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Protein

Biotin synthase

Gene

bioB

Organism
Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi65Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi69Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi72Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi109Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi141Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi201Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi271Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:RBAM_018250
OrganismiBacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)
Taxonomic identifieri326423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus amyloliquefaciens group
Proteomesi
  • UP000001120 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003812141 – 339Biotin synthaseAdd BLAST339

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA7Z5B2
SMRiA7Z5B2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
KOiK01012
OMAiADRFCMG

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

A7Z5B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQWMDLAER VLDGAEVTNQ EALAILNCPD EDLLLLMHAA FHIRKRFYGK
60 70 80 90 100
KVKLNMIMNA KSGLCPENCG YCSQSSISKA PIESYRMVDK HILLKGAKRA
110 120 130 140 150
HDLNIGTYCI VASGRGPSNR EVDQVVDAVK EIKETYGLKI CACLGLLKPE
160 170 180 190 200
QAERLKQAGV DRYNHNINTS QRNHSNITTS HTYDDRVNTV ETAKQSGMSP
210 220 230 240 250
CSGVIVGMKE TKQDVIDMAN SLKALDADSI PVNFLHAIDG TPLEGVSELH
260 270 280 290 300
PVYCLKVLAL FRFINPSKEI RISGGREVNL RSLQPLGLYA ANSIFVGDYL
310 320 330
TTAGQQETED HQMLHDLGFE VESVEEMKAG LQSACNTEV
Length:339
Mass (Da):37,483
Last modified:October 23, 2007 - v1
Checksum:i8DC65BA9FA6A17CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000560 Genomic DNA Translation: ABS74188.1
RefSeqiWP_007410132.1, NC_009725.1

Genome annotation databases

EnsemblBacteriaiABS74188; ABS74188; RBAM_018250
KEGGibay:RBAM_018250

Similar proteinsi

Entry informationi

Entry nameiBIOB_BACVZ
AccessioniPrimary (citable) accession number: A7Z5B2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 23, 2007
Last modified: July 18, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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