UniProtKB - A8MW95 (BECN2_HUMAN)
Protein
Beclin-2
Gene
BECN2
Organism
Homo sapiens (Human)
Status
Functioni
Involved in 2 distinct lysosomal degradation pathways: acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. Regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1.1 Publication
GO - Molecular functioni
- protein-containing complex binding Source: Ensembl
GO - Biological processi
- autophagosome assembly Source: GO_Central
- autophagy Source: UniProtKB
- cellular response to nitrogen starvation Source: GO_Central
- endosome to lysosome transport Source: UniProtKB
- glucose homeostasis Source: Ensembl
- G protein-coupled receptor catabolic process Source: UniProtKB
- late endosome to vacuole transport Source: GO_Central
Keywordsi
Biological process | Autophagy |
Enzyme and pathway databases
PathwayCommonsi | A8MW95 |
Names & Taxonomyi
Protein namesi | Recommended name: Beclin-21 PublicationAlternative name(s): Beclin-1 autophagy-related pseudogene 1Imported Beclin-1-like protein 1Imported |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000196289.7 |
HGNCi | HGNC:38606, BECN2 |
MIMi | 615687, gene |
neXtProti | NX_A8MW95 |
Subcellular locationi
Other locations
- Cytoplasm Curated
Other locations
- extrinsic component of membrane Source: GO_Central
- phagophore assembly site Source: GO_Central
- phosphatidylinositol 3-kinase complex, class III, type I Source: GO_Central
- phosphatidylinositol 3-kinase complex, class III, type II Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 80 | I → S: Abolishes interaction with GPRASP1/GASP1 and ability to degrade G-protein coupled receptor. Does not affect function in autophagy. 1 Publication | 1 | |
Mutagenesisi | 173 | E → L: Decreases homodimerization. Decreases interaction with ATG14. 1 Publication | 1 | |
Mutagenesisi | 187 | N → L: Increases strongly homodimerization. Decreases interaction with ATG14. 1 Publication | 1 | |
Mutagenesisi | 190 | A → L: Decreases interaction with ATG14. Increases slightly homodimerization; when associated with L-215. 1 Publication | 1 | |
Mutagenesisi | 197 | A → L: Decreases interaction with ATG14. Probably strongly increases homodimerization; when associated with L-208. 1 Publication | 1 | |
Mutagenesisi | 208 | E → L: Decreases interaction with ATG14. Probably strongly increases homodimerization; when associated with L-197. 1 Publication | 1 | |
Mutagenesisi | 211 | H → L: Increases homodimerization. Decreases interaction with ATG14. 1 Publication | 1 | |
Mutagenesisi | 215 | Y → L: Decreases interaction with ATG14. Increases slightly homodimerization; when associated with L-190. 1 Publication | 1 | |
Mutagenesisi | 222 | Q → L: Decreases homodimerization. Decreases interaction with ATG14. 1 Publication | 1 | |
Mutagenesisi | 243 | R → L: Decreases homodimerization. Decreases interaction with ATG14. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 441925 |
OpenTargetsi | ENSG00000196289 |
Miscellaneous databases
Pharosi | A8MW95, Tbio |
Polymorphism and mutation databases
BioMutai | BECN2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000332244 | 1 – 431 | Beclin-2Add BLAST | 431 |
Proteomic databases
PRIDEi | A8MW95 |
ProteomicsDBi | 2232 |
PTM databases
iPTMneti | A8MW95 |
PhosphoSitePlusi | A8MW95 |
Expressioni
Tissue specificityi
Present in fetal and adult brain (at protein level).1 Publication
Organism-specific databases
HPAi | ENSG00000196289, Not detected |
Interactioni
Subunit structurei
Homodimer (via coiled-coil domain) (PubMed:28218432).
Interacts (via coiled-coil domain) with ATG14 (via coiled-coil domain); this interaction is tighter than BECN2 self-association (PubMed:23954414, PubMed:28218432).
Interacts with AMBRA1, UVRAG and PIK3C3/VPS34; these interactions are not disrupted by starvation (PubMed:23954414). Does not interact with RUBCN (PubMed:23954414).
Interacts (via N-terminus) with GPRASP1/GASP1; the interaction is direct (PubMed:23954414).
2 PublicationsBinary interactionsi
A8MW95
With | #Exp. | IntAct |
---|---|---|
GPRASP1 [Q5JY77] | 4 | EBI-8839517,EBI-2514717 |
Protein-protein interaction databases
BioGRIDi | 137882, 1 interactor |
IntActi | A8MW95, 6 interactors |
Miscellaneous databases
RNActi | A8MW95, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | A8MW95 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 173 – 243 | Required for homodimer formation1 PublicationAdd BLAST | 71 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 125 – 248 | Sequence analysisAdd BLAST | 124 |
Sequence similaritiesi
Belongs to the beclin family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
GeneTreei | ENSGT00390000008164 |
HOGENOMi | CLU_024219_4_1_1 |
InParanoidi | A8MW95 |
OMAi | VGWNEIN |
OrthoDBi | 1085752at2759 |
Family and domain databases
Gene3Di | 1.10.418.40, 1 hit |
InterProi | View protein in InterPro IPR007243, Atg6/Beclin IPR038274, Atg6/Beclin_C_sf IPR041691, Atg6/beclin_CC IPR040455, Atg6_BARA IPR032912, BECN2 |
PANTHERi | PTHR12768, PTHR12768, 1 hit PTHR12768:SF5, PTHR12768:SF5, 1 hit |
Pfami | View protein in Pfam PF04111, APG6, 1 hit PF17675, APG6_N, 1 hit |
i Sequence
Sequence statusi: Complete.
A8MW95-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSIRFLCQR CHQALKLSGS SESRSLPAAP APTSGQAEPG DTREPGVTTR
60 70 80 90 100
EVTDAEEQQD GASSRSPPGD GSVSKGHANI FTLLGELGAM HMLSSIQKAA
110 120 130 140 150
GDIFDIVSGQ AVVDHPLCEE CTDSLLEQLD IQLALTEADS QNYQRCLETG
160 170 180 190 200
ELATSEDEAA ALRAELRDLE LEEARLVQEL EDVDRNNARA AADLQAAQAE
210 220 230 240 250
AAELDQQERQ HYRDYSALKR QQLELLDQLG NVENQLQYAR VQRDRLKEIN
260 270 280 290 300
CFTATFEIWV EGPLGVINNF RLGRLPTVRV GWNEINTAWG QAALLLLTLA
310 320 330 340 350
NTIGLQFQRY RLIPCGNHSY LKSLTDDRTE LPLFCYGGQD VFLNNKYDRA
360 370 380 390 400
MVAFLDCMQQ FKEEAEKGEL GLSLPYGIQV ETGLMEDVGG RGECYSIRTH
410 420 430
LNTQELWTKA LKFMLINFKW SLIWVASRYQ K
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM031116 mRNA Translation: ADM47406.1 BX571673 Genomic DNA No translation available. |
CCDSi | CCDS81433.1 |
RefSeqi | NP_001277622.1, NM_001290693.1 |
Genome annotation databases
Ensembli | ENST00000419583; ENSP00000488361; ENSG00000196289 |
GeneIDi | 441925 |
KEGGi | hsa:441925 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM031116 mRNA Translation: ADM47406.1 BX571673 Genomic DNA No translation available. |
CCDSi | CCDS81433.1 |
RefSeqi | NP_001277622.1, NM_001290693.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5K7B | X-ray | 2.30 | A/B/C/D | 158-250 | [»] | |
5K9L | X-ray | 2.52 | A/B/C/D | 158-250 | [»] | |
SMRi | A8MW95 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 137882, 1 interactor |
IntActi | A8MW95, 6 interactors |
PTM databases
iPTMneti | A8MW95 |
PhosphoSitePlusi | A8MW95 |
Polymorphism and mutation databases
BioMutai | BECN2 |
Proteomic databases
PRIDEi | A8MW95 |
ProteomicsDBi | 2232 |
Protocols and materials databases
Antibodypediai | 77840, 308 antibodies |
Genome annotation databases
Ensembli | ENST00000419583; ENSP00000488361; ENSG00000196289 |
GeneIDi | 441925 |
KEGGi | hsa:441925 |
Organism-specific databases
CTDi | 441925 |
DisGeNETi | 441925 |
EuPathDBi | HostDB:ENSG00000196289.7 |
GeneCardsi | BECN2 |
HGNCi | HGNC:38606, BECN2 |
HPAi | ENSG00000196289, Not detected |
MIMi | 615687, gene |
neXtProti | NX_A8MW95 |
OpenTargetsi | ENSG00000196289 |
GenAtlasi | Search... |
Phylogenomic databases
GeneTreei | ENSGT00390000008164 |
HOGENOMi | CLU_024219_4_1_1 |
InParanoidi | A8MW95 |
OMAi | VGWNEIN |
OrthoDBi | 1085752at2759 |
Enzyme and pathway databases
PathwayCommonsi | A8MW95 |
Miscellaneous databases
BioGRID-ORCSi | 441925, 0 hits in 14 CRISPR screens |
GenomeRNAii | 441925 |
Pharosi | A8MW95, Tbio |
PROi | PR:A8MW95 |
RNActi | A8MW95, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 1.10.418.40, 1 hit |
InterProi | View protein in InterPro IPR007243, Atg6/Beclin IPR038274, Atg6/Beclin_C_sf IPR041691, Atg6/beclin_CC IPR040455, Atg6_BARA IPR032912, BECN2 |
PANTHERi | PTHR12768, PTHR12768, 1 hit PTHR12768:SF5, PTHR12768:SF5, 1 hit |
Pfami | View protein in Pfam PF04111, APG6, 1 hit PF17675, APG6_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BECN2_HUMAN | |
Accessioni | A8MW95Primary (citable) accession number: A8MW95 Secondary accession number(s): E9KNW0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2008 |
Last sequence update: | October 31, 2012 | |
Last modified: | December 2, 2020 | |
This is version 79 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM