UniProtKB - A8WTA7 (TRMB_CAEBR)
Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
CBG02883
Organism
Caenorhabditis briggsae
Status
Functioni
Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation
Catalytic activityi
- guanosine46 in tRNA + S-adenosyl-L-methionine = N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.33UniRule annotation
: N(7)-methylguanine-tRNA biosynthesis Pathwayi
This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotationView all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 74 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 152 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 155 | UniRule annotation | 1 |
GO - Molecular functioni
- tRNA (guanine-N7-)-methyltransferase activity Source: GO_Central
- tRNA binding Source: UniProtKB-UniRule
GO - Biological processi
- RNA (guanine-N7)-methylation Source: GO_Central
- tRNA methylation Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
UniPathwayi | UPA00989 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)Alternative name(s): tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation tRNA(m7G46)-methyltransferaseUniRule annotation |
Gene namesi | ORF Names:CBG02883 |
Organismi | Caenorhabditis briggsae |
Taxonomic identifieri | 6238 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | CBG02883 ; CBP00870 ; WBGene00025851 |
Subcellular locationi
Nucleus
- Nucleus UniRule annotation
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- tRNA methyltransferase complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000370563 | 1 – 256 | tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST | 256 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 97 – 98 | S-adenosyl-L-methionine bindingUniRule annotation | 2 | |
Regioni | 132 – 133 | S-adenosyl-L-methionine bindingUniRule annotation | 2 | |
Regioni | 230 – 232 | S-adenosyl-L-methionine bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3115, Eukaryota |
HOGENOMi | CLU_050910_3_1_1 |
InParanoidi | A8WTA7 |
OMAi | KWRIINQ |
OrthoDBi | 1417652at2759 |
Family and domain databases
HAMAPi | MF_03055, tRNA_methyltr_TrmB_euk, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR025763, Trm8_euk IPR003358, tRNA_(Gua-N-7)_MeTrfase_Trmb |
PANTHERi | PTHR23417, PTHR23417, 1 hit PTHR23417:SF16, PTHR23417:SF16, 1 hit |
Pfami | View protein in Pfam PF02390, Methyltransf_4, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00091, TIGR00091, 1 hit |
PROSITEi | View protein in PROSITE PS51625, SAM_MT_TRMB, 1 hit |
i Sequence
Sequence statusi: Complete.
A8WTA7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVDPVNSEM ELDNKPTCET VPGLPQKKHY RQRAHSNPHS DHDIEYPLTP
60 70 80 90 100
DHMDWSKQYG DYAAGRQVEF ADIGCGYGGL LMRLSPMFPE TLMIGMEIRV
110 120 130 140 150
KVSDYVNEKI QALRKHHEGA GHYRNIAVLR SNAMKYMPNY FRKGQLSKMF
160 170 180 190 200
FLFPDPHFKN KKHKWRIITP TLVSEYAYVL REQGLIYTIT DVKDLHDWMV
210 220 230 240 250
KHLNEHPLFE RLSDDEMKAD PVVAMLYEST EEGQKVTRND GEKWPAVFRR
LPNPPL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HE601438 Genomic DNA Translation: CAP23718.1 |
RefSeqi | XP_002631108.1, XM_002631062.1 |
Genome annotation databases
EnsemblMetazoai | CBG02883.1; CBG02883.1; WBGene00025851 |
GeneIDi | 8572622 |
KEGGi | cbr:CBG02883 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HE601438 Genomic DNA Translation: CAP23718.1 |
RefSeqi | XP_002631108.1, XM_002631062.1 |
3D structure databases
SMRi | A8WTA7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 6238.CBG02883 |
Genome annotation databases
EnsemblMetazoai | CBG02883.1; CBG02883.1; WBGene00025851 |
GeneIDi | 8572622 |
KEGGi | cbr:CBG02883 |
Organism-specific databases
CTDi | 8572622 |
WormBasei | CBG02883 ; CBP00870 ; WBGene00025851 |
Phylogenomic databases
eggNOGi | KOG3115, Eukaryota |
HOGENOMi | CLU_050910_3_1_1 |
InParanoidi | A8WTA7 |
OMAi | KWRIINQ |
OrthoDBi | 1417652at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00989 |
Family and domain databases
HAMAPi | MF_03055, tRNA_methyltr_TrmB_euk, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR025763, Trm8_euk IPR003358, tRNA_(Gua-N-7)_MeTrfase_Trmb |
PANTHERi | PTHR23417, PTHR23417, 1 hit PTHR23417:SF16, PTHR23417:SF16, 1 hit |
Pfami | View protein in Pfam PF02390, Methyltransf_4, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00091, TIGR00091, 1 hit |
PROSITEi | View protein in PROSITE PS51625, SAM_MT_TRMB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRMB_CAEBR | |
Accessioni | A8WTA7Primary (citable) accession number: A8WTA7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | January 15, 2008 | |
Last modified: | December 2, 2020 | |
This is version 76 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families