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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO) (Thermotoga lettingae)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi247Magnesium or manganese 1UniRule annotation1
Metal bindingi259Magnesium or manganese 1UniRule annotation1
Metal bindingi259Magnesium or manganese 2UniRule annotation1
Metal bindingi261Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi125 – 176ATPUniRule annotationAdd BLAST52

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTLET416591:G1G9T-1918-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:Tlet_1858
OrganismiPseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO) (Thermotoga lettingae)
Taxonomic identifieri416591 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaePseudothermotoga
Proteomesi
  • UP000002016 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000573311 – 296D-alanine--D-alanine ligaseAdd BLAST296

Interactioni

Protein-protein interaction databases

STRINGi416591.Tlet_1858

Structurei

3D structure databases

ProteinModelPortaliA8F8C8
SMRiA8F8C8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 292ATP-graspUniRule annotationAdd BLAST194

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011592
KOiK01921
OMAiYETKYTE
OrthoDBiPOG091H00GT

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

A8F8C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIAVLLGGI SRERPISLKS GENVLKALKK LGHDVVPIDV DENFLEIAPK
60 70 80 90 100
LKEFEVVFNA LHGHFGEDGT VQAILDWVGV SYTGSKVLAS AICFDKVMTY
110 120 130 140 150
RVLDGYVNFP EYTIVKKPVK ESPYGFPCVI KPRKEGSSIG VHICDNSNQL
160 170 180 190 200
YNDLSEELKK YNEMMIQRYI EGRELTVSIL EIGKPQILPV LELKPKRRFY
210 220 230 240 250
DYTAKYVSGM TEFILPAPLS VEEYQKVANS SLRAFELCGC EGFARVDGIL
260 270 280 290
KDNVFYVLEL NTIPGLTDLS DMPASAKAAG MSFEELVDAI IQTAVR
Length:296
Mass (Da):33,012
Last modified:November 13, 2007 - v1
Checksum:i8C8348F0CDFE5EB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000812 Genomic DNA Translation: ABV34412.1
RefSeqiWP_012003888.1, NC_009828.1

Genome annotation databases

EnsemblBacteriaiABV34412; ABV34412; Tlet_1858
KEGGitle:Tlet_1858

Similar proteinsi

Entry informationi

Entry nameiDDL_PSELT
AccessioniPrimary (citable) accession number: A8F8C8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: February 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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