UniProtKB - A8XJ44 (OXDA_CAEBR)
D-amino-acid oxidase
daao-1
Functioni
Catalyzes oxidative deamination of D-amino acids, in particular D-alanine, and could be responsible for the degradation of diet-derived D-alanine in the intestine. Acts on a variety of D-amino acids with greater preference towards those with basic and aromatic groups followed by those bearing neutral groups. Has no activity against acidic D-amino acids, L-amino acids or N-methyl-L-aspartic acid. May play a role in egg-laying events and early development.
By similarityCatalytic activityi
- EC:1.4.3.3By similarity
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 162 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 182 | FADBy similarity | 1 | |
Binding sitei | 220 | SubstrateBy similarity | 1 | |
Binding sitei | 275 | SubstrateBy similarity | 1 | |
Binding sitei | 299 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 303 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 4 – 18 | FADBy similarityAdd BLAST | 15 | |
Nucleotide bindingi | 34 – 35 | FADBy similarity | 2 | |
Nucleotide bindingi | 41 – 42 | FADBy similarity | 2 | |
Nucleotide bindingi | 46 – 48 | FADBy similarity | 3 | |
Nucleotide bindingi | 298 – 302 | FADBy similarity | 5 |
GO - Molecular functioni
- D-amino-acid oxidase activity Source: GO_Central
- FAD binding Source: InterPro
GO - Biological processi
- D-amino acid catabolic process Source: GO_Central
Keywordsi
Molecular function | Developmental protein, Oxidoreductase |
Ligand | FAD, Flavoprotein |
Names & Taxonomyi
Protein namesi | Recommended name: D-amino-acid oxidaseImported (EC:1.4.3.3By similarity)Short name: DAAOBy similarity Short name: DAMOXBy similarity Short name: DAOBy similarity |
Gene namesi | Name:daao-1Imported ORF Names:CBG13882Imported |
Organismi | Caenorhabditis briggsae |
Taxonomic identifieri | 6238 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | CBG13882a ; CBP09519 ; WBGene00034568 ; Cbr-daao-1 |
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Peroxisome
- peroxisome Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000317124 | 20 – 329 | D-amino-acid oxidaseSequence analysisAdd BLAST | 310 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 39 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 180 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 224 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PRIDEi | A8XJ44 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 6238.CBG13882 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 327 – 329 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3923, Eukaryota |
HOGENOMi | CLU_034311_0_2_1 |
InParanoidi | A8XJ44 |
OMAi | DEKCYPT |
OrthoDBi | 1363414at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR023209, DAO IPR006076, FAD-dep_OxRdtase |
PANTHERi | PTHR11530, PTHR11530, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit |
PIRSFi | PIRSF000189, D-aa_oxidase, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MPRICVLGAG IMGVSTALAI QERIPDSVVT IIAEKFSPNT TSDVAAGLIE
60 70 80 90 100
PYLCDDDVDR VISWTKSTIQ RIQEYMNEGN PGAESQEQSG YWLQSVKSVP
110 120 130 140 150
KWLEVMKNVK ILTGNELKMV AKRPEHKFGI FYTTWYLEPT PYIKWESDKF
160 170 180 190 200
LKNGGKIKNS KIQKIEDVEK EFGLFDVILN CTGIGARHLI GDNEVFPTRG
210 220 230 240 250
QILKVKCPSV KHFFIDDQFY ALLNDTTITL GGTADRHQWD RTINPKISEK
260 270 280 290 300
IFQENCKNIP SLRSAQVISS HVDLRPSRVT VRLEAEPDSK VIHNNGHGGS
310 320
GITLHWGCAL ECVELVKKVL AGKPGISKI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HE601467 Genomic DNA Translation: CAP32671.3 |
RefSeqi | XP_002634783.1, XM_002634737.1 |
Genome annotation databases
EnsemblMetazoai | CBG13882a.1; CBG13882a.1; WBGene00034568 |
GeneIDi | 8576775 |
KEGGi | cbr:CBG13882 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HE601467 Genomic DNA Translation: CAP32671.3 |
RefSeqi | XP_002634783.1, XM_002634737.1 |
3D structure databases
AlphaFoldDBi | A8XJ44 |
SMRi | A8XJ44 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 6238.CBG13882 |
Proteomic databases
PRIDEi | A8XJ44 |
Genome annotation databases
EnsemblMetazoai | CBG13882a.1; CBG13882a.1; WBGene00034568 |
GeneIDi | 8576775 |
KEGGi | cbr:CBG13882 |
Organism-specific databases
CTDi | 8576775 |
WormBasei | CBG13882a ; CBP09519 ; WBGene00034568 ; Cbr-daao-1 |
Phylogenomic databases
eggNOGi | KOG3923, Eukaryota |
HOGENOMi | CLU_034311_0_2_1 |
InParanoidi | A8XJ44 |
OMAi | DEKCYPT |
OrthoDBi | 1363414at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR023209, DAO IPR006076, FAD-dep_OxRdtase |
PANTHERi | PTHR11530, PTHR11530, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit |
PIRSFi | PIRSF000189, D-aa_oxidase, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | OXDA_CAEBR | |
Accessioni | A8XJ44Primary (citable) accession number: A8XJ44 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | January 15, 2008 | |
Last modified: | May 25, 2022 | |
This is version 86 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families