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UniProtKB - A9KT32 (NIGP_LACP7)
Protein
Nigerose phosphorylase
Gene
Cphy_1874
Organism
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
Status
Functioni
Catalyzes the reversible phosphorolysis of nigerose. Also shows a weak activity on kojibiose.
1 PublicationCatalytic activityi
- EC:2.4.1.2791 Publication
Activity regulationi
Does not require divalent metal ions.1 Publication
Kineticsi
kcat is 67 sec(-1) for nigerose. kcat is 110 sec(-1) for D-glucose.
- KM=1.7 mM for nigerose1 Publication
- KM=0.2 mM for phosphate1 Publication
- KM=3.3 mM for D-glucose1 Publication
pH dependencei
Optimum pH is 7.0.1 Publication
Temperature dependencei
Optimum temperature is 40 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 490 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- 1,4-alpha-oligoglucan phosphorylase activity Source: CACAO
- carbohydrate binding Source: InterPro
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.1.279, 10424 |
Protein family/group databases
CAZyi | GH65, Glycoside Hydrolase Family 65 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:Cphy_1874 |
Organismi | Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans) |
Taxonomic identifieri | 357809 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Clostridia › Eubacteriales › Lachnospiraceae › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418723 | 1 – 753 | Nigerose phosphorylaseAdd BLAST | 753 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 357809.Cphy_1874 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 348 – 349 | Substrate bindingBy similarity | 2 | |
Regioni | 604 – 605 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 65 family.Curated
Phylogenomic databases
eggNOGi | COG1554, Bacteria |
HOGENOMi | CLU_006285_2_1_9 |
OMAi | ECAKFYY |
OrthoDBi | 179460at2 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit 2.70.98.40, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR011013, Gal_mutarotase_sf_dom IPR005194, Glyco_hydro_65_C IPR005195, Glyco_hydro_65_M IPR005196, Glyco_hydro_65_N IPR037018, Glyco_hydro_65_N_sf IPR017045, Malt_Pase/Glycosyl_Hdrlase |
Pfami | View protein in Pfam PF03633, Glyco_hydro_65C, 1 hit PF03632, Glyco_hydro_65m, 1 hit PF03636, Glyco_hydro_65N, 1 hit |
PIRSFi | PIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF74650, SSF74650, 1 hit |
i Sequence
Sequence statusi: Complete.
A9KT32-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNWTLTNSSL DKDSITSNGN RFLIGNGYLG IRGTLEEYRK EYFPAINLAG
60 70 80 90 100
IYDQVGEGWR EPLNAPNALY TRIEVDEVEY QLPKIEPRYH ELSLDYRHGI
110 120 130 140 150
LDRQTVWASN KGTIIVKSSR FASMKEKHLV VLNYSITADY DCEIIVYTGI
160 170 180 190 200
DGSVWDIHGP HYDKVEFQKQ LLRHNETEIL NEKYLLESES KWNGHRLSIA
210 220 230 240 250
AQTHENKDIV YVTEDIICNK EAKIELIESD KECLRKLTFH GKAKEEINFT
260 270 280 290 300
KYITVFTSKD CVDYKEQSIK IVNHAKDTGY ERLQEEHKNV WEQLWNISEV
310 320 330 340 350
TIEGDDEAND ALNYSLYHLH CIAPRHSKSL SIAARGLSGQ TYKGAVFWDT
360 370 380 390 400
EMFMLDFFLY TQPEVAKTLL RYRIDTLEGA KKKAKLYGYE GAFYAWESQE
410 420 430 440 450
GGYDACSDYN VTDVFTKRPM RTYFKDKQVH ISSAIVYGIR SYLNYTNDFS
460 470 480 490 500
ILAEGGAETI LECAKFYYSL LEKKIGKEYY EIHDVIGPDE YHERVNNNAY
510 520 530 540 550
TNRMAKLTFE TAIDILDHEK NKDEEFYIKL LKQYEIKDLL DKLKDACNKL
560 570 580 590 600
YIPKPKDNSD LIEQFDGFFE LEDVSLEEVR SRLLHEKEYW GGAYGVASHT
610 620 630 640 650
QVIKQADVVT MLVLFKEEYQ REVLQQNLNY YEPRTEHGSS LSACMYSLLY
660 670 680 690 700
CMCDQPQYAY PFFMKSALAD WNGKGKEWAG LVYIGGTHPA AAGGAYMTAI
710 720 730 740 750
KGFGGFQIEN GVIKATPRLP KHWVRLKYRV LYQGAIYEID ASKEQVSISK
IEM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000885 Genomic DNA Translation: ABX42243.1 |
RefSeqi | WP_012199897.1, NC_010001.1 |
Genome annotation databases
EnsemblBacteriai | ABX42243; ABX42243; Cphy_1874 |
KEGGi | cpy:Cphy_1874 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000885 Genomic DNA Translation: ABX42243.1 |
RefSeqi | WP_012199897.1, NC_010001.1 |
3D structure databases
SMRi | A9KT32 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 357809.Cphy_1874 |
Protein family/group databases
CAZyi | GH65, Glycoside Hydrolase Family 65 |
Genome annotation databases
EnsemblBacteriai | ABX42243; ABX42243; Cphy_1874 |
KEGGi | cpy:Cphy_1874 |
Phylogenomic databases
eggNOGi | COG1554, Bacteria |
HOGENOMi | CLU_006285_2_1_9 |
OMAi | ECAKFYY |
OrthoDBi | 179460at2 |
Enzyme and pathway databases
BRENDAi | 2.4.1.279, 10424 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit 2.70.98.40, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR011013, Gal_mutarotase_sf_dom IPR005194, Glyco_hydro_65_C IPR005195, Glyco_hydro_65_M IPR005196, Glyco_hydro_65_N IPR037018, Glyco_hydro_65_N_sf IPR017045, Malt_Pase/Glycosyl_Hdrlase |
Pfami | View protein in Pfam PF03633, Glyco_hydro_65C, 1 hit PF03632, Glyco_hydro_65m, 1 hit PF03636, Glyco_hydro_65N, 1 hit |
PIRSFi | PIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF74650, SSF74650, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NIGP_LACP7 | |
Accessioni | A9KT32Primary (citable) accession number: A9KT32 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | February 5, 2008 | |
Last modified: | February 23, 2022 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families