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Protein

Hoefavidin

Gene

HPDFL43_17171

Organism
Hoeflea phototrophica (strain DSM 17068 / NCIMB 14078 / DFL-43)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The exact role played by hoefavidin in the host organism is still obscure. Forms a strong non-covalent complex with biotin and 2-iminobiotin.1 Publication

Temperature dependencei

Is hyperthermostable. Displays a Tm value of 84.9 degrees Celsius, which increases to 96.2 degrees Celsius upon biotin binding.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42Biotin1 Publication1
Binding sitei46Biotin1 Publication1
Binding sitei76Biotin; via amide nitrogen1 Publication1
Binding sitei148Biotin1 Publication1

GO - Molecular functioni

  • biotin binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Keywordsi

LigandBiotin

Names & Taxonomyi

Protein namesi
Recommended name:
Hoefavidin1 Publication
Gene namesi
ORF Names:HPDFL43_17171Imported
OrganismiHoeflea phototrophica (strain DSM 17068 / NCIMB 14078 / DFL-43)
Taxonomic identifieri411684 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeHoeflea
Proteomesi
  • UP000004291 Componentsi: Chromosome, Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

The specific properties of hoefavidin make it an attractive target for novel biotechnological applications, including drug delivery, nanotechnology, and molecular labeling.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000043458823 – 164HoefavidinAdd BLAST142

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi77 ↔ 1081 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer (PubMed:26126731). A later study shows that hoefavidin can exist in different homooligomeric states: dimer, tetramer, hexamer and octamer (PubMed:29720692). The X-ray structure of the intact hoefavidin reveals unique crystal packing generated by an octameric cylindrical structure wherein the C-terminal segments of each monomer are introduced into the entrance of the biotin-binding site of an adjacent non-canonical monomer (PubMed:26126731).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi411684.HPDFL43_17171

Structurei

Secondary structure

1164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliA9D857
SMRiA9D857
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 155Avidin-likePROSITE-ProRule annotationAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 70Biotin binding1 Publication3
Regioni110 – 112Biotin binding1 Publication3

Sequence similaritiesi

Belongs to the avidin/streptavidin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107377 Bacteria
ENOG410ZB3U LUCA
OrthoDBiPOG091H1MQT

Family and domain databases

Gene3Di2.40.128.30, 1 hit
InterProiView protein in InterPro
IPR036896 Avidin-like_sf
IPR005468 Avidin/str
PfamiView protein in Pfam
PF01382 Avidin, 1 hit
SUPFAMiSSF50876 SSF50876, 1 hit
PROSITEiView protein in PROSITE
PS51326 AVIDIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A9D857-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNKVLAIVLT ITVAGFAQTA FADDHAMSPD MKLLAGASNW VNQSGSVAQF
60 70 80 90 100
VFTPSPTQPQ TYEVSGNYIN NAQGTGCKGT PYPLSGAYYS GNQIISFSVV
110 120 130 140 150
WSNASANCQS ATGWTGYFDF SGSQAVLKTD WNLAFYSGST PAIQQGQDDF
160
MQSVATVSES LLTE
Length:164
Mass (Da):17,412
Last modified:February 5, 2008 - v1
Checksum:i66250B7CFF54B294
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABIA03000004 Genomic DNA Translation: EDQ33228.1
RefSeqiWP_007199185.1, NZ_CM002917.1

Genome annotation databases

EnsemblBacteriaiEDQ33228; EDQ33228; HPDFL43_17171

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABIA03000004 Genomic DNA Translation: EDQ33228.1
RefSeqiWP_007199185.1, NZ_CM002917.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Z27X-ray1.34A/B21-154[»]
4Z28X-ray1.66A/B21-154[»]
4Z2OX-ray1.17A21-154[»]
P153-164[»]
4Z2PX-ray1.60A/B21-154[»]
C/P153-164[»]
4Z2VX-ray1.39A/B21-154[»]
C/P153-164[»]
4Z6JX-ray2.40A/B21-164[»]
ProteinModelPortaliA9D857
SMRiA9D857
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi411684.HPDFL43_17171

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEDQ33228; EDQ33228; HPDFL43_17171

Phylogenomic databases

eggNOGiENOG4107377 Bacteria
ENOG410ZB3U LUCA
OrthoDBiPOG091H1MQT

Family and domain databases

Gene3Di2.40.128.30, 1 hit
InterProiView protein in InterPro
IPR036896 Avidin-like_sf
IPR005468 Avidin/str
PfamiView protein in Pfam
PF01382 Avidin, 1 hit
SUPFAMiSSF50876 SSF50876, 1 hit
PROSITEiView protein in PROSITE
PS51326 AVIDIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHOAVI_HOEPD
AccessioniPrimary (citable) accession number: A9D857
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 11, 2015
Last sequence update: February 5, 2008
Last modified: September 12, 2018
This is version 34 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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