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UniProtKB - A9KT32 (NIGP_LACP7)

Entry version 69 (23 Feb 2022)
Sequence version 1 (05 Feb 2008)
Previous versions | rss
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Protein

Nigerose phosphorylase

Gene

Cphy_1874

Organism
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reversible phosphorolysis of nigerose. Also shows a weak activity on kojibiose.

1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Does not require divalent metal ions.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 67 sec(-1) for nigerose. kcat is 110 sec(-1) for D-glucose.
  1. KM=1.7 mM for nigerose1 Publication
  2. KM=0.2 mM for phosphate1 Publication
  3. KM=3.3 mM for D-glucose1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Temperature dependencei

Optimum temperature is 40 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei490Proton donorBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.4.1.279, 10424

Protein family/group databases

Carbohydrate-Active enZymes

More...CAZyi		GH65, Glycoside Hydrolase Family 65

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Nigerose phosphorylase (EC:2.4.1.2791 Publication)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Ordered Locus Names:Cphy_1874
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiLachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri357809 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Firmicutes › Clostridia › Eubacteriales › Lachnospiraceae ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000370 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004187231 – 753Nigerose phosphorylaseAdd BLAST753

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

1 Publication

Protein-protein interaction databases

STRING: functional protein association networks

More...STRINGi		357809.Cphy_1874

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		A9KT32

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni348 – 349Substrate bindingBy similarity2
Regioni604 – 605Substrate bindingBy similarity2

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the glycosyl hydrolase 65 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG1554, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_006285_2_1_9

Identification of Orthologs from Complete Genome Data

More...OMAi		ECAKFYY

Database of Orthologous Groups

More...OrthoDBi		179460at2

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.50.10.10, 1 hit
2.70.98.40, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR008928, 6-hairpin_glycosidase_sf
IPR012341, 6hp_glycosidase-like_sf
IPR011013, Gal_mutarotase_sf_dom
IPR005194, Glyco_hydro_65_C
IPR005195, Glyco_hydro_65_M
IPR005196, Glyco_hydro_65_N
IPR037018, Glyco_hydro_65_N_sf
IPR017045, Malt_Pase/Glycosyl_Hdrlase

Pfam protein domain database

More...Pfami		View protein in Pfam
PF03633, Glyco_hydro_65C, 1 hit
PF03632, Glyco_hydro_65m, 1 hit
PF03636, Glyco_hydro_65N, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF48208, SSF48208, 1 hit
SSF74650, SSF74650, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

A9KT32-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNWTLTNSSL DKDSITSNGN RFLIGNGYLG IRGTLEEYRK EYFPAINLAG 
        60         70         80         90        100
IYDQVGEGWR EPLNAPNALY TRIEVDEVEY QLPKIEPRYH ELSLDYRHGI 
       110        120        130        140        150
LDRQTVWASN KGTIIVKSSR FASMKEKHLV VLNYSITADY DCEIIVYTGI 
       160        170        180        190        200
DGSVWDIHGP HYDKVEFQKQ LLRHNETEIL NEKYLLESES KWNGHRLSIA 
       210        220        230        240        250
AQTHENKDIV YVTEDIICNK EAKIELIESD KECLRKLTFH GKAKEEINFT 
       260        270        280        290        300
KYITVFTSKD CVDYKEQSIK IVNHAKDTGY ERLQEEHKNV WEQLWNISEV 
       310        320        330        340        350
TIEGDDEAND ALNYSLYHLH CIAPRHSKSL SIAARGLSGQ TYKGAVFWDT 
       360        370        380        390        400
EMFMLDFFLY TQPEVAKTLL RYRIDTLEGA KKKAKLYGYE GAFYAWESQE 
       410        420        430        440        450
GGYDACSDYN VTDVFTKRPM RTYFKDKQVH ISSAIVYGIR SYLNYTNDFS 
       460        470        480        490        500
ILAEGGAETI LECAKFYYSL LEKKIGKEYY EIHDVIGPDE YHERVNNNAY 
       510        520        530        540        550
TNRMAKLTFE TAIDILDHEK NKDEEFYIKL LKQYEIKDLL DKLKDACNKL 
       560        570        580        590        600
YIPKPKDNSD LIEQFDGFFE LEDVSLEEVR SRLLHEKEYW GGAYGVASHT 
       610        620        630        640        650
QVIKQADVVT MLVLFKEEYQ REVLQQNLNY YEPRTEHGSS LSACMYSLLY 
       660        670        680        690        700
CMCDQPQYAY PFFMKSALAD WNGKGKEWAG LVYIGGTHPA AAGGAYMTAI 
       710        720        730        740        750
KGFGGFQIEN GVIKATPRLP KHWVRLKYRV LYQGAIYEID ASKEQVSISK 

IEM
Length:753
Mass (Da):87,173
Last modified:February 5, 2008 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD8AF1D004AF8FCC1
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
CP000885 Genomic DNA Translation: ABX42243.1

NCBI Reference Sequences

More...RefSeqi		WP_012199897.1, NC_010001.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		ABX42243; ABX42243; Cphy_1874

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		cpy:Cphy_1874

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000885 Genomic DNA Translation: ABX42243.1
RefSeqiWP_012199897.1, NC_010001.1

3D structure databases

SMRiA9KT32
ModBaseiSearch...

Protein-protein interaction databases

STRINGi357809.Cphy_1874

Protein family/group databases

CAZyiGH65, Glycoside Hydrolase Family 65

Genome annotation databases

EnsemblBacteriaiABX42243; ABX42243; Cphy_1874
KEGGicpy:Cphy_1874

Phylogenomic databases

eggNOGiCOG1554, Bacteria
HOGENOMiCLU_006285_2_1_9
OMAiECAKFYY
OrthoDBi179460at2

Enzyme and pathway databases

BRENDAi2.4.1.279, 10424

Family and domain databases

Gene3Di1.50.10.10, 1 hit
2.70.98.40, 1 hit
InterProiView protein in InterPro
IPR008928, 6-hairpin_glycosidase_sf
IPR012341, 6hp_glycosidase-like_sf
IPR011013, Gal_mutarotase_sf_dom
IPR005194, Glyco_hydro_65_C
IPR005195, Glyco_hydro_65_M
IPR005196, Glyco_hydro_65_N
IPR037018, Glyco_hydro_65_N_sf
IPR017045, Malt_Pase/Glycosyl_Hdrlase
PfamiView protein in Pfam
PF03633, Glyco_hydro_65C, 1 hit
PF03632, Glyco_hydro_65m, 1 hit
PF03636, Glyco_hydro_65N, 1 hit
PIRSFiPIRSF036289, Glycosyl_hydrolase_malt_phosph, 1 hit
SUPFAMiSSF48208, SSF48208, 1 hit
SSF74650, SSF74650, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNIGP_LACP7
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A9KT32
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: February 5, 2008
Last modified: February 23, 2022
This is version 69 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
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