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UniProtKB - A7ZIA4 (FRMA_ECO24)
Protein
S-(hydroxymethyl)glutathione dehydrogenase
Gene
frmA
Organism
Escherichia coli O139:H28 (strain E24377A / ETEC)
Status
Functioni
Has high formaldehyde dehydrogenase activity in the presence of glutathione and catalyzes the oxidation of normal alcohols in a reaction that is not GSH-dependent.
By similarityCatalytic activityi
Cofactori
Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 40 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 62 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 92 | Zinc 2By similarity | 1 | |
Metal bindingi | 95 | Zinc 2By similarity | 1 | |
Metal bindingi | 98 | Zinc 2By similarity | 1 | |
Metal bindingi | 106 | Zinc 2By similarity | 1 | |
Metal bindingi | 169 | Zinc 1; catalyticBy similarity | 1 |
GO - Molecular functioni
- alcohol dehydrogenase (NAD+) activity Source: UniProtKB-EC
- S-(hydroxymethyl)glutathione dehydrogenase NAD activity Source: UniProtKB-EC
- S-(hydroxymethyl)glutathione dehydrogenase NADP activity Source: UniProtKB-EC
- zinc ion binding Source: InterPro
GO - Biological processi
- ethanol oxidation Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, NAD, Zinc |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:frmA Ordered Locus Names:EcE24377A_0381 |
Organismi | Escherichia coli O139:H28 (strain E24377A / ETEC) |
Taxonomic identifieri | 331111 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000341286 | 1 – 369 | S-(hydroxymethyl)glutathione dehydrogenaseAdd BLAST | 369 |
Interactioni
Subunit structurei
Homodimer.
By similarityFamily & Domainsi
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_026673_14_0_6 |
OMAi | RVKMIAT |
Family and domain databases
CDDi | cd08300, alcohol_DH_class_III, 1 hit |
InterProi | View protein in InterPro IPR013149, ADH-like_C IPR014183, ADH_3 IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02818, adh_III_F_hyde, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
i Sequence
Sequence statusi: Complete.
A7ZIA4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSRAAVAFA PGKPLEIVEI DVAPPKKGEV LIKVTHTGVC HTDAFTLSGD
60 70 80 90 100
DPEGVFPVVL GHEGAGVVVE VGEGVTSVKP GDHVIPLYTA ECGECEFCRS
110 120 130 140 150
GKTNLCVAVR ETQGKGLMPD GTTRFSYNGQ PLYHYMGCST FSEYTVVAEV
160 170 180 190 200
SLAKINPEAN HEHVCLLGCG VTTGIGAVHN TAKVQPGDSV AVFGLGAIGL
210 220 230 240 250
AVVQGARQAK AGRIIAIDTN PKKFDLARRF GATDCINPND YDKPIKDVLL
260 270 280 290 300
DINKWGIDHT FECIGNVNVM RAALESAHRG WGQSVIIGVA GSGQEISTRP
310 320 330 340 350
FQLVTGRVWK GSAFGGVKGR SQLPGMVEDA MKGDIDLEPF VTHTMSLDEI
360
NDAFDLMHEG KSIRTVIRY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000800 Genomic DNA Translation: ABV17265.1 |
RefSeqi | WP_000842102.1, NC_009801.1 |
Genome annotation databases
EnsemblBacteriai | ABV17265; ABV17265; EcE24377A_0381 |
GeneIDi | 66671339 |
KEGGi | ecw:EcE24377A_0381 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000800 Genomic DNA Translation: ABV17265.1 |
RefSeqi | WP_000842102.1, NC_009801.1 |
3D structure databases
AlphaFoldDBi | A7ZIA4 |
SMRi | A7ZIA4 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | ABV17265; ABV17265; EcE24377A_0381 |
GeneIDi | 66671339 |
KEGGi | ecw:EcE24377A_0381 |
Phylogenomic databases
HOGENOMi | CLU_026673_14_0_6 |
OMAi | RVKMIAT |
Family and domain databases
CDDi | cd08300, alcohol_DH_class_III, 1 hit |
InterProi | View protein in InterPro IPR013149, ADH-like_C IPR014183, ADH_3 IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR02818, adh_III_F_hyde, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FRMA_ECO24 | |
Accessioni | A7ZIA4Primary (citable) accession number: A7ZIA4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | October 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 86 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |