UniProtKB - A8X6H1 (EGL4_CAEBR)
cGMP-dependent protein kinase egl-4
egl-4
Functioni
Promotes chemoreceptor gene expression in response to increased cGMP levels by antagonizing the gene repression functions of the class II HDAC hda-4 and the mef-2 transcription factor. Regulates gene expression via recruitment of a histone deacetylase complex containing hda-2, saeg-1 and saeg-2. Represses body size and lifespan through the dbl-1 and insulin pathways, respectively. May also signal through daf-3 and/or daf-5. Role in egg-laying, dauer formation and motility. Regulates behavioral responses to various chemosensory stimuli in sensory neurons. Required for the initiation of long term adaptation to prolonged odor exposure which results in a decrease in odor seeking behavior. May regulate this process by phosphorylating tax-2, a subunit of cyclic nucleotide-gated channel tax-2/tax-4. In ASH sensory neurons, negatively regulates avoidance behavior to some bitter tastants, such as quinine, probably by phosphorylating rgs-2 and rgs-3 which are 2 regulator of G-protein signaling proteins. In AWB sensory neurons, involved in avoidance behavior to some repellent odors. In ASE left (ASEL) sensory neuron, involved in the sensing of environmental alkalinity downstream of receptor-type guanylate cyclase gcy-14. In sensory neurons, involved in the signaling pathway downstream of insulin, TGF-beta and receptor-type guanylate cyclase responsible for inducing quiescence after food intake. Might play a role in aversive olfactory learning in AWC neurons when an odor is associated with food deprivation, depending on the ins-1/age-1 signal from the AIA to the AWC neurons. Probably by regulating neuronal transmission downstream of lin-3 and receptor lin-23 and phospholipase plc-3 in ALA neurons, involved in the decrease in locomotion during the quiescent state that precedes each larval molt.
By similarityCatalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 349 | 3',5'-cGMP 2By similarity | 1 | |
Binding sitei | 403 | 3',5'-cGMP 2By similarity | 1 | |
Binding sitei | 468 | ATPPROSITE-ProRule annotationBy similarity | 1 | |
Active sitei | 562 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 234 – 237 | 3',5'-cGMP 1By similarity | 4 | |
Nucleotide bindingi | 244 – 245 | 3',5'-cGMP 1By similarity | 2 | |
Nucleotide bindingi | 358 – 361 | 3',5'-cGMP 2By similarity | 4 | |
Nucleotide bindingi | 368 – 369 | 3',5'-cGMP 2By similarity | 2 | |
Nucleotide bindingi | 444 – 452 | ATPPROSITE-ProRule annotationBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cGMP binding Source: UniProtKB-KW
- cGMP-dependent protein kinase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine kinase activity Source: RHEA
GO - Biological processi
- chemotaxis Source: UniProtKB-KW
Keywordsi
Molecular function | Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Chemotaxis |
Ligand | ATP-binding, cGMP, cGMP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: cGMP-dependent protein kinase egl-4By similarity (EC:2.7.11.12By similarity)Alternative name(s): Egg-laying defective protein 4By similarity |
Gene namesi | Name:egl-4Imported ORF Names:CBG08401Imported |
Organismi | Caenorhabditis briggsae |
Taxonomic identifieri | 6238 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | CBG08401a ; CBP30576 ; WBGene00030199 ; Cbr-egl-4 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: In resting AWC sensory neurons, localizes in cytoplasm. Prolonged exposure to attractive odorants sensed by AWC neurons results in nuclear translocation. Nuclear translocation is required for the adaptation to prolonged odor exposure and is controlled by G(o)-alpha subunit protein goa-1. Localization is regulated by cGMP levels: high cGMP levels result in cytoplasmic localization whereas low cGMP levels result in nuclear localization. Nuclear localization in AWC neurons is dependent on age-1. In addition, an intact sensory cilia structure is required for cytoplasmic localization in resting AWC neurons. In resting AWB sensory neurons, constitutive nuclear localization is dependent on goa-1. In resting ASH sensory neurons, localizes in both cytoplasm and nucleus. Cytoplasmic localization is important for negative regulation of quinine sensitivity in ASH neurons.By similarity
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000390490 | 1 – 749 | cGMP-dependent protein kinase egl-4Add BLAST | 749 |
Post-translational modificationi
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 438 – 698 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 261 | |
Domaini | 699 – 749 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 51 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 87 – 111 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 723 – 749 | DisorderedSequence analysisAdd BLAST | 27 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 30 – 96 | Sequence analysisAdd BLAST | 67 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 461 – 473 | Nuclear localization signalSequence analysisAdd BLAST | 13 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG0614, Eukaryota |
HOGENOMi | CLU_000288_73_2_1 |
InParanoidi | A8X6H1 |
OMAi | WIVKLYK |
OrthoDBi | 401933at2759 |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits cd05572, STKc_cGK, 1 hit |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR002374, cGMP_dep_kinase IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR014710, RmlC-like_jellyroll IPR008271, Ser/Thr_kinase_AS IPR035014, STKc_cGK |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF000559, cGMP-dep_kinase, 1 hit |
PRINTSi | PR00104, CGMPKINASE |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00888, CNMP_BINDING_1, 1 hit PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MYGSSRHHLD SFSSNDGNAF LVQVGSRTFE AHELQKLIPQ LEEAISRKDA
60 70 80 90 100
QLRQQQSIVE GHIKRISELE GEVTTLQREC DKLRSVLEQK AQSAASPGQP
110 120 130 140 150
PSPSPRTDQL GNDLQQKAVF PADGTQQRAK KIAVSAEPTN FENKPATLQH
160 170 180 190 200
YNKTVAAKQM IRDAVQKNDF LKQLAKEQII ELVNCMYEMR ARAGQWVIQE
210 220 230 240 250
GEPGDRLFVV AEGELQVSRE GATLGKMRAG TVMGELAILY NCTRTASVQA
260 270 280 290 300
LTDVQLWVLD RSVFQMITQR LGMERHSQII NFLSKVSIFA NLTEDRISKI
310 320 330 340 350
ADVMDQDYYD GGHYILRQGE KGDAFFVINS GQVKVTQQIE GEKEAREIRI
360 370 380 390 400
LNQGDFFGER ALLGDEVRTA NIIAQAPGVE VLTLDRESFT KLIGDLDTLR
410 420 430 440 450
KDYGDKERVA TLVREPPSPV KIVDDFREEF ANVTLKNVKR LATLGVGGFG
460 470 480 490 500
RVELVCVNGD KSKTYALKAL KKKHIVDTRQ QEHIFAERNI MMETSTDWIV
510 520 530 540 550
KLYKTFRDQK FVYMLLEVCL GGELWTTLRD RGHFDDYTAR FYVACVLEGL
560 570 580 590 600
EYLHRKNIVY RDLKPENCLL ANSGYLKLVD FGFAKKLASG RKTWTFCGTP
610 620 630 640 650
EYVSPEIILN KGHDQAADYW ALGIYICELM LGRPPFQASD PMKTYTLILK
660 670 680 690 700
GVDALEIPNR RIGKTATALV KKLCRDNPGE RLGSGSGGVN DIRKHRWFMG
710 720 730 740
FDWEGLRTKT LKPPILPKVN NPADVTNFDN YPPDNDVPPD EFSGWDEGF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HE601100 Genomic DNA Translation: CAP28232.2 |
Genome annotation databases
EnsemblMetazoai | CBG08401a.1; CBG08401a.1; WBGene00030199 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HE601100 Genomic DNA Translation: CAP28232.2 |
3D structure databases
AlphaFoldDBi | A8X6H1 |
SMRi | A8X6H1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 6238.CBG08401 |
Genome annotation databases
EnsemblMetazoai | CBG08401a.1; CBG08401a.1; WBGene00030199 |
Organism-specific databases
WormBasei | CBG08401a ; CBP30576 ; WBGene00030199 ; Cbr-egl-4 |
Phylogenomic databases
eggNOGi | KOG0614, Eukaryota |
HOGENOMi | CLU_000288_73_2_1 |
InParanoidi | A8X6H1 |
OMAi | WIVKLYK |
OrthoDBi | 401933at2759 |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits cd05572, STKc_cGK, 1 hit |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR002374, cGMP_dep_kinase IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR014710, RmlC-like_jellyroll IPR008271, Ser/Thr_kinase_AS IPR035014, STKc_cGK |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF000559, cGMP-dep_kinase, 1 hit |
PRINTSi | PR00104, CGMPKINASE |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00888, CNMP_BINDING_1, 1 hit PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EGL4_CAEBR | |
Accessioni | A8X6H1Primary (citable) accession number: A8X6H1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 2009 |
Last sequence update: | December 16, 2008 | |
Last modified: | May 25, 2022 | |
This is version 85 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families