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UniProtKB - A9CES4 (GDH_AGRFC)
Protein
Galactitol 2-dehydrogenase
Gene
Atu3164
Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Functioni
Involved in galactitol catabolism. Catalyzes the oxidation of galactitol to D-tagatose. Can also catalyze the oxidation of D-sorbitol to D-fructose.
1 PublicationCatalytic activityi
- EC:1.1.1.161 Publication
Kineticsi
kcat is 3.5 sec(-1) with galactitol as substrate. kcat is 4.4 sec(-1) with D-sorbitol as substrate.1 Publication
- KM=2.0 mM for galactitol1 Publication
- KM=1.9 mM for D-sorbitol1 Publication
: Carbohydrate metabolism Pathwayi
This protein is involved in Carbohydrate metabolism.1 PublicationView all proteins of this organism that are known to be involved in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 36 | NADBy similarity | 1 | |
Binding sitei | 86 | NAD; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 152 | Proton acceptorBy similarity | 1 | |
Binding sitei | 152 | NADBy similarity | 1 | |
Binding sitei | 156 | NADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 15 – 17 | NADBy similarity | 3 | |
Nucleotide bindingi | 59 – 60 | NADBy similarity | 2 |
GO - Molecular functioni
- galactitol 2-dehydrogenase activity Source: UniProtKB
GO - Biological processi
- galactitol metabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Carbohydrate metabolism |
Ligand | NAD |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:Atu3164Imported |
Organismi | Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) |
Taxonomic identifieri | 176299 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Hyphomicrobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Deletion mutant cannot grow on D-galactitol. Does not exhibit a growth defect when grown on D-altritol.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_5002736555 | 1 – 256 | Galactitol 2-dehydrogenaseSequence analysisAdd BLAST | 256 |
Expressioni
Inductioni
Up-regulated by growth on D-altritol or galactitol.1 Publication
Family & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Phylogenomic databases
eggNOGi | COG1028, Bacteria |
HOGENOMi | CLU_010194_1_0_5 |
OMAi | VGTLGKQ |
PhylomeDBi | A9CES4 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
A9CES4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLNNKVALI TGAARGIGLG FAQAFAAEGA KVIIADIDIA RATTSAAAIG
60 70 80 90 100
PAAKAVKLDV TDLAQIDAVV KAVDEEFGGI DILVNNAAIF DMAPINGITE
110 120 130 140 150
ESYERVFDIN LKGPMFMMKA VSNVMIARAR GGKIINMASQ AGRRGEALVT
160 170 180 190 200
LYCASKAAII SATQSAALAL VKHGINVNAI APGVVDGEHW EVVDAHFAKW
210 220 230 240 250
EGLKPGEKKA AVAKSVPIGR FATPDDIKGL AVFLASADSD YILAQTYNVD
GGNWMS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE007870 Genomic DNA Translation: AAK90222.2 |
RefSeqi | NP_357437.2, NC_003063.2 WP_010972809.1, NC_003063.2 |
Genome annotation databases
EnsemblBacteriai | AAK90222; AAK90222; Atu3164 |
GeneIDi | 66223482 |
KEGGi | atu:Atu3164 |
PATRICi | fig|176299.10.peg.3009 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE007870 Genomic DNA Translation: AAK90222.2 |
RefSeqi | NP_357437.2, NC_003063.2 WP_010972809.1, NC_003063.2 |
3D structure databases
SMRi | A9CES4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 176299.Atu3164 |
Genome annotation databases
EnsemblBacteriai | AAK90222; AAK90222; Atu3164 |
GeneIDi | 66223482 |
KEGGi | atu:Atu3164 |
PATRICi | fig|176299.10.peg.3009 |
Phylogenomic databases
eggNOGi | COG1028, Bacteria |
HOGENOMi | CLU_010194_1_0_5 |
OMAi | VGTLGKQ |
PhylomeDBi | A9CES4 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GDH_AGRFC | |
Accessioni | A9CES4Primary (citable) accession number: A9CES4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 10, 2019 |
Last sequence update: | January 15, 2008 | |
Last modified: | February 23, 2022 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families