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UniProtKB - A7Y7W2 (PO5F3_CHICK)
Protein
POU domain, class 5, transcription factor 3
Gene
POU5F3
Organism
Gallus gallus (Chicken)
Status
Functioni
Required for the maintenance of pluripotency and self-renewal of embryonic stem cells. Transcriptional activator that binds the DNA consensus sequence 5'-ATGCAAAT-3'.
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 264 – 323 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- positive regulation of stem cell population maintenance Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: POU domain, class 5, transcription factor 3 |
Gene namesi | Name:POU5F3 Synonyms:POU2, POUV |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleus Source: AgBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000433626 | 1 – 389 | POU domain, class 5, transcription factor 3Add BLAST | 389 |
Expressioni
Developmental stagei
Widely expressed during embryonic development (at protein level). In pre-primitive streak stage embryos, expressed in the epiblast and in a salt-and-pepper fashion in the forming hypoblast (stages XI and XIII) (at protein level). As the primitive streak starts to form, strongly expressed in the epiblast of the streak itself (stage XIV) and in the mesoderm emerging from it, whereas expression in the lower layer tends to decrease (stages 2 through 4+). Expression in the area opaca is lost by stage 3+. At later stages, continues to be expressed in the mesoderm, but not detected in the endoderm (stages 5 through 8). At stage 5, expressed in all cells of the germinal crescent. At stage 8 and subsequently, strongly expressed in the neural plate and neural tube with particularly strong expression in the anterior hindbrain/posterior midbrain. At stage 9 and subsequently, still expressed in neural tissue and in primordial germ cells (at protein level). At stage 33, still expressed in germ cells. At stages 42-43, expressed in male and female gonads, as well as in spleen and brain, but at much lower levels than in proliferating embryonic stem cells.2 Publications
Inductioni
Strongly down-regulated during embryonic stem cell differentiation induced either by retinoic acid treatment, or by cell adhesion prevention leading to embryoid body formation.1 Publication
Gene expression databases
Bgeei | ENSGALG00000026574, Expressed in testis and 1 other tissue |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 170 – 244 | POU-specificPROSITE-ProRule annotationAdd BLAST | 75 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 88 | DisorderedSequence analysisAdd BLAST | 88 | |
Regioni | 145 – 177 | DisorderedSequence analysisAdd BLAST | 33 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 145 – 162 | Polar residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Belongs to the POU transcription factor family.UniRule annotation
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG3802, Eukaryota |
OrthoDBi | 668779at2759 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
i Sequence
Sequence statusi: Complete.
A7Y7W2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFSPDGGLPA APFGLLPDGG PPFPRGGYDG AAAQQLFFPF ASEPDGARDA
60 70 80 90 100
ATARAWLPPP AGPPAKAEAR PARPCRQGSP EPRAAPPATP CCGPAWAAPP
110 120 130 140 150
WPGPAPPAAT AVPGPPFPGP AAAAFPAAPG HALCPAALQP SSGGLANLGS
160 170 180 190 200
SGSSSGAASE GGHSSDSGDE DAPTSEELEQ FAKDLKHKRI MLGFTQADVG
210 220 230 240 250
LALGTLYGKM FSQTTICRFE ALQLSFKNMC KLKPLLQRWL NEAENTDNMQ
260 270 280 290 300
EMCNAEQVLA QARKRKRRTS IETNVKGTLE SFFRKCVKPS PQEISQIAED
310 320 330 340 350
LNLDKDVVRV WFCNRRQKGK RLLLPFGNES EGVMYDMNQS LVPPGLPIPV
360 370 380
TSQGYSLAPS PPVYMPPFHK AEMFPPPLQP GISMNNSSH
Sequence cautioni
The sequence ABK27428 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AADN03007288 Genomic DNA No translation available. DQ867024 Genomic DNA Translation: ABK27428.1 Sequence problems. LC016616 Genomic DNA Translation: BAR64207.1 LC016620 mRNA Translation: BAR64208.1 BR001258 mRNA Translation: FAA01189.1 |
RefSeqi | NP_001103648.1, NM_001110178.1 NP_001296301.1, NM_001309372.1 |
Genome annotation databases
GeneIDi | 427781 |
KEGGi | gga:427781 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AADN03007288 Genomic DNA No translation available. DQ867024 Genomic DNA Translation: ABK27428.1 Sequence problems. LC016616 Genomic DNA Translation: BAR64207.1 LC016620 mRNA Translation: BAR64208.1 BR001258 mRNA Translation: FAA01189.1 |
RefSeqi | NP_001103648.1, NM_001110178.1 NP_001296301.1, NM_001309372.1 |
3D structure databases
AlphaFoldDBi | A7Y7W2 |
SMRi | A7Y7W2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000041055 |
Genome annotation databases
GeneIDi | 427781 |
KEGGi | gga:427781 |
Organism-specific databases
CTDi | 30333 |
Phylogenomic databases
eggNOGi | KOG3802, Eukaryota |
OrthoDBi | 668779at2759 |
Miscellaneous databases
PROi | PR:A7Y7W2 |
Gene expression databases
Bgeei | ENSGALG00000026574, Expressed in testis and 1 other tissue |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PO5F3_CHICK | |
Accessioni | A7Y7W2Primary (citable) accession number: A7Y7W2 Secondary accession number(s): A0A0E4BYL6 R4GG91 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 16, 2015 |
Last sequence update: | September 16, 2015 | |
Last modified: | May 25, 2022 | |
This is version 89 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families