UniProtKB - A9CEQ8 (GCI_AGRFC)
Protein
D-galactarolactone cycloisomerase
Gene
gci
Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Functioni
Catalyzes the ring opening of D-galactaro-1,4-lactone to yield 5-keto-4-deoxy-D-glucarate (KDG) via a beta-elimination reaction. This is a step in the oxidative degradation pathway of D-galacturonate, which allows A.tumefaciens to utilize D-galacturonate as a sole carbon source. To a lesser extent, can also use D-glucaro-1,4-lactone as substrate to produce KDG, but cannot use D-galactaro-1,5-lactone, D-glucaro-6,3-lactone and linear D-glucarate.2 Publications
Catalytic activityi
Cofactori
Mg2+1 Publication
pH dependencei
Optimum pH is 8.5.1 Publication
: D-galacturonate degradation via prokaryotic oxidative pathway Pathwayi
This protein is involved in the pathway D-galacturonate degradation via prokaryotic oxidative pathway, which is part of Carbohydrate acid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway D-galacturonate degradation via prokaryotic oxidative pathway and in Carbohydrate acid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 194 | Magnesium | 1 | |
Metal bindingi | 220 | Magnesium | 1 | |
Metal bindingi | 246 | Magnesium | 1 | |
Sitei | 269 | Increases basicity of active site HisBy similarity | 1 | |
Active sitei | 296 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- isomerase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cellular amino acid catabolic process Source: InterPro
Keywordsi
Molecular function | Isomerase |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | AGRO:ATU3139-MONOMER MetaCyc:MONOMER-18901 |
UniPathwayi | UPA01050 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:gci Ordered Locus Names:Atu3139 |
Organismi | Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) |
Taxonomic identifieri | 176299 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000429433 | 1 – 378 | D-galactarolactone cycloisomeraseAdd BLAST | 378 |
Interactioni
Subunit structurei
Homooctamer.
2 PublicationsProtein-protein interaction databases
STRINGi | 176299.Atu3139 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | A9CEQ8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the mandelate racemase/muconate lactonizing enzyme family.Curated
Phylogenomic databases
eggNOGi | COG4948, Bacteria |
HOGENOMi | CLU_030273_3_0_5 |
OMAi | DWDTRAY |
Family and domain databases
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR029065, Enolase_C-like IPR034618, GLI IPR018110, Mandel_Rmase/mucon_lact_enz_CS IPR013342, Mandelate_racemase_C IPR013341, Mandelate_racemase_N_dom |
Pfami | View protein in Pfam PF13378, MR_MLE_C, 1 hit PF02746, MR_MLE_N, 1 hit |
SFLDi | SFLDF00553, galactarolactone_cycloisomeras, 1 hit |
SMARTi | View protein in SMART SM00922, MR_MLE, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
PROSITEi | View protein in PROSITE PS00908, MR_MLE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
A9CEQ8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKITAVRTHL LEHRLDTPFE SASMRFDRRA HVLVEIECDD GTVGWGECLG
60 70 80 90 100
PARPNAAVVQ AYSGWLIGQD PRQTEKIWAV LYNALRDQGQ RGLSLTALSG
110 120 130 140 150
IDIALWDIKG KHYGASISML LGGRWRESVR AYATGSFKRD NVDRVSDNAS
160 170 180 190 200
EMAERRAEGF HACKIKIGFG VEEDLRVIAA VREAIGPDMR LMIDANHGYT
210 220 230 240 250
VTEAITLGDR AAGFGIDWFE EPVVPEQLDA YARVRAGQPI PVAGGETWHG
260 270 280 290 300
RYGMWQALSA GAVDILQPDL CGCGGFSEIQ KIATLATLHG VRIVPHVWGT
310 320 330 340 350
GVQIAAALQF MAAMTPDPVR VNPIEPIMEF DRTHNPFRQA VLREPLEAVN
360 370
GVVTIPDGPG LGIEINRDAL TEFRMPDP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE007870 Genomic DNA Translation: AAK90247.1 |
PIRi | AE2942 E98340 |
RefSeqi | NP_357462.1, NC_003063.2 WP_010972789.1, NC_003063.2 |
Genome annotation databases
EnsemblBacteriai | AAK90247; AAK90247; Atu3139 |
GeneIDi | 1134941 |
KEGGi | atu:Atu3139 |
PATRICi | fig|176299.10.peg.2984 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE007870 Genomic DNA Translation: AAK90247.1 |
PIRi | AE2942 E98340 |
RefSeqi | NP_357462.1, NC_003063.2 WP_010972789.1, NC_003063.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4GGB | X-ray | 2.00 | A | 1-378 | [»] | |
4HPN | X-ray | 1.60 | A | 1-378 | [»] | |
SMRi | A9CEQ8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 176299.Atu3139 |
Genome annotation databases
EnsemblBacteriai | AAK90247; AAK90247; Atu3139 |
GeneIDi | 1134941 |
KEGGi | atu:Atu3139 |
PATRICi | fig|176299.10.peg.2984 |
Phylogenomic databases
eggNOGi | COG4948, Bacteria |
HOGENOMi | CLU_030273_3_0_5 |
OMAi | DWDTRAY |
Enzyme and pathway databases
UniPathwayi | UPA01050 |
BioCyci | AGRO:ATU3139-MONOMER MetaCyc:MONOMER-18901 |
Family and domain databases
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR029065, Enolase_C-like IPR034618, GLI IPR018110, Mandel_Rmase/mucon_lact_enz_CS IPR013342, Mandelate_racemase_C IPR013341, Mandelate_racemase_N_dom |
Pfami | View protein in Pfam PF13378, MR_MLE_C, 1 hit PF02746, MR_MLE_N, 1 hit |
SFLDi | SFLDF00553, galactarolactone_cycloisomeras, 1 hit |
SMARTi | View protein in SMART SM00922, MR_MLE, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
PROSITEi | View protein in PROSITE PS00908, MR_MLE_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GCI_AGRFC | |
Accessioni | A9CEQ8Primary (citable) accession number: A9CEQ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 11, 2014 |
Last sequence update: | January 15, 2008 | |
Last modified: | December 2, 2020 | |
This is version 71 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families