UniProtKB - A8R3S4 (QEDH_PSEPU)
Quinoprotein ethanol dehydrogenase
qedA
Functioni
Catalyzes the oxidation of ethanol and other primary alcohols to the corresponding aldehydes, except methanol, which is not a substrate (PubMed:7730276).
Uses a specific inducible cytochrome c550, encoded by the adjacent gene in the locus, as electron acceptor (By similarity).
Is a key enzyme of the carbon and energy metabolism during growth of P.putida on ethanol as the sole carbon and energy source (PubMed:18218017).
Displays lower activity on secondary alcohols, aldehydes and diols. Is not active with sugar alcohols such as glycerol and D-sorbitol (PubMed:7730276).
In vitro, reacts well with phenazine methosulfate (PMS) as an electron acceptor but not with NAD(P), potassium ferricyanide, or molecular oxygen (PubMed:7730276).
By similarity2 PublicationsCatalytic activityi
- a primary alcohol + 2 Fe(III)-[cytochrome c] = an aldehyde + 2 Fe(II)-[cytochrome c] + 2 H+2 PublicationsEC:1.1.2.82 PublicationsThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Cofactori
Protein has several cofactor binding sites:- pyrroloquinoline quinone1 PublicationNote: Binds 1 PQQ group non-covalently per subunit (PubMed:7730276). PQQ is inserted between disulfide Cys-139-Cys-140 and the plane of Trp-282 (By similarity).By similarity1 Publication
- Ca2+By similarityNote: Binds 2 calcium ions per subunit. One is located in the active-site cavity near PQQ and the second calcium binds at the N-terminus and contributes to the stability of the native enzyme.By similarity
Activity regulationi
Kineticsi
- KM=0.163 mM for ethanol1 Publication
- KM=1.62 mM for butan-1-ol1 Publication
- KM=24.9 mM for propane-1,2-diol1 Publication
- Vmax=26.3 µmol/min/mg enzyme with ethanol as substrate1 Publication
- Vmax=21.5 µmol/min/mg enzyme with butan-1-ol as substrate1 Publication
- Vmax=12.3 µmol/min/mg enzyme with propane-1,2-diol as substrate1 Publication
pH dependencei
: ethanol degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes acetate from ethanol. This subpathway is part of the pathway ethanol degradation, which is itself part of Alcohol metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetate from ethanol, the pathway ethanol degradation and in Alcohol metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 45 | Calcium 1By similarity | 1 | |
Metal bindingi | 51 | Calcium 1By similarity | 1 | |
Binding sitei | 95 | Pyrroloquinoline quinoneBy similarity | 1 | |
Binding sitei | 145 | Pyrroloquinoline quinoneBy similarity | 1 | |
Binding sitei | 189 | Pyrroloquinoline quinoneBy similarity | 1 | |
Metal bindingi | 213 | Calcium 2; catalyticBy similarity | 1 | |
Metal bindingi | 300 | Calcium 2; catalyticBy similarity | 1 | |
Active sitei | 350 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 350 | Calcium 2; catalyticBy similarity | 1 | |
Binding sitei | 378 | Pyrroloquinoline quinoneBy similarity | 1 | |
Binding sitei | 523 | Pyrroloquinoline quinoneBy similarity | 1 | |
Binding sitei | 587 | Pyrroloquinoline quinone; via amide nitrogenBy similarity | 1 |
GO - Molecular functioni
- alcohol dehydrogenase (cytochrome c) activity Source: UniProtKB-EC
- calcium ion binding Source: InterPro
GO - Biological processi
- ethanol catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Calcium, Metal-binding, PQQ |
Enzyme and pathway databases
UniPathwayi | UPA00780;UER00767 |
Names & Taxonomyi
Protein namesi | Recommended name: Quinoprotein ethanol dehydrogenase1 Publication (EC:1.1.2.82 Publications)Short name: QEDH1 Publication Alternative name(s): Quinoprotein alcohol dehydrogenase (cytochrome c)Curated Quinoprotein alcohol dehydrogenase (cytochrome c550)Curated Quinoprotein alcohol dehydrogenase ADH I1 Publication Short name: ADH I1 Publication |
Gene namesi | Name:qedA1 Publication |
Organismi | Pseudomonas putida (Arthrobacter siderocapsulatus) |
Taxonomic identifieri | 303 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Subcellular locationi
Other locations
- Periplasm 1 Publication
Other locations
- membrane Source: InterPro
- outer membrane-bounded periplasmic space Source: InterPro
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 34 | Sequence analysisAdd BLAST | 34 | |
ChainiPRO_0000419533 | 35 – 623 | Quinoprotein ethanol dehydrogenase1 PublicationAdd BLAST | 589 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 139 ↔ 140 | By similarity |
Keywords - PTMi
Disulfide bondExpressioni
Inductioni
Interactioni
Subunit structurei
Homodimer.
1 PublicationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 515 – 556 | WD 1Sequence analysisAdd BLAST | 42 | |
Repeati | 559 – 601 | WD 2Sequence analysisAdd BLAST | 43 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 207 – 209 | Pyrroloquinoline quinone bindingBy similarity | 3 | |
Regioni | 242 – 279 | DisorderedSequence analysisAdd BLAST | 38 | |
Regioni | 414 – 436 | DisorderedSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Signal, WD repeatFamily and domain databases
CDDi | cd10277, PQQ_ADH_I, 1 hit |
InterProi | View protein in InterPro IPR034119, ADHI IPR018391, PQQ_beta_propeller_repeat IPR017512, PQQ_MeOH/EtOH_DH IPR002372, PQQ_repeat IPR011047, Quinoprotein_ADH-like_supfam IPR001479, Quinoprotein_DH_CS |
Pfami | View protein in Pfam PF01011, PQQ, 1 hit PF13360, PQQ_2, 1 hit |
SMARTi | View protein in SMART SM00564, PQQ, 5 hits |
SUPFAMi | SSF50998, SSF50998, 1 hit |
TIGRFAMsi | TIGR03075, PQQ_enz_alc_DH, 1 hit |
PROSITEi | View protein in PROSITE PS00363, BACTERIAL_PQQ_1, 1 hit PS00364, BACTERIAL_PQQ_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MTIRSLPAAL SPLSMAVQAV LLVSSLALAP AANAKPVTWE DIANDHLNTQ
60 70 80 90 100
NVLQYGMGTN AQRWSPLAMV NDKNVFKLTP AWSYSFGDER QRGQESQAII
110 120 130 140 150
NDGVIYVTGS YSRVFALDAK TGRRLWTYNH RLPDNIRPCC DVVNRGAAIF
160 170 180 190 200
GDKIYFGTLD ARVIALNKDT GKVVWNKKFG DHSAGYTMTG APTLIKDQKS
210 220 230 240 250
GKVLLIHGSS GDEFGVVGQL YARDPETGEE VWMRPFVEGH MGRLNGKDST
260 270 280 290 300
PTGDVKAPSW PDDPTTETGK VESWSHGGGA PWQSASFDPE TNTIIVGAGN
310 320 330 340 350
PGPWNTWART SKDGNPHDFD SLYTSGQVGV DPTTGEVKWF YQHTPNDAWD
360 370 380 390 400
FSGNNELVLF DYKDKDGKQY KATAHADRNG FFYVVDRTNG KLKNAFPFVD
410 420 430 440 450
NITWASHIDL KTGRPVENEG QRPAKPLPGE TKGKPVEVSP PFLGGKNWNP
460 470 480 490 500
MAYSQDTGLF YVPANHWKEE YWTEEVNYKK GSAYLGIGFR IKRMYEDHVG
510 520 530 540 550
SLRAMDPTTG KVVWEHNERL PLWAGVLATK GNLVFTGTGD GYFKAFNAKT
560 570 580 590 600
GEELWKFQTG SGIVSPPITW EQDGEQYIGV TVGYGGAVPL WGGDMAELTK
610 620
PVAQGGSFWV FKIPAWDTKT AKR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB333783 Genomic DNA Translation: BAF91141.1 |
Genome annotation databases
KEGGi | ag:BAF91141 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB333783 Genomic DNA Translation: BAF91141.1 |
3D structure databases
SMRi | A8R3S4 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:BAF91141 |
Enzyme and pathway databases
UniPathwayi | UPA00780;UER00767 |
Family and domain databases
CDDi | cd10277, PQQ_ADH_I, 1 hit |
InterProi | View protein in InterPro IPR034119, ADHI IPR018391, PQQ_beta_propeller_repeat IPR017512, PQQ_MeOH/EtOH_DH IPR002372, PQQ_repeat IPR011047, Quinoprotein_ADH-like_supfam IPR001479, Quinoprotein_DH_CS |
Pfami | View protein in Pfam PF01011, PQQ, 1 hit PF13360, PQQ_2, 1 hit |
SMARTi | View protein in SMART SM00564, PQQ, 5 hits |
SUPFAMi | SSF50998, SSF50998, 1 hit |
TIGRFAMsi | TIGR03075, PQQ_enz_alc_DH, 1 hit |
PROSITEi | View protein in PROSITE PS00363, BACTERIAL_PQQ_1, 1 hit PS00364, BACTERIAL_PQQ_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | QEDH_PSEPU | |
Accessioni | A8R3S4Primary (citable) accession number: A8R3S4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2012 |
Last sequence update: | January 15, 2008 | |
Last modified: | February 23, 2022 | |
This is version 58 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families