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UniProtKB - A8CG78 (PA2A2_DABSI)
Protein
Acidic phospholipase A2 DsM-a2/DsM-a2'
Gene
N/A
Organism
Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Status
Functioni
Exhibits high hydrolytic activities and shows strong preference for the anionic micelles (dPPC with deoxycholate) to the zwitterionic micelles (dPPC with Triton X-100). PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
1 PublicationCaution
Sequence obtained after translation (DsM-a2) and sequence directly sequenced from protein (Dsm-a2', AA 17-29) correspond exactly. However, the mass found for the protein (13810) does not correspond to the expected mass calculated after translation (13810) (PubMed:17611171).1 Publication
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+PROSITE-ProRule annotationEC:3.1.1.4PROSITE-ProRule annotation
Cofactori
Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 43 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 45 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 47 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 63 | By similarity | 1 | |
Metal bindingi | 64 | CalciumBy similarity | 1 | |
Active sitei | 105 | By similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phospholipase A2 activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- arachidonic acid secretion Source: InterPro
- lipid catabolic process Source: UniProtKB-KW
- phospholipid metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Toxin |
Biological process | Lipid degradation, Lipid metabolism |
Ligand | Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Acidic phospholipase A2 DsM-a2/DsM-a2' (EC:3.1.1.4)Short name: svPLA2 Alternative name(s): Phosphatidylcholine 2-acylhydrolase |
Organismi | Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis) |
Taxonomic identifieri | 343250 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Viperinae › Daboia |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | 1 PublicationAdd BLAST | 16 | |
ChainiPRO_0000419219 | 17 – 138 | Acidic phospholipase A2 DsM-a2/DsM-a2'Add BLAST | 122 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 42 ↔ 131 | By similarity | ||
Disulfide bondi | 44 ↔ 60 | By similarity | ||
Disulfide bondi | 59 ↔ 111 | By similarity | ||
Disulfide bondi | 65 ↔ 138 | By similarity | ||
Disulfide bondi | 66 ↔ 104 | By similarity | ||
Disulfide bondi | 73 ↔ 97 | By similarity | ||
Disulfide bondi | 91 ↔ 102 | By similarity |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
A8CG78-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTLWIVAVC LIGVEGNLYQ FGEMINQKTG NFGLLSYVYY GCYCGWGGKG
60 70 80 90 100
KPQDATDRCC FVHDCCYGRV KGCDPKTATY SYSFENGDIV CGGDDPCLRA
110 120 130
VCECDRVAAI CFRENMNTYD KKYMLYSIFD CKEESDQC
Mass spectrometryi
Molecular mass is 13810 Da. Determined by ESI. in Dsm-a2'.1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ090654 mRNA Translation: AAZ53176.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ090654 mRNA Translation: AAZ53176.1 |
3D structure databases
SMRi | A8CG78 |
ModBasei | Search... |
Family and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PA2A2_DABSI | |
Accessioni | A8CG78Primary (citable) accession number: A8CG78 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2012 |
Last sequence update: | November 13, 2007 | |
Last modified: | February 23, 2022 | |
This is version 49 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families