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Protein

Thiamine thiazole synthase

Gene

CC1G_04976

Organism
Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71Substrate; via amide nitrogenUniRule annotation1
Binding sitei100Substrate; via amide nitrogenUniRule annotation1
Binding sitei165Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei201SubstrateUniRule annotation1
Binding sitei216SubstrateUniRule annotation1
Binding sitei268Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
ORF Names:CC1G_04976
OrganismiCoprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata)
Taxonomic identifieri240176 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesPsathyrellaceaeCoprinopsis
Proteomesi
  • UP000001861 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:CC1G_04976

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004158731 – 313Thiamine thiazole synthaseAdd BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1992,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-199 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Protein-protein interaction databases

STRINGi240176.XP_001835983.2

Structurei

3D structure databases

ProteinModelPortaliA8NSD1
SMRiA8NSD1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 93Substrate bindingUniRule annotation2
Regioni278 – 280Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2960 Eukaryota
COG1635 LUCA
InParanoidiA8NSD1
KOiK03146
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

A8NSD1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPALTLEKP VETNAFPRKA ASVETSENYE GNYKFAPIEE AQVSRAMIKR
60 70 80 90 100
YFNTMYDRAI SDVVIVGAGS AGLSCAYSLA TQRPDLKITI VEAGVAPGGG
110 120 130 140 150
AWLGGQLMTP MVIRKPADAF LRELGVPYED EGNFVVVKHA ALFTSTLLSK
160 170 180 190 200
VLAKPNVVMM NATAVEDLIV HEDFAGQQRV AGVVTNWTLV ALNHDTQSCM
210 220 230 240 250
DPNTITAPVI VSATGHDGPM GAFSAKRLVS TGLLKELGNM RGLDMNRAEP
260 270 280 290 300
AIVNGTREVV PGLILTGMEL SEHDGSNRMG PTFGAMIGSG HKAAHEAIRI
310
LDRHKVVNGK VVA
Length:313
Mass (Da):33,353
Last modified:August 10, 2010 - v2
Checksum:i23B881D01C9BBADD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACS02000008 Genomic DNA Translation: EAU85759.2
RefSeqiXP_001835983.2, XM_001835931.2

Genome annotation databases

EnsemblFungiiEAU85759; EAU85759; CC1G_04976
GeneIDi6012523
KEGGicci:CC1G_04976

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACS02000008 Genomic DNA Translation: EAU85759.2
RefSeqiXP_001835983.2, XM_001835931.2

3D structure databases

ProteinModelPortaliA8NSD1
SMRiA8NSD1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi240176.XP_001835983.2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAU85759; EAU85759; CC1G_04976
GeneIDi6012523
KEGGicci:CC1G_04976

Organism-specific databases

EuPathDBiFungiDB:CC1G_04976

Phylogenomic databases

eggNOGiKOG2960 Eukaryota
COG1635 LUCA
InParanoidiA8NSD1
KOiK03146
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_COPC7
AccessioniPrimary (citable) accession number: A8NSD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: August 10, 2010
Last modified: November 7, 2018
This is version 47 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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