UniProtKB - A8QL56 (VSP1_OPHHA)
Protein
Alpha- and beta-fibrinogenase OhS1
Gene
N/A
Organism
Ophiophagus hannah (King cobra) (Naja hannah)
Status
Functioni
Snake venom serine protease that possesses potent fibrinogenolytic (on both alpha- (FGA) and beta-chains (FGB)) and amidolytic activities. Selectively cleaves Arg-|-Xaa or Lys-|-Xaa bonds.1 Publication
Miscellaneous
Does not hydrolyze casein. Does not clot fibrinogen or act on factor X, prothrombin and plasminogen. Does not have hemorrhagic activity (PubMed:8079373).1 Publication
Activity regulationi
Completely inhibited by NPGB, PMSF, diisopropylfluorophosphate (DFP), benzamidine and soybean trypsin inhibitor. Not inhibited by EDTA.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 65 | Charge relay systemBy similarity | 1 | |
Active sitei | 110 | Charge relay systemBy similarity | 1 | |
Active sitei | 203 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: InterPro
- toxin activity Source: UniProtKB-KW
Keywordsi
Molecular function | Fibrinogenolytic toxin, Hemostasis impairing toxin, Hydrolase, Protease, Serine protease, Toxin |
Protein family/group databases
MEROPSi | S01.481 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha- and beta-fibrinogenase OhS1 (EC:3.4.21.-)Alternative name(s): Snake venom serine protease Short name: SVSP |
Organismi | Ophiophagus hannah (King cobra) (Naja hannah) |
Taxonomic identifieri | 8665 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Elapidae › Elapinae › Ophiophagus |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
PropeptideiPRO_0000417000 | 19 – 24 | 1 Publication | 6 | |
ChainiPRO_0000417001 | 25 – 260 | Alpha- and beta-fibrinogenase OhS1Add BLAST | 236 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 31 ↔ 163 | PROSITE-ProRule annotation | ||
Glycosylationi | 44 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 50 ↔ 66 | PROSITE-ProRule annotation | ||
Glycosylationi | 79 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 98 ↔ 255 | PROSITE-ProRule annotation | ||
Glycosylationi | 117 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 121 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 142 ↔ 209 | PROSITE-ProRule annotation | ||
Disulfide bondi | 174 ↔ 188 | PROSITE-ProRule annotation | ||
Disulfide bondi | 199 ↔ 224 | PROSITE-ProRule annotation | ||
Glycosylationi | 250 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinExpressioni
Tissue specificityi
Expressed by the venom gland.
Interactioni
Subunit structurei
Monomer.
1 PublicationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 248 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 224 |
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
A8QL56-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALIRVLASL LILQLSYAVT PFDRIIGGFE CNEYEHRSLV HLYNSSGFFC
60 70 80 90 100
SGTLLNHEWV LTAAHCNRDD IQIKLGVHNV SVNYEDEQIR VPKEKLCCHS
110 120 130 140 150
TNNCTQLGQD IMLIRLNSSV NYSEHIAPLS LPSNRPSMGS VCRVMGWGLL
160 170 180 190 200
TSPEVTFPKV PHCVDINILH IQVCQAAYPS MSENYLLCAG VLEGGKDSCK
210 220 230 240 250
GDSGGPLICN REIQGIVSWG GFPCAQLLEP GVYTKVFDYI DWIEGIIAGN
260
TSVTCPSDNF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF080837 mRNA Translation: ABN72544.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF080837 mRNA Translation: ABN72544.1 |
3D structure databases
SMRi | A8QL56 |
ModBasei | Search... |
Protein family/group databases
MEROPSi | S01.481 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VSP1_OPHHA | |
Accessioni | A8QL56Primary (citable) accession number: A8QL56 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2012 |
Last sequence update: | January 15, 2008 | |
Last modified: | August 12, 2020 | |
This is version 43 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries