_sf
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PF00684 DnaJ_CXXCXGXG, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 3 hits
SSF57938 SSF57938, 1 hit
TIGRFAMsiTIGR02349 DnaJ_bact, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
PS51188 ZF_CR, 1 hit

Sequencei

Sequence statusi: Complete.

A9NFN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRDYYDVL GISKSASQDE IKKAYRSLAK KYHPDVSKEK DAETKFKEVQ
60 70 80 90 100
EAYDVLNDSN KKAQYDRFGH AGTGQDPFGG AGGGFGGFGG FDDIISQFFG
110 120 130 140 150
GGQTRRTRQT SNVGEDLNMR MTIDFMEAVL GTTKNVSVDI TQDCGHCHGS
160 170 180 190 200
GAESSKDVHT CSKCHGQGFI NVDQRTMFGT MRSQQVCPQC QGEGQTIDNK
210 220 230 240 250
CHVCSGAGRV KAKKTVDVKV PAGVDNEMTL RVPGYGNGGR KGAESGDLYI
260 270 280 290 300
TFRVKPHKIF KRRGSDIILD VPITFTQAAL GDKIDIPTIY GEVELTIPAG
310 320 330 340 350
IQSGTELKLK NKGTKDPRTG KTGDQHVIVN IETPKNLSSE EKKLFEQLSK
360
LDSPRKKSGW DKFKSFFTN
Length:369
Mass (Da):40,433
Last modified:February 5, 2008 - v1
Checksum:iC7E5AD865E57BE2B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000896 Genomic DNA Translation: ABX81169.1
RefSeqiWP_012242500.1, NC_010163.1

Genome annotation databases

EnsemblBacteriaiABX81169; ABX81169; ACL_0551
KEGGiacl:ACL_0551

Similar proteinsi

Entry informationi

Entry nameiA9NFN9_ACHLI
AccessioniPrimary (citable) accession number: A9NFN9
Entry historyiIntegrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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