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UniProtKB - A9KT32 (NIGP_LACP7)

Entry version 66 (12 Aug 2020)
Sequence version 1 (05 Feb 2008)
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Protein

Nigerose phosphorylase

Gene

Cphy_1874

Organism
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reversible phosphorolysis of nigerose. Also shows a weak activity on kojibiose.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Does not require divalent metal ions.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 67 sec(-1) for nigerose. kcat is 110 sec(-1) for D-glucose.
  1. KM=1.7 mM for nigerose1 Publication
  2. KM=0.2 mM for phosphate1 Publication
  3. KM=3.3 mM for D-glucose1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 40 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei490Proton donorBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    Complete GO annotation on QuickGO ...

    GO - Biological processi

    Complete GO annotation on QuickGO ...

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionGlycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...    BioCyci    		CPHY357809:G1GAJ-1936-MONOMER

    BRENDA Comprehensive Enzyme Information System

    More...    BRENDAi    		2.4.1.279, 10424

    Protein family/group databases

    Carbohydrate-Active enZymes

    More...    CAZyi    		GH65, Glycoside Hydrolase Family 65

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Nigerose phosphorylase (EC:2.4.1.2791 Publication)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Ordered Locus Names:Cphy_1874
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiLachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri357809 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Firmicutes › Clostridia › Clostridiales › Lachnospiraceae ›
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000370 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    • Cytoplasm 1 Publication

    GO - Cellular componenti

    Complete GO annotation on QuickGO ...

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004187231 – 753Nigerose phosphorylaseAdd BLAST753

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer.

    1 Publication

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...    STRINGi    		357809.Cphy_1874

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...    SMRi    		A9KT32

    Database of comparative protein structure models

    More...    ModBasei    		Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni348 – 349Substrate bindingBy similarity2
    Regioni604 – 605Substrate bindingBy similarity2

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the glycosyl hydrolase 65 family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...    eggNOGi    		COG1554, Bacteria

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...    HOGENOMi    		CLU_006285_2_1_9

    KEGG Orthology (KO)

    More...    KOi    		K18783

    Identification of Orthologs from Complete Genome Data

    More...    OMAi    		ECAKFYY

    Database of Orthologous Groups

    More...    OrthoDBi    		179460at2

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...    Gene3Di    		1.50.10.10, 1 hit
    2.70.98.40, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...    InterProi    		View protein in InterPro
    IPR008928, 6-hairpin_glycosidase_sf
    IPR012341, 6hp_glycosidase-like_sf
    IPR011013, Gal_mutarotase_sf_dom
    IPR005194, Glyco_hydro_65_C
    IPR005195, Glyco_hydro_65_M
    IPR005196, Glyco_hydro_65_N
    IPR037018, Glyco_hydro_65_N_sf
    IPR017045, Malt_Pase/Glycosyl_Hdrlase

    Pfam protein domain database

    More...    Pfami    		View protein in Pfam
    PF03633, Glyco_hydro_65C, 1 hit
    PF03632, Glyco_hydro_65m, 1 hit
    PF03636, Glyco_hydro_65N, 1 hit

    PIRSF; a whole-protein classification database

    More...    PIRSFi    		PIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit

    Superfamily database of structural and functional annotation

    More...    SUPFAMi    		SSF48208, SSF48208, 1 hit
    SSF74650, SSF74650, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    A9KT32-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MNWTLTNSSL DKDSITSNGN RFLIGNGYLG IRGTLEEYRK EYFPAINLAG 
            60         70         80         90        100
    IYDQVGEGWR EPLNAPNALY TRIEVDEVEY QLPKIEPRYH ELSLDYRHGI 
           110        120        130        140        150
    LDRQTVWASN KGTIIVKSSR FASMKEKHLV VLNYSITADY DCEIIVYTGI 
           160        170        180        190        200
    DGSVWDIHGP HYDKVEFQKQ LLRHNETEIL NEKYLLESES KWNGHRLSIA 
           210        220        230        240        250
    AQTHENKDIV YVTEDIICNK EAKIELIESD KECLRKLTFH GKAKEEINFT 
           260        270        280        290        300
    KYITVFTSKD CVDYKEQSIK IVNHAKDTGY ERLQEEHKNV WEQLWNISEV 
           310        320        330        340        350
    TIEGDDEAND ALNYSLYHLH CIAPRHSKSL SIAARGLSGQ TYKGAVFWDT 
           360        370        380        390        400
    EMFMLDFFLY TQPEVAKTLL RYRIDTLEGA KKKAKLYGYE GAFYAWESQE 
           410        420        430        440        450
    GGYDACSDYN VTDVFTKRPM RTYFKDKQVH ISSAIVYGIR SYLNYTNDFS 
           460        470        480        490        500
    ILAEGGAETI LECAKFYYSL LEKKIGKEYY EIHDVIGPDE YHERVNNNAY 
           510        520        530        540        550
    TNRMAKLTFE TAIDILDHEK NKDEEFYIKL LKQYEIKDLL DKLKDACNKL 
           560        570        580        590        600
    YIPKPKDNSD LIEQFDGFFE LEDVSLEEVR SRLLHEKEYW GGAYGVASHT 
           610        620        630        640        650
    QVIKQADVVT MLVLFKEEYQ REVLQQNLNY YEPRTEHGSS LSACMYSLLY 
           660        670        680        690        700
    CMCDQPQYAY PFFMKSALAD WNGKGKEWAG LVYIGGTHPA AAGGAYMTAI 
           710        720        730        740        750
    KGFGGFQIEN GVIKATPRLP KHWVRLKYRV LYQGAIYEID ASKEQVSISK 

    IEM
    Length:753
    Mass (Da):87,173
    Last modified:February 5, 2008 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD8AF1D004AF8FCC1
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...    EMBLi

    GenBank nucleotide sequence database

    More...    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...    DDBJi
    Links Updated
    		CP000885 Genomic DNA Translation: ABX42243.1

    NCBI Reference Sequences

    More...    RefSeqi    		WP_012199897.1, NC_010001.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...    EnsemblBacteriai    		ABX42243; ABX42243; Cphy_1874

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...    KEGGi    		cpy:Cphy_1874

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		CP000885 Genomic DNA Translation: ABX42243.1
    RefSeqiWP_012199897.1, NC_010001.1

    3D structure databases

    SMRiA9KT32
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi357809.Cphy_1874

    Protein family/group databases

    CAZyiGH65, Glycoside Hydrolase Family 65

    Genome annotation databases

    EnsemblBacteriaiABX42243; ABX42243; Cphy_1874
    KEGGicpy:Cphy_1874

    Phylogenomic databases

    eggNOGiCOG1554, Bacteria
    HOGENOMiCLU_006285_2_1_9
    KOiK18783
    OMAiECAKFYY
    OrthoDBi179460at2

    Enzyme and pathway databases

    BioCyciCPHY357809:G1GAJ-1936-MONOMER
    BRENDAi2.4.1.279, 10424

    Family and domain databases

    Gene3Di1.50.10.10, 1 hit
    2.70.98.40, 1 hit
    InterProiView protein in InterPro
    IPR008928, 6-hairpin_glycosidase_sf
    IPR012341, 6hp_glycosidase-like_sf
    IPR011013, Gal_mutarotase_sf_dom
    IPR005194, Glyco_hydro_65_C
    IPR005195, Glyco_hydro_65_M
    IPR005196, Glyco_hydro_65_N
    IPR037018, Glyco_hydro_65_N_sf
    IPR017045, Malt_Pase/Glycosyl_Hdrlase
    PfamiView protein in Pfam
    PF03633, Glyco_hydro_65C, 1 hit
    PF03632, Glyco_hydro_65m, 1 hit
    PF03636, Glyco_hydro_65N, 1 hit
    PIRSFiPIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit
    SUPFAMiSSF48208, SSF48208, 1 hit
    SSF74650, SSF74650, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...    ProtoNeti    		Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...    MobiDBi    		Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNIGP_LACP7
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A9KT32
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: February 5, 2008
    Last modified: August 12, 2020
    This is version 66 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
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