UniProtKB - A9ABF9 (SYT_BURM1)
Protein
Threonine--tRNA ligase
Gene
thrS
Organism
Burkholderia multivorans (strain ATCC 17616 / 249)
Status
Functioni
Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).UniRule annotation
Catalytic activityi
- EC:6.1.1.3UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 333 | ZincUniRule annotation | 1 | |
Metal bindingi | 384 | Zinc; via tele nitrogenUniRule annotation | 1 | |
Metal bindingi | 510 | Zinc; via pros nitrogenUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
- threonine-tRNA ligase activity Source: UniProtKB-UniRule
- tRNA binding Source: UniProtKB-KW
GO - Biological processi
- threonyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)Alternative name(s): Threonyl-tRNA synthetaseUniRule annotation Short name: ThrRSUniRule annotation |
Gene namesi | Name:thrSUniRule annotation Ordered Locus Names:Bmul_1779, BMULJ_01461 |
Organismi | Burkholderia multivorans (strain ATCC 17616 / 249) |
Taxonomic identifieri | 395019 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000098551 | 1 – 635 | Threonine--tRNA ligaseAdd BLAST | 635 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 395019.Bmul_1779 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 61 | TGSPROSITE-ProRule annotationAdd BLAST | 61 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 242 – 533 | CatalyticUniRule annotationAdd BLAST | 292 |
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105C22 Bacteria COG0441 LUCA |
HOGENOMi | HOG000003878 |
KOi | K01868 |
OMAi | FYYDFAY |
Family and domain databases
CDDi | cd00771 ThrRS_core, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.40.50.800, 1 hit |
HAMAPi | MF_00184 Thr_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR002314 aa-tRNA-synt_IIb IPR006195 aa-tRNA-synth_II IPR004154 Anticodon-bd IPR036621 Anticodon-bd_dom_sf IPR012675 Beta-grasp_dom_sf IPR004095 TGS IPR012676 TGS-like IPR002320 Thr-tRNA-ligase_IIa IPR018163 Thr/Ala-tRNA-synth_IIc_edit IPR033728 ThrRS_core IPR012947 tRNA_SAD |
PANTHERi | PTHR11451 PTHR11451, 1 hit |
Pfami | View protein in Pfam PF03129 HGTP_anticodon, 1 hit PF02824 TGS, 1 hit PF00587 tRNA-synt_2b, 1 hit PF07973 tRNA_SAD, 1 hit |
PRINTSi | PR01047 TRNASYNTHTHR |
SMARTi | View protein in SMART SM00863 tRNA_SAD, 1 hit |
SUPFAMi | SSF55186 SSF55186, 1 hit SSF81271 SSF81271, 1 hit |
TIGRFAMsi | TIGR00418 thrS, 1 hit |
PROSITEi | View protein in PROSITE PS50862 AA_TRNA_LIGASE_II, 1 hit PS51880 TGS, 1 hit |
i Sequence
Sequence statusi: Complete.
A9ABF9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSIRLPDGS VRQYEHPVTV AEVAASIGPG LAKAALGGKL DGELVDTSAV
60 70 80 90 100
IDHDASLAIV TDKDPDGLDI IRHSTAHLLA YAVKELYPDA QVTIGPVIDN
110 120 130 140 150
GFYYDFAYHR PFTPEDLEKI EKRMQELAKK DEPVTRRVVS RDEAAGYFRS
160 170 180 190 200
IGEKYKAEII ESIPESDEIK LYSHGGFTDL CRGPHVPSTG KLKVFKLMKV
210 220 230 240 250
AGAYWRGDSK NEQLQRIYGT AWTKKEDQDQ YLHMLEEAEK RDHRKLGKQL
260 270 280 290 300
DLFHMQEESP GMVFWHPKGW ALWQQVEQYM RRRVNEAGYL EIKTPMIMDR
310 320 330 340 350
SLWEASGHWQ NYRENMFTTE SEKRDYAIKP MNCPGHVQVF KHGLRSYRDL
360 370 380 390 400
PLRYAEFGSC HRNEASGALH GLMRVRGFVQ DDAHIFCTEE QIIAESIAFN
410 420 430 440 450
KLAMSVYRDF GFDHIDIKLS LRPEQRMGSD ETWDRAEQGL REALTACGLQ
460 470 480 490 500
WEELPGEGAF YGPKIEYHIK DALGRSWQCG TLQLDMMLPE RLGAEYVAED
510 520 530 540 550
NSRRRPVMLH RAIVGSMERF LGILIEHHAG AMPVWLAPVQ AVVLNIAESQ
560 570 580 590 600
VEYAQSLAQT LQKQGLRVTA DLRNEKISYK IREHTLEKVP YLLVVGDKER
610 620 630
DAQTVAVRAR GGVDLGVMPV EAFVERLQED LRSFK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000868 Genomic DNA Translation: ABX15467.1 AP009385 Genomic DNA Translation: BAG43392.1 |
RefSeqi | WP_012213485.1, NC_010804.1 |
Genome annotation databases
EnsemblBacteriai | ABX15467; ABX15467; Bmul_1779 BAG43392; BAG43392; BMULJ_01461 |
KEGGi | bmj:BMULJ_01461 bmu:Bmul_1779 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000868 Genomic DNA Translation: ABX15467.1 AP009385 Genomic DNA Translation: BAG43392.1 |
RefSeqi | WP_012213485.1, NC_010804.1 |
3D structure databases
SMRi | A9ABF9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 395019.Bmul_1779 |
Genome annotation databases
EnsemblBacteriai | ABX15467; ABX15467; Bmul_1779 BAG43392; BAG43392; BMULJ_01461 |
KEGGi | bmj:BMULJ_01461 bmu:Bmul_1779 |
Phylogenomic databases
eggNOGi | ENOG4105C22 Bacteria COG0441 LUCA |
HOGENOMi | HOG000003878 |
KOi | K01868 |
OMAi | FYYDFAY |
Family and domain databases
CDDi | cd00771 ThrRS_core, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.40.50.800, 1 hit |
HAMAPi | MF_00184 Thr_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR002314 aa-tRNA-synt_IIb IPR006195 aa-tRNA-synth_II IPR004154 Anticodon-bd IPR036621 Anticodon-bd_dom_sf IPR012675 Beta-grasp_dom_sf IPR004095 TGS IPR012676 TGS-like IPR002320 Thr-tRNA-ligase_IIa IPR018163 Thr/Ala-tRNA-synth_IIc_edit IPR033728 ThrRS_core IPR012947 tRNA_SAD |
PANTHERi | PTHR11451 PTHR11451, 1 hit |
Pfami | View protein in Pfam PF03129 HGTP_anticodon, 1 hit PF02824 TGS, 1 hit PF00587 tRNA-synt_2b, 1 hit PF07973 tRNA_SAD, 1 hit |
PRINTSi | PR01047 TRNASYNTHTHR |
SMARTi | View protein in SMART SM00863 tRNA_SAD, 1 hit |
SUPFAMi | SSF55186 SSF55186, 1 hit SSF81271 SSF81271, 1 hit |
TIGRFAMsi | TIGR00418 thrS, 1 hit |
PROSITEi | View protein in PROSITE PS50862 AA_TRNA_LIGASE_II, 1 hit PS51880 TGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYT_BURM1 | |
Accessioni | A9ABF9Primary (citable) accession number: A9ABF9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | January 15, 2008 | |
Last modified: | May 8, 2019 | |
This is version 84 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families